FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7819996
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG80015510.232166359164378No Hit
CAACGGAATCCCAAAAGCAGCTG5038876.443571070880343No Hit
AACATTCAACGCTGTCGGTGAGT3287314.2037233778636205No Hit
CCCCCACTGCTAAATTTGACTG2569823.2862165146887543No Hit
TTCAAGTAATCCAGGATAGGCT2201242.814886350325499No Hit
TAGCAGCACGTAAATATTGGCG1937372.477456510207934No Hit
AACATTCAACGCTGTCGGTGAG1891952.4193746390663113No Hit
AAGCTGCCAGTTGAAGAACTGT1863702.383249300894783No Hit
TAGCTTATCAGACTGATGTTGA906961.159795989665468No Hit
TAGCTTATCAGACTGATGTTGAC878561.1234788355390464No Hit
TAGCTTATCAGACTGATGTTGACT802561.02629208505989No Hit
CGCCTGTCTGAGCGTCGCT748320.9569314357705553No Hit
TGAGGTAGTAGGTTGTATAGTT663890.8489646286264085No Hit
TATTGCACTTGTCCCGGCCTGT660710.8448981303826754No Hit
TATTGCACTTGTCCCGGCCTGTAT622630.7962024533004877No Hit
TATTGCACTTGTCCCGGCCTGTAA619010.7915732949224016No Hit
AGCAGCATTGTACAGGGCTATGA484750.6198852275627762No Hit
TGTAAACATCCTTGACTGGAAGCT445860.5701537443241659No Hit
CATAAAGTAGAAAGCACTACT438620.5608954275679936No Hit
TATTGCACTTGTCCCGGCCTGTA432890.5535680580910783No Hit
TCGTACGACTCTTAGCGG426900.5459082076256816No Hit
ACTGGACTTGGAGTCAGAAGGC415230.5309849263350007No Hit
TTAATGCTAATCGTGATAGGGGTT347580.44447593067822544No Hit
TGAGGTAGTAGATTGTATAGTT328680.42030712036169837No Hit
GCCTGTCTGAGCGTCGCT325520.41626619757861766No Hit
TGTAAACATCCCCGACTGGAAGCT305630.39083140195979643No Hit
ACCGGGTGCTGTAGGCTT304590.38950147800587115No Hit
TTCAAGTAATTCAGGATAGGTT293990.37594648386009405No Hit
CTACGCCTGTCTGAGCGTCGCT291130.3722891929867995No Hit
CAACGGAATCCCAAAAGCAGCTGT291000.37212295249255883No Hit
TACGCCTGTCTGAGCGTCGCT285210.36471885663368625No Hit
AACCGGGCGGAAACACCA278740.3564451951126318No Hit
CTCCCACTGCTTCACTTGACTAG267450.3420078475743466No Hit
CCCATAAAGTAGAAAGCACTAC261950.33497459589493395No Hit
TTCTCACTACTGCACTTGACTAG255910.32725080677790624No Hit
AACATTCAACGCTGTCGGTGAGTT249250.3187341783806539No Hit
ACCGGGTGCTGTAGGCTTT246410.31510246296801175No Hit
CCCATAAAGTAGAAAGCACT239770.3066114100314118No Hit
CCCCCACTGCTAAATTTGACTGGCTT226480.28961651642788566No Hit
GGAATACCGGGTGCTGTAGGCTTT221080.2827111420517351No Hit
CATTGCACTTGTCTCGGTCTGA211560.2705372227811881No Hit
CTTCTCACTACTGCACTTGACTAG202310.2587085722294487No Hit
CCCCCCACTGCTAAATTTGACTGGCT199860.25557557829952854No Hit
ACGCCTGTCTGAGCGTCGCT195320.24976994873143155No Hit
CCCCCCACTGCTAAATTTGACTGG194920.2492584395183834No Hit
CAACACCAGTCGATGGGCTGT192290.24589526644259152No Hit
GACCCGCGGGCGCTCTCCAGTCCT186850.23893874114513614No Hit
ACCGGGTGCTGTAGGCTTA182690.23361904532943498No Hit
TGTAAACATCCTACACTCTCAGCT172950.22116379599171151No Hit
GGAATACCGGGTGCTGTAGGCTT164940.21092082400042148No Hit
CTCCCACTGCTTCACTTGACTAGCCT159060.20340163856861307No Hit
AACGGAATCCCAAAAGCAGCTG153830.196713655608008No Hit
TGAGGTAGTAGTTTGTACAGTT148780.19025585179327456No Hit
CAACGGAATCCCAAAAGCAGCT145080.1855243915725788No Hit
CACCCGGCTGTGTGCACATGTGC144880.1852686369660547No Hit
AGCTCGGTCTGAGGCCCCTCAGT140790.18003845526263698No Hit
TGAGGTAGTAGTTTGTGCTGTT129730.16589522552185448No Hit
TCCTGTCTGAGCGTCGCT128620.16447578745564576No Hit
TTCACAGTGGCTAAGTTCCG126750.16208448188464547No Hit
ACCCCCCACTGCTAAATTTGACTG126690.16200775550268826No Hit
TGAGGTAGTAGGTTGTGTGGTT125010.15985941680788585No Hit
TATTGCACTTGTCCCGGCCTGTT120710.1543606927676178No Hit
GCGTTCGCGCTTTCCCCTG115140.14723792697592172No Hit
TTCAAGTAATCCAGGATAGGCTT112060.14329930603545066No Hit
ACTGGACTTGGAGTCAGAAGGCA111990.14320979192316724No Hit
ATCCCGGACGAGCCCCCA110250.1409847268464076No Hit
TAGCTTATCAGACTGATGTTGACA109460.13997449615063742No Hit
ACTGGACTTGGAGTCAGAAGGCT105850.13535812550287749No Hit
AGCAGCATTGTACAGGGCTATG104610.13377244694242812No Hit
TGTCAGTTTGTCAAATACCCCAA104510.13364456963916607No Hit
TAGCAGCACGTAAATATTGGC101550.12985940146260946No Hit
AGCAGCATTGTACAGGGCTATGT101510.12980825054130463No Hit
CAAAGAATTCTCCTTTTGGGCT101360.12961643458641156No Hit
AACATTCAACGCTGTCGGTGAGA101230.12945019409217087No Hit
TTCAAGTAATCCAGGATAGGC101180.1293862554405399No Hit
CCCCCCACTGCTAAATTTGACTGTA100900.12902819899140613No Hit
ACTGGACTTGGAGTCAGAAGG100810.1289131094184703No Hit
AACATTCAACGCTGTCGGTGA98970.1265601670384486No Hit
CACTAGATTGTGAGCTCCTGGA94930.12139392398666188No Hit
TGAATACCGGGTGCTGTAGGCTTT94170.12042205648187033No Hit
TGTAAACATCCCCGACTGGAAGC93120.11907934479761881No Hit
ACCACAGGGTAGAACCACGGAC92650.11847832147228719No Hit
CGCGACCTCAGATCAGACGTGGCG90910.11625325639552757No Hit
GGGCTACGCCTGTCTGAGCGTCGCT89430.11436067230724926No Hit
TTATAAAGCAATGAGACTGATT88650.1133632293418053No Hit
CAAAGTGCTGTTCGTGCAGGT85710.10960363662590109No Hit
TATTGCACTTGTCCCGGCCTGTAG85530.1093734574800294No Hit
TGAGGTAGTAGGTTGTATAGT84300.1078005666499062No Hit
TGTCAGTTTGTCAAATACCCCA81370.10405376166432823No Hit
TCGTTTCCCGGCCAATGCACCA79570.10175197020561134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)