FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8953515
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7566578.450949152372003No Hit
CAACGGAATCCCAAAAGCAGCTG5430316.065003520963554No Hit
AAGCTGCCAGTTGAAGAACTGT3244613.62383935247777No Hit
TTCAAGTAATCCAGGATAGGCT3134443.5007927054346815No Hit
AACATTCAACGCTGTCGGTGAGT3079043.4389175647776318No Hit
CCCCCACTGCTAAATTTGACTG2769843.09357833208522No Hit
TAGCAGCACGTAAATATTGGCG2539242.8360258512997407No Hit
AACATTCAACGCTGTCGGTGAG1836432.0510715623975613No Hit
TAGCTTATCAGACTGATGTTGA1190131.329232150725162No Hit
TATTGCACTTGTCCCGGCCTGT1109221.2388654064911937No Hit
TAGCTTATCAGACTGATGTTGACT1087931.2150870356502446No Hit
TGAGGTAGTAGGTTGTATAGTT1086051.2129873016351678No Hit
TATTGCACTTGTCCCGGCCTGTAT1075811.201550452531771No Hit
TATTGCACTTGTCCCGGCCTGTAA1057351.1809328515113897No Hit
TAGCTTATCAGACTGATGTTGAC1052531.1755494908982673No Hit
CATAAAGTAGAAAGCACTACT905011.0107873835024568No Hit
TGAGGTAGTAGATTGTATAGTT689230.7697870612826359No Hit
TATTGCACTTGTCCCGGCCTGTA611900.6834187467156754No Hit
CGCCTGTCTGAGCGTCGCT587340.6559881789442471No Hit
TGTAAACATCCTTGACTGGAAGCT562210.6279209896895243No Hit
TTCAAGTAATTCAGGATAGGTT473140.5284405063262864No Hit
ACTGGACTTGGAGTCAGAAGGC446200.4983517646421545No Hit
CCCATAAAGTAGAAAGCACTAC435760.486691539579707No Hit
AGCAGCATTGTACAGGGCTATGA413160.46145005620697566No Hit
ATCCCGGACGAGCCCCCA376490.4204940741150264No Hit
TTCTCACTACTGCACTTGACTAG371820.4152782454711921No Hit
CCCATAAAGTAGAAAGCACT342700.3827547058334073No Hit
ACCGGGTGCTGTAGGCTT340900.38074432220195087No Hit
TGTAAACATCCCCGACTGGAAGCT338140.3776617339670509No Hit
CATTGCACTTGTCTCGGTCTGA328380.36676098716537586No Hit
CAACGGAATCCCAAAAGCAGCTGT320140.3575578976524862No Hit
ACCGGGTGCTGTAGGCTTA307610.34356339381795864No Hit
TGAGGTAGTAGTTTGTACAGTT293670.32799408947212355No Hit
TTAATGCTAATCGTGATAGGGGTT291740.32583851146728404No Hit
CAACACCAGTCGATGGGCTGT281850.314792570292226No Hit
CTCCCACTGCTTCACTTGACTAG270450.302060140626335No Hit
TCGTACGACTCTTAGCGG259880.29025472119050455No Hit
ACCGGGTGCTGTAGGCTTT236160.2637623324470892No Hit
CTACGCCTGTCTGAGCGTCGCT220710.24650653961042118No Hit
CTTCTCACTACTGCACTTGACTAG204280.22815620457440458No Hit
TGTAAACATCCTACACTCTCAGCT197800.22091882350116127No Hit
CGCGACCTCAGATCAGACGTGGCG196950.21996947567519573No Hit
CGCGACCTCAGATCAGACGTGG195600.21846168795160337No Hit
TTCACAGTGGCTAAGTTCCG188010.2099845703056286No Hit
TATTGCACTTGTCCCGGCCTGTT186430.2082199002291279No Hit
TGAGGTAGTAGTTTGTGCTGTT185120.20675678769734568No Hit
TTCACAGTGGCTAAGTTCCGC176820.19748668539674086No Hit
CCCCCCACTGCTAAATTTGACTGG174000.1943370843741257No Hit
AACATTCAACGCTGTCGGTGAGTT173990.19432591557617315No Hit
TACGCCTGTCTGAGCGTCGCT172000.19210332478361852No Hit
TGTCAGTTTGTCAAATACCCCAA171490.19153371608803918No Hit
ACGCCTGTCTGAGCGTCGCT163430.1825316649382952No Hit
TAGCAGCACATCATGGTTTACA162590.18159348591028215No Hit
ACCCCCCACTGCTAAATTTGACTG162260.18122491557784848No Hit
GACCCGCGGGCGCTCTCCAGTCCT161600.1804877749129811No Hit
TTCAAGTAATCCAGGATAGGC159480.1781199897470435No Hit
TAGCAGCACGTAAATATTGGC157790.1762324628930649No Hit
CACTAGATTGTGAGCTCCTGGA156450.1747358439674251No Hit
AACGGAATCCCAAAAGCAGCTG149710.16720807414741584No Hit
CAAAGAATTCTCCTTTTGGGCT145050.1620034143015341No Hit
TTCACAGTGGCTAAGTTCTGC141100.15759173911028238No Hit
ATCCCACCGCTGCCACCA136980.15299019435383757No Hit
CCCCCACTGCTAAATTTGACTGGCTT136460.1524094168603057No Hit
TGAGGTAGTAGGTTGTGTGGTT134500.15022033246160865No Hit
TCAGGCTCAGTCCCCTCCCGAT134360.15006396929027316No Hit
TTCAAGTAATCCAGGATAGGCTT133640.14925981583769057No Hit
CAAAGTGCTGTTCGTGCAGGT132550.14804241686086414No Hit
TGAGAACTGAATTCCATGGGTT126970.14181022760334908No Hit
TGAGGTAGTAGGTTGTATAGT126130.14087204857533606No Hit
TGTCAGTTTGTCAAATACCCCA125820.14052581583880744No Hit
TCAGGCTCAGTCCCCTCCCGA125290.13993386954732304No Hit
TGTAAACATCCTACACTCAGCT123380.13780062913838867No Hit
ACTGGACTTGGAGTCAGAAGGCT122750.1370969948673789No Hit
TTCACAGTGGCTAAGTTCTG122070.13633751660660645No Hit
ACTGGACTTGGAGTCAGAAGG121030.1351759616195427No Hit
TTATAAAGCAATGAGACTGATT120920.13505310484206481No Hit
CGCGACCTCAGATCAGACGT120440.1345170025403431No Hit
TATTGCACTTGTCCCGGCCTGTAG119730.13372401788571303No Hit
CCCCCCACTGCTAAATTTGACTGT119330.1332772659676116No Hit
AGCTCGGTCTGAGGCCCCTCAGT114440.12781572376882153No Hit
GTCAGGATGGCCGAGCGGTCTAAGG113940.12725728387119473No Hit
CGCGACCTCAGATCAGACGTGGC112170.12528040663359585No Hit
TAGCTTATCAGACTGATGTTGACA110630.12356041174890532No Hit
CACTAGATTGTGAGCTCCTGGAA110110.12297963425537345No Hit
GCCTGTCTGAGCGTCGCT109530.12233184397412637No Hit
ACTGGACTTGGAGTCAGAAGGCA109310.12208613041917056No Hit
TGTAAACATCCCCGACTGGAAGC107560.12013159077747677No Hit
AACATTCAACGCTGTCGGTGA106650.11911523016379601No Hit
CCCCCCACTGCTAAATTTGACTGTA105680.11803185676240002No Hit
ACCACAGGGTAGAACCACGGAC105340.1176521176320138No Hit
CACCCGGCTGTGTGCACATGTGC103880.11602147313094355No Hit
CCCCCCACTGCTAAATTTGACTGGCT98800.11034772377105527No Hit
AGCAGCATTGTACAGGGCTATGT98690.11022486699357739No Hit
GGAATACCGGGTGCTGTAGGCTTT98340.10983395906523861No Hit
TCCCACTGCTTCACTTGACTAG97730.10915266239013392No Hit
TAGCAGCACGTAAATATTGGCGT97400.10878409205770025No Hit
GGAATACCGGGTGCTGTAGGCTT96590.10787941942354484No Hit
TACCACCCTGAACGCGCCCGA94520.10556747824736988No Hit
CGCGACCTCAGATCAGACGTG94410.105444621469892No Hit
AATGACACGATCACTCCCGTTG94290.10531059589446157No Hit
TATTGCACTTGTCCCGGCCTGTTT93740.10469631200707209No Hit
CAAAGAATTCTCCTTTTGGGCTT93360.10427189768487573No Hit
CCCATAAAGTAGAAAGCACTA93220.10411553451354022No Hit
TGATGTAGTAGGTTGTATAGTT91750.10247372121451742No Hit
TATTGCACTTGTCCCGGCCTGA90730.10133450382335876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)