FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457220
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103993712.296440201390055No Hit
CAACGGAATCCCAAAAGCAGCTG5570176.5862895845206815No Hit
AACATTCAACGCTGTCGGTGAGT3118343.6871927181745305No Hit
AAGCTGCCAGTTGAAGAACTGT3085983.648929553683125No Hit
TTCAAGTAATCCAGGATAGGCT2749393.250938251576759No Hit
CCCCCACTGCTAAATTTGACTG2731733.2300566852937487No Hit
TAGCAGCACGTAAATATTGGCG2684213.1738680086364073No Hit
AACATTCAACGCTGTCGGTGAG1632291.9300550299034436No Hit
TAGCTTATCAGACTGATGTTGAC1296961.533553579072083No Hit
TAGCTTATCAGACTGATGTTGA1160011.3716209345387727No Hit
TAGCTTATCAGACTGATGTTGACT1101281.302177311220472No Hit
TATTGCACTTGTCCCGGCCTGTAT900491.064758868753562No Hit
TATTGCACTTGTCCCGGCCTGTAA816660.9656364620998389No Hit
GCCTGTCTGAGCGTCGCT808060.9554676359371047No Hit
TATTGCACTTGTCCCGGCCTGT750370.8872537311315065No Hit
TGAGGTAGTAGGTTGTATAGTT696520.8235803254497341No Hit
TGTAAACATCCTTGACTGGAAGCT692350.8186496271824547No Hit
AGCAGCATTGTACAGGGCTATGA571180.6753755962361154No Hit
CATAAAGTAGAAAGCACTACT509470.6024083564102625No Hit
CTCCCACTGCTTCACTTGACTAG490090.5794930248947053No Hit
TTCTCACTACTGCACTTGACTAG488720.5778731072385489No Hit
TATTGCACTTGTCCCGGCCTGTA473000.559285438950388No Hit
TTCAAGTAATTCAGGATAGGTT436510.5161388730575769No Hit
CTTCTCACTACTGCACTTGACTAG435430.5148618576790008No Hit
TGAGGTAGTAGATTGTATAGTT425780.5034514887870956No Hit
ACTGGACTTGGAGTCAGAAGGC396830.4692203821113794No Hit
TGTAAACATCCCCGACTGGAAGCT369390.43677473212237594No Hit
CCCATAAAGTAGAAAGCACTAC361090.4269606324536905No Hit
CGCCTGTCTGAGCGTCGCT355860.4207765672407718No Hit
CAACGGAATCCCAAAAGCAGCTGT348490.4120621197036378No Hit
TGTAAACATCCTACACTCTCAGCT302460.3576352513000726No Hit
CCCCCCACTGCTAAATTTGACTGG272060.3216896332364536No Hit
TCCTGTCTGAGCGTCGCT260720.30828097176140623No Hit
CATTGCACTTGTCTCGGTCTGA255230.3017894769203119No Hit
CTCCCACTGCTTCACTTGACTAGCCT254520.3009499575510629No Hit
CCCCCCACTGCTAAATTTGACTGGCT240540.2844197029283855No Hit
TGTCAGTTTGTCAAATACCCCAA233520.27611910296764186No Hit
AACATTCAACGCTGTCGGTGAGTT231290.2734823026952119No Hit
AACCGGGCGGAAACACCA227900.26947389331245963No Hit
CCCATAAAGTAGAAAGCACT224640.26561919874379525No Hit
CCCCCACTGCTAAATTTGACTGGCTT224570.26553642922851717No Hit
TCGTACGACTCTTAGCGG219470.25950607882968635No Hit
ACCCCCCACTGCTAAATTTGACTG191870.2268712413771901No Hit
TGAGGTAGTAGTTTGTACAGTT180000.2128358964293231No Hit
CAACACCAGTCGATGGGCTGT177980.21044740470272738No Hit
CACCCGGCTGTGTGCACATGTGC174730.20660453435053125No Hit
CTACGCCTGTCTGAGCGTCGCT174470.20629710472235557No Hit
GACCCGCGGGCGCTCTCCAGTCCT173390.20502008934377963No Hit
TTCACAGTGGCTAAGTTCCG167920.19855224293562185No Hit
TGAGGTAGTAGTTTGTGCTGTT157400.18611316721097476No Hit
TTCAAGTAATCCAGGATAGGCTT152380.1801774105438903No Hit
TGTCAGTTTGTCAAATACCCCA151130.17869938348535336No Hit
TACGCCTGTCTGAGCGTCGCT150960.17849837180539233No Hit
TGTAAACATCCTACACTCAGCT148150.1751757669778012No Hit
TAGCTTATCAGACTGATGTTGACA147460.1743598960414888No Hit
TAGCAGCACGTAAATATTGGCGT145410.1719359316654882No Hit
CAAAGAATTCTCCTTTTGGGCT144280.17059979520457078No Hit
TAGCAGCACGTAAATATTGGC142550.16855420575555563No Hit
AACGGAATCCCAAAAGCAGCTG140560.16620118667836475No Hit
TTCAAGTAATCCAGGATAGGC137300.16234649210970037No Hit
TATTGCACTTGTCCCGGCCTGTT135180.15983975821842164No Hit
TGTAAACATCCCCGACTGGAAGC133740.15813707104698707No Hit
CAACGGAATCCCAAAAGCAGCT133140.15742761805888933No Hit
GGAATACCGGGTGCTGTAGGCTTT125660.14858310414060413No Hit
AACATTCAACGCTGTCGGTGA122420.1447520580048763No Hit
ACCGGGTGCTGTAGGCTT121070.14315578878165638No Hit
AGCAGCATTGTACAGGGCTATGT119650.14147675004315838No Hit
CAAAGAATTCTCCTTTTGGGCTT117370.13878082868838695No Hit
TCCCACTGCTTCACTTGACTAG116430.13766935234036717No Hit
AGCAGCATTGTACAGGGCTATG112640.13318797429888307No Hit
AGCTCGGTCTGAGGCCCCTCAGT111910.13232480649669748No Hit
CACTAGATTGTGAGCTCCTGGA110860.13108326376752644No Hit
CAAAGTGCTGTTCGTGCAGGT109670.12967618200779926No Hit
ACGCCTGTCTGAGCGTCGCT107720.1273704597964816No Hit
GGGCTACGCCTGTCTGAGCGTCGCT107580.12720492076592546No Hit
GCGTTCGCGCTTTCCCCTG104760.12387049172186607No Hit
TTCACAGTGGCTAAGTTCCGC104480.12353941366075377No Hit
TATTGCACTTGTCCCGGCCTGTAG103290.12213233190102657No Hit
ACTGGACTTGGAGTCAGAAGGCA103290.12213233190102657No Hit
TGATGTAGTAGGTTGTATAGTT99620.11779284445716204No Hit
ACCGGGTGCTGTAGGCTTT97820.11566448549286883No Hit
ACCACAGGGTAGAACCACGGAC97290.11503780202004914No Hit
CCCCCCACTGCTAAATTTGACTGTA97190.1149195598553662No Hit
ACTGGACTTGGAGTCAGAAGG96710.11435199746488797No Hit
GGAATACCGGGTGCTGTAGGCTT96570.11418645843433185No Hit
CCCCCCACTGCTAAATTTGACTGT95170.11253106812877045No Hit
TTCACAGTGGCTAAGTTCTGC94990.11231823223234111No Hit
ACTGGACTTGGAGTCAGAAGGCT94330.11153783394543361No Hit
TTATAAAGCAATGAGACTGATT92860.10979967412459413No Hit
AACATTCAACGCTGTCGGTGAGA92030.1088182641577256No Hit
CTCCCACTGCTTCACTTGACTA91850.10860542826129627No Hit
TGAGGTAGTAGGTTGTGTGGTT90150.106595311461686No Hit
ACCGGGTGCTGTAGGCTTA89150.10541288981485641No Hit
CACTAGATTGTGAGCTCCTGGAA87600.10358013626227058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)