FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12142173
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGATGAGTGTAGATCTC725080.5971583504863586Illumina Single End PCR Primer 1 (96% over 32bp)
CGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAA308640.25418843892275295No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG137280.113060487607943No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG128330.10568948408163842No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT122240.10067390738049936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)