FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12142173
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC691090.5691650086026612TruSeq Adapter, Index 7 (100% over 50bp)
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT227860.1876599847490231No Hit
CTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT155110.12774484435364247No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCC152440.12554589693294604No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA147460.12144448938423132No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTAT146940.12101622996147396No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT130320.10732839994949833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)