FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7087527
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5364917.569509082646175No Hit
CAACGGAATCCCAAAAGCAGCTG2846114.015660187255724No Hit
AAGCTGCCAGTTGAAGAACTGT2496083.521792580119977No Hit
CCCCCACTGCTAAATTTGACTG2227013.1421538147226813No Hit
AACATTCAACGCTGTCGGTGAGT1177131.6608472884829928No Hit
ACCGGGTGCTGTAGGCTT980961.3840652741075976No Hit
AACATTCAACGCTGTCGGTGAG784231.1064931392853952No Hit
TTCAAGTAATCCAGGATAGGCT780521.1012585913252957No Hit
ACTGGACTTGGAGTCAGAAGGC777261.0966589615813809No Hit
TAGCTTATCAGACTGATGTTGACT702010.9904865265416273No Hit
TAGCTTATCAGACTGATGTTGAC659550.9305784655211896No Hit
ACCGGGTGCTGTAGGCTTT638410.9007514186542076No Hit
TATTGCACTTGTCCCGGCCTGTAA583690.8235453635661635No Hit
TAGCTTATCAGACTGATGTTGA555060.7831504557231316No Hit
TATTGCACTTGTCCCGGCCTGT519140.7324698727779096No Hit
ACCGGGTGCTGTAGGCTTA515020.7266568437763976No Hit
TATTGCACTTGTCCCGGCCTGTAT510800.7207027218379556No Hit
CGCCTGTCTGAGCGTCGCT505750.7135775285229954No Hit
TGTAAACATCCTTGACTGGAAGCT439760.620470299442951No Hit
TGTAAACATCCCCGACTGGAAGCT402770.5682800220725791No Hit
TCGTACGACTCTTAGCGG379360.5352501655372882No Hit
CAACACCAGTCGATGGGCTGT348560.4917935409628775No Hit
TATTGCACTTGTCCCGGCCTGTA304370.42944457213355236No Hit
CGCGACCTCAGATCAGACGTGG278580.3930567036993298No Hit
TGAGGTAGTAGGTTGTATAGTT253830.3581362018091783No Hit
AGCAGCATTGTACAGGGCTATGA240430.339229748260571No Hit
GGAATACCGGGTGCTGTAGGCTT236200.333261517028436No Hit
GGAATACCGGGTGCTGTAGGCTTT234610.3310181393312505No Hit
TAGCAGCACGTAAATATTGGCG229480.3237800716667464No Hit
TAATGTGAATTGCAGGACA222350.3137201452636441No Hit
CACTAGATTGTGAGCTCCTGGA214830.3031099564065153No Hit
CGCGACCTCAGATCAGACGTGGCG188820.26641168351104694No Hit
ACTGGACTTGGAGTCAGAAGGCT178170.25138528572801205No Hit
CACCCGGCTGTGTGCACATGTGC166140.23441180541534443No Hit
CAACGGAATCCCAAAAGCAGCTGT163570.2307857169362459No Hit
CGCGACCTCAGATCAGACGT159270.22471872064825996No Hit
TGAGGTAGTAGTTTGTGCTGTT156290.2205141511277488No Hit
ACTGGACTTGGAGTCAGAAGG155500.21939951692600254No Hit
GCCTGTCTGAGCGTCGCT155330.21915965893322167No Hit
CGCGACCTCAGATCAGACGTGGC154440.21790393179454554No Hit
CCCATAAAGTAGAAAGCACT150280.2120344656182615No Hit
CACTAGATTGTGAGCTCCTGGAA144310.20361121728354614No Hit
CATAAAGTAGAAAGCACTACT141360.19944897564411396No Hit
ACCACAGGGTAGAACCACGGAC135050.19054601132383692No Hit
TACGCCTGTCTGAGCGTCGCT128650.181516063360323No Hit
CTACGCCTGTCTGAGCGTCGCT122220.17244378751573008No Hit
CGCGACCTCAGATCAGACGTG118870.16771717412857828No Hit
CATTGCACTTGTCTCGGTCTGA115040.1623133146441629No Hit
CCGGGTGCTGTAGGCTTT111820.15777012207501995No Hit
TGAGGTAGTAGGTTGTGTGGTT108360.15288830645724524No Hit
AACGAACGAGACTCTGGCATG107890.1522251696536747No Hit
AACGAACGAGACTCTGGCATGCT103820.14648268712062756No Hit
CCCCCCACTGCTAAATTTGACTGG98710.1392728380435094No Hit
TATTGCACTTGTCCCGGCCTGTAG97490.13755150421296455No Hit
CCCATAAAGTAGAAAGCACTAC97030.136902476703087No Hit
TGTAAACATCCCCGACTGGAAGC96370.1359712633193496No Hit
CTGGACTTGGAGTCAGAAGGC95600.13488484770498935No Hit
ACTGGACTTGGAGTCAGAAGGCA93950.13255681424564592No Hit
CAACGGAATCCCAAAAGCAGCT90420.12757623357202025No Hit
TCCACCACGTTCCCGTGG89780.12667323877566886No Hit
CAAAGAATTCTCCTTTTGGGCT89060.12565736962977353No Hit
CGCGACCTCAGATCAGAC87790.12386548933076376No Hit
GACCCGCGGGCGCTCTCCAGTCCT87530.123498647694746No Hit
AACGGAATCCCAAAAGCAGCTG87110.1229060573596404No Hit
CCCCCCACTGCTAAATTTGACTGT85900.12119883282278854No Hit
GGGAATACCGGGTGCTGTAGGCTT84990.11991488709672639No Hit
TACCACCCTGAACGCGCCCGA84900.1197879034534895No Hit
ATACCGGGTGCTGTAGGCTT84410.11909654806253295No Hit
AACGAGACTCTGGCATGCT84240.11885669006975212No Hit
AGCTCGGTCTGAGGCCCCTCAGT82680.1166556402536456No Hit
TATTGCACTTGTCCCGGCCTGTT79530.11221121274035359No Hit
CGCGACCTCAGATCAGACG79130.11164684099263396No Hit
ACGCCTGTCTGAGCGTCGCT78270.11043344173503677No Hit
GAATACCGGGTGCTGTAGGCTTT75810.10696255548656111No Hit
CCCCCCACTGCTAAATTTGACTGTA75730.10684968113701719No Hit
GAATACCGGGTGCTGTAGGCTT74420.10500136366323543No Hit
TTCACAGTGGCTAAGTTCTG73600.10384440158041021No Hit
ACGAGACTCTGGCATGCT73410.10357632500024337No Hit
TTCTCACTACTGCACTTGACTAG72840.10277209525974293No Hit
GCGTTCGCGCTTTCCCCTG72650.1025040186795761No Hit
ACGGACCAGAGCGAAAGCATT72370.10210895845617236No Hit
CCCCCACTGCTAAATTTGACTGGCTT72240.1019255376381635No Hit
ATCCCACCGCTGCCACCA71380.10071213838056632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)