FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857810
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7025957.931926740356815No Hit
CAACGGAATCCCAAAAGCAGCTG6053706.83430780294452No Hit
AACATTCAACGCTGTCGGTGAGT2949463.3297846758961867No Hit
CCCCCACTGCTAAATTTGACTG2507212.8305077665924196No Hit
TTCAAGTAATCCAGGATAGGCT2497522.819568268003039No Hit
AAGCTGCCAGTTGAAGAACTGT2204902.489215731653761No Hit
TAGCAGCACGTAAATATTGGCG2182282.463678945472978No Hit
AACATTCAACGCTGTCGGTGAG1855472.0947277035745855No Hit
CGCCTGTCTGAGCGTCGCT1108451.2513815491639582No Hit
TAGCTTATCAGACTGATGTTGAC1097671.2392114981016753No Hit
TAGCTTATCAGACTGATGTTGA1043461.1780112691511784No Hit
GCCTGTCTGAGCGTCGCT950391.0729401511208752No Hit
TAGCTTATCAGACTGATGTTGACT827680.9344070374054084No Hit
TATTGCACTTGTCCCGGCCTGT750960.8477942064686418No Hit
TGAGGTAGTAGGTTGTATAGTT696620.786447214379175No Hit
TATTGCACTTGTCCCGGCCTGTAA655730.7402845624369906No Hit
TATTGCACTTGTCCCGGCCTGTAT631380.7127946975606837No Hit
AGCAGCATTGTACAGGGCTATGA568970.6423371013828475No Hit
CATAAAGTAGAAAGCACTACT505760.5709763474267341No Hit
TGTAAACATCCTTGACTGGAAGCT497200.56131255919917No Hit
TATTGCACTTGTCCCGGCCTGTA451400.5096067763928104No Hit
TCGTACGACTCTTAGCGG428110.4833135955727205No Hit
AACCGGGCGGAAACACCA406550.45897349344815475No Hit
TTCAAGTAATTCAGGATAGGTT404930.4571445989471438No Hit
CTCCCACTGCTTCACTTGACTAG401250.4529900731670695No Hit
ACTGGACTTGGAGTCAGAAGGC387660.43764768040858865No Hit
TGAGGTAGTAGATTGTATAGTT372860.4209392615104637No Hit
TTCTCACTACTGCACTTGACTAG324430.3662643475080184No Hit
CAACGGAATCCCAAAAGCAGCTGT304400.3436515346344074No Hit
TACGCCTGTCTGAGCGTCGCT299350.33795035115903366No Hit
ACCGGGTGCTGTAGGCTT293370.33119924676641294No Hit
CTACGCCTGTCTGAGCGTCGCT292660.33039769423819204No Hit
TGTAAACATCCCCGACTGGAAGCT284210.320858090205141No Hit
GACCCGCGGGCGCTCTCCAGTCCT275390.3109007756996368No Hit
CATTGCACTTGTCTCGGTCTGA267500.30199338211137966No Hit
ACGCCTGTCTGAGCGTCGCT262380.2962131723304067No Hit
CCCATAAAGTAGAAAGCACT261090.29475683041293504No Hit
GCGTTCGCGCTTTCCCCTG260090.29362788319008876No Hit
CTTCTCACTACTGCACTTGACTAG256520.28959754160452755No Hit
CCCATAAAGTAGAAAGCACTAC253650.2863574630749587No Hit
CAACACCAGTCGATGGGCTGT228550.2580208877815171No Hit
AACATTCAACGCTGTCGGTGAGTT224340.25326801997333426No Hit
CCCCCACTGCTAAATTTGACTGGCTT221810.2504117834995332No Hit
ACCGGGTGCTGTAGGCTTT205080.23152449646131495No Hit
TGTCAGTTTGTCAAATACCCCAA195770.22101399781661607No Hit
CCCCCCACTGCTAAATTTGACTGG180640.20393302633495186No Hit
CACCCGGCTGTGTGCACATGTGC178890.20195736869497086No Hit
AACGGAATCCCAAAAGCAGCTG171610.19373863291264998No Hit
TGTAAACATCCTACACTCTCAGCT170460.19244034360637674No Hit
ACCGGGTGCTGTAGGCTTA170120.192056501550609No Hit
TAGCAGCACGTAAATATTGGC168060.18973087027154567No Hit
GGAATACCGGGTGCTGTAGGCTTT163940.18507960771341903No Hit
ATCCCACCGCTGCCACCA163890.18502316035227667No Hit
TGAGGTAGTAGTTTGTACAGTT161120.1818959765449925No Hit
TTCACAGTGGCTAAGTTCCG159860.18047350304420617No Hit
CCCCCCACTGCTAAATTTGACTGGCT158590.17903974007119142No Hit
CTCCCACTGCTTCACTTGACTAGCCT155730.17581095101385105No Hit
CAACGGAATCCCAAAAGCAGCT151010.1704823201220166No Hit
ACCCCCCACTGCTAAATTTGACTG147870.1669374258422793No Hit
GGAATACCGGGTGCTGTAGGCTT144380.16299740003454577No Hit
AGCAGCATTGTACAGGGCTATGT139550.15754458494819826No Hit
TAGCTTATCAGACTGATGTTGACA139300.15726234814248669No Hit
TGAGGTAGTAGTTTGTGCTGTT136600.15421419064080175No Hit
TCGTTTCCCGGCCAATGCACCA135640.1531304013068693No Hit
TACCTGGTTGATCCTGCC135520.15299492764012776No Hit
ATCCCGGACGAGCCCCCA134450.15178695411168225No Hit
AGCTCGGTCTGAGGCCCCTCAGT132430.14950648072153275No Hit
TTCAAGTAATCCAGGATAGGCTT131120.14802755985960414No Hit
GCTTGACTAGCTTGCTGTTT129500.14619866535859313No Hit
TTCAAGTAATCCAGGATAGGC129160.1458148233028254No Hit
TAGCAGCACGTAAATATTGGCGT128770.14537453388591537No Hit
TGTCAGTTTGTCAAATACCCCA128150.14467458660775068No Hit
AGCAGCATTGTACAGGGCTATG126230.14250700793988583No Hit
TCCCACTGCTTCACTTGACTAG122890.13873632421557924No Hit
TATTGCACTTGTCCCGGCCTGTT122280.13804766640964303No Hit
TTAATGCTAATCGTGATAGGGGTT118870.1341979563797372No Hit
TGAGGTAGTAGGTTGTGTGGTT114930.12974990432172284No Hit
AGAATTACTCTGAGACCT114520.1292870359603559No Hit
ACCACAGGGTAGAACCACGGAC111620.1260130890141017No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109380.123484247234926No Hit
TTATAAAGCAATGAGACTGATT108490.12247948420659283No Hit
GGGCTACGCCTGTCTGAGCGTCGCT108060.12199403690076893No Hit
TGTAAACATCCTACACTCAGCT106020.11969098456616253No Hit
CTCCCACTGCTTCACTTGACTA105270.1188442741490278No Hit
ACTGGACTTGGAGTCAGAAGG103960.11736535328709918No Hit
CAAAGTGCTGTTCGTGCAGGT102830.11608964292528288No Hit
ACTGGACTTGGAGTCAGAAGGCT100370.11331243275708104No Hit
CAAAGAATTCTCCTTTTGGGCT100040.11293988017354176No Hit
CCTCGACACAAGGGTTTGT97870.11049006469996533No Hit
ACTGGACTTGGAGTCAGAAGGCA97190.10972238058842988No Hit
TTCACAGTGGCTAAGTTCCGC96250.10866117019895437No Hit
TGTAAACATCCCCGACTGGAAGC95350.10764511769839273No Hit
AACATTCAACGCTGTCGGTGA93500.10555656533612709No Hit
TGAGGTAGTAGGTTGTATAGT92650.10459696019670776No Hit
AACATTCAACGCTGTCGGTGAGA91780.1036147761128315No Hit
TTCACAGTGGCTAAGTTCTG90240.10187619738964823No Hit
TTCACAGTGGCTAAGTTCTGC89440.1009730396113712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)