FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8682308
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG126793114.603616918450715No Hit
CAACGGAATCCCAAAAGCAGCTG6635177.642173025882058No Hit
CCCCCACTGCTAAATTTGACTG2965513.415577977652947No Hit
AACATTCAACGCTGTCGGTGAGT2748183.165264351368323No Hit
GGAATACCGGGTGCTGTAGGCTTT1889602.176379828957922No Hit
TTCAAGTAATCCAGGATAGGCT1830632.1084601007013344No Hit
AACATTCAACGCTGTCGGTGAG1805672.0797119844170466No Hit
TAGCAGCACGTAAATATTGGCG1705581.964431577410062No Hit
AAGCTGCCAGTTGAAGAACTGT1353471.5588827302602026No Hit
TATTGCACTTGTCCCGGCCTGTAT1335141.5377708323639292No Hit
TATTGCACTTGTCCCGGCCTGTAA1230811.4176069312445492No Hit
GGAATACCGGGTGCTGTAGGCTT1228901.4154070553590128No Hit
TAGCTTATCAGACTGATGTTGACT937161.0793904109368153No Hit
TAGCTTATCAGACTGATGTTGAC825960.9513138672343805No Hit
TAGCTTATCAGACTGATGTTGA712550.8206919174026076No Hit
TATTGCACTTGTCCCGGCCTGT675290.7777770611224574No Hit
TATTGCACTTGTCCCGGCCTGTA561610.6468441340712631No Hit
TGAATACCGGGTGCTGTAGGCTTT463370.5336944969010544No Hit
AGCAGCATTGTACAGGGCTATGA455180.5242615212452726No Hit
CTCCCACTGCTTCACTTGACTAG444520.5119836799155248No Hit
TCGTACGACTCTTAGCGG435970.5021360679671811No Hit
TGTAAACATCCTTGACTGGAAGCT417620.48100113472132067No Hit
ACTGGACTTGGAGTCAGAAGGC413220.4759333578122315No Hit
CAACGGAATCCCAAAAGCAGCTGT402770.46389738765314475No Hit
TGAGGTAGTAGGTTGTATAGTT397630.4579773028093452No Hit
CCCCCACTGCTAAATTTGACTGGCTT373110.42973596421596655No Hit
TTAATGCTAATCGTGATAGGGGTT340300.3919464732188722No Hit
GGAATACCGGGTGCTGTAGGCTTA339610.3911517536581287No Hit
TGTAAACATCCCCGACTGGAAGCT336920.3880534991387083No Hit
GACCCGCGGGCGCTCTCCAGTCCT326290.3758102108333406No Hit
CCCCCCACTGCTAAATTTGACTGGCT315010.362818273666403No Hit
CCCATAAAGTAGAAAGCACTAC312950.3604456326589658No Hit
CTTCTCACTACTGCACTTGACTAG311990.3593399358788009No Hit
CACCCGGCTGTGTGCACATGTGC305900.35232567192962977No Hit
TGAATACCGGGTGCTGTAGGCTT299040.34442454702136804No Hit
TGAGGTAGTAGATTGTATAGTT298480.3437795572329385No Hit
TTCTCACTACTGCACTTGACTAG289810.3337937331870742No Hit
CATAAAGTAGAAAGCACTACT289010.33287231920360344No Hit
TTCAAGTAATTCAGGATAGGTT285450.3287720269771586No Hit
CTCCCACTGCTTCACTTGACTAGCCT278490.3207557253209631No Hit
CAACGGAATCCCAAAAGCAGCT273290.31476653442840313No Hit
CCCCCCACTGCTAAATTTGACTGG263310.3032718949846055No Hit
ACCCCCCACTGCTAAATTTGACTG255440.29420748492221194No Hit
AGCTCGGTCTGAGGCCCCTCAGT212570.24483121308297287No Hit
CATTGCACTTGTCTCGGTCTGA189880.2186976089767836No Hit
CAAAGAATTCTCCTTTTGGGCT187560.2160255084247184No Hit
GGAATACCGGGTGCTGTAGGCT187430.2158757786524044No Hit
AACATTCAACGCTGTCGGTGAGTT185350.21348010229538047No Hit
CACTAGATTGTGAGCTCCTGGAA179270.2064773560210027No Hit
ATCCCGGACGAGCCCCCA176980.20383980849331768No Hit
CGCCTGTCTGAGCGTCGCT171270.19726321618629516No Hit
CCCATAAAGTAGAAAGCACT171220.19720562781232825No Hit
TATTGCACTTGTCCCGGCCTGTAG170750.19666429709703914No Hit
CACTAGATTGTGAGCTCCTGGA170710.19661822639786566No Hit
TCGTTTCCCGGGCGGCGCACCA167850.1933241714069577No Hit
TCGTTTCCCGGCCAATGCACCA166490.1917577676350574No Hit
TGTAAACATCCTACACTCTCAGCT163600.1884291596197693No Hit
AACCGGGCGGAAACACCA160100.18439797344208475No Hit
CCCCCCACTGCTAAATTTGACTGT156660.1804358933131605No Hit
CTACGCCTGTCTGAGCGTCGCT154860.1783627118503513No Hit
TATTGCACTTGTCCCGGCCTGTT149410.1720855790879568No Hit
ACTGGACTTGGAGTCAGAAGGCA145560.1676512742925038No Hit
AACGGAATCCCAAAAGCAGCTG143940.16578541097597552No Hit
CCCCCCACTGCTAAATTTGACTGTA143510.16529015095986No Hit
ACCGGGTGCTGTAGGCTTA141200.1626295680825882No Hit
CCCCCCACTGCTAAATTTGACTGA135470.1560299404259789No Hit
TAGCTTATCAGACTGATGTTGACA130820.15067422164705513No Hit
TATTGCACTTGTCCCGGCCTGTTT130800.15065118629746838No Hit
ACTGGACTTGGAGTCAGAAGGCT126340.1455143033396189No Hit
CAAAGAATTCTCCTTTTGGGCTT122860.14150615251152113No Hit
AACACCCTGATTGCTCCTGTCTGATT121870.14036590270697608No Hit
CAACACCAGTCGATGGGCTGT111380.12828386184871582No Hit
TATTGCACTTGTCCCGGCCTGTAAT111270.12815716742598857No Hit
TTCAAGTAATCCAGGATAGGCTT110540.1273163771660715No Hit
TAGCAGCACGTAAATATTGGC108920.12545051384954323No Hit
TGTAAACATCCCCGACTGGAAGC105930.12200672908632129No Hit
GCCTGTCTGAGCGTCGCT105640.12167271651731314No Hit
ACCGGGTGCTGTAGGCTTT105290.12126959789954467No Hit
ACCGGGTGCTGTAGGCTT103990.11977230017640472No Hit
TGAGGTAGTAGTTTGTACAGTT102130.1176300126648352No Hit
AGCAGCATTGTACAGGGCTATGT98830.11382917998301835No Hit
GACCCGCGGGCGCTCTCCAGTCCTT97860.11271196552806007No Hit
CCCCCACTGCTAAATTTGACTGGCT93550.10774784769211138No Hit
TACGCCTGTCTGAGCGTCGCT91570.10546734808302123No Hit
TGAGGTAGTAGTTTGTGCTGTT91030.10484539364417847No Hit
TGTCAGTTTGTCAAATACCCCAA90600.10435013362806295No Hit
TGAGAACTGAATTCCATGGGTT87860.10119429073467562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)