FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7393746
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG81512711.024546961716023No Hit
CAACGGAATCCCAAAAGCAGCTG6117868.274371340319238No Hit
CCCCCACTGCTAAATTTGACTG2853773.8597079207211067No Hit
AACATTCAACGCTGTCGGTGAGT2678453.6225886039363537No Hit
AAGCTGCCAGTTGAAGAACTGT2109062.8524918221426594No Hit
AACATTCAACGCTGTCGGTGAG1990322.6918966380505904No Hit
TAGCAGCACGTAAATATTGGCG1863192.5199540260106312No Hit
TTCAAGTAATCCAGGATAGGCT1737262.3496344072409303No Hit
TAGCTTATCAGACTGATGTTGACT946351.2799330677575347No Hit
TATTGCACTTGTCCCGGCCTGTAA928291.2555070190401454No Hit
GGAATACCGGGTGCTGTAGGCTTT882151.19310292779871No Hit
TAGCTTATCAGACTGATGTTGAC857351.1595610668800362No Hit
TATTGCACTTGTCCCGGCCTGTAT817451.1055965406439443No Hit
TAGCTTATCAGACTGATGTTGA785021.0617351475152108No Hit
GGAATACCGGGTGCTGTAGGCTT683440.9243487671878369No Hit
TGAGGTAGTAGGTTGTATAGTT675620.9137722610433197No Hit
TATTGCACTTGTCCCGGCCTGT670240.9064958412149944No Hit
CGCCTGTCTGAGCGTCGCT565320.7645921296187346No Hit
GCCTGTCTGAGCGTCGCT483550.653998663194543No Hit
TATTGCACTTGTCCCGGCCTGTA439970.5950569575963253No Hit
TGTAAACATCCTTGACTGGAAGCT407350.5509385905331343No Hit
TGAGGTAGTAGATTGTATAGTT385620.5215488873975385No Hit
CATAAAGTAGAAAGCACTACT378430.5118244527199068No Hit
ACTGGACTTGGAGTCAGAAGGC361980.48957591997344785No Hit
TTAATGCTAATCGTGATAGGGGTT361410.4888049981700751No Hit
AGCAGCATTGTACAGGGCTATGA346210.4682470834134686No Hit
CAACGGAATCCCAAAAGCAGCTGT294410.3981878739139808No Hit
TTCAAGTAATTCAGGATAGGTT281260.38040257266073246No Hit
TGAATACCGGGTGCTGTAGGCTTT279670.3782521065776401No Hit
TGTAAACATCCCCGACTGGAAGCT274840.37171955866485No Hit
TCGTACGACTCTTAGCGG245080.3314693255624415No Hit
CCCATAAAGTAGAAAGCACT223050.30167387410928104No Hit
TGAATACCGGGTGCTGTAGGCTT218620.29568232395324373No Hit
CAACACCAGTCGATGGGCTGT212970.2880407306391104No Hit
CAACGGAATCCCAAAAGCAGCT211640.2862419130979073No Hit
CCCATAAAGTAGAAAGCACTAC210490.284686544547243No Hit
CATTGCACTTGTCTCGGTCTGA202870.2743805372811022No Hit
TTCTCACTACTGCACTTGACTAG178230.24105507546512958No Hit
CTACGCCTGTCTGAGCGTCGCT176290.2384312363448785No Hit
TACGCCTGTCTGAGCGTCGCT172790.23369750597329148No Hit
TGAGGTAGTAGTTTGTACAGTT168970.22853097739630224No Hit
ACCGGGTGCTGTAGGCTT163870.221633255997704No Hit
TCCTGTCTGAGCGTCGCT157770.21338304020722376No Hit
AACATTCAACGCTGTCGGTGAGTT156560.21174652199304655No Hit
ACGCCTGTCTGAGCGTCGCT155450.21024525321805757No Hit
CGCGACCTCAGATCAGAC153320.20736444016334885No Hit
AACGGAATCCCAAAAGCAGCTG145800.19719368233639617No Hit
GGAATACCGGGTGCTGTAGGCTTA139450.18860534294794548No Hit
ACCGGGTGCTGTAGGCTTA125890.17026551899402548No Hit
CACTAGATTGTGAGCTCCTGGA124870.16888597471430583No Hit
ACCCCCCACTGCTAAATTTGACTG124460.16833145201363423No Hit
ACTGGACTTGGAGTCAGAAGGCT121740.16465266726771519No Hit
TATTGCACTTGTCCCGGCCTGTT119520.16165012971773712No Hit
TAGCAGCACGTAAATATTGGC117760.1592697395880248No Hit
GACCCGCGGGCGCTCTCCAGTCCT117350.15871521688735318No Hit
TGTCAGTTTGTCAAATACCCCAA115840.15667295035561135No Hit
TGAGGTAGTAGTTTGTGCTGTT114930.15544218045899874No Hit
TATTGCACTTGTCCCGGCCTGTAG113610.1536568878617145No Hit
ACCGGGTGCTGTAGGCTTT110280.14915308153674742No Hit
ACTGGACTTGGAGTCAGAAGGCA109530.1481387107428359No Hit
CCCCCCACTGCTAAATTTGACTGG107990.14605586937933762No Hit
CTTCTCACTACTGCACTTGACTAG107360.14520379791245194No Hit
TGTAAACATCCTACACTCTCAGCT106950.14464927521178034No Hit
TCGTTTCCCGGCCAATGCACCA106670.14427057678205338No Hit
CACTAGATTGTGAGCTCCTGGAA104230.1409704904658613No Hit
TAGCTTATCAGACTGATGTTGACA103750.14032129315775793No Hit
CACCCGGCTGTGTGCACATGTGC101900.13781917853277623No Hit
CCCCCACTGCTAAATTTGACTGGCTT100090.1353711636834698No Hit
CAAAGAATTCTCCTTTTGGGCT99780.13495189042198635No Hit
ATCCCGGACGAGCCCCCA95820.12959601263013362No Hit
CTCCCACTGCTTCACTTGACTAG95580.12927141397608194No Hit
CGCGACCTCAGATCAGACGTGGCG95400.1290279649855432No Hit
GGAATACCGGGTGCTGTAGGCT94330.12758079598622943No Hit
TTCAAGTAATCCAGGATAGGCTT93830.12690454879028845No Hit
CGCGACCTCAGATCAGACGTGG92400.12497048180989717No Hit
GCGTTCGCGCTTTCCCCTG91550.12382086157679748No Hit
TGAGGTAGTAGGTTGTGTGGTT89990.12171097032546155No Hit
AACCGGGCGGAAACACCA86300.11672026601941696No Hit
TTCACAGTGGCTAAGTTCTG85800.11604401882347595No Hit
TTCAAGTAATCCAGGATAGGC84560.11436692577754227No Hit
ACCACAGGGTAGAACCACGGAC83770.11329845520795549No Hit
ACTGGACTTGGAGTCAGAAGG82200.11117503901270075No Hit
TGAGAACTGAATTCCATGGGTT81100.10968729518163053No Hit
AGCAGCATTGTACAGGGCTATGT78560.10625195942625024No Hit
TTCACAGTGGCTAAGTTCCG77340.10460191626815418No Hit
TTCACAGTGGCTAAGTTCTGC76820.10389861918437555No Hit
CCCCCCACTGCTAAATTTGACTGT76280.10316827221275926No Hit
TTATAAAGCAATGAGACTGATT75890.10264079939992529No Hit
TGTAAACATCCCCGACTGGAAGC75510.10212685153101013No Hit
CGCCCCACTGCTAAATTTGACTG75500.1021133265870913No Hit
AACATTCAACGCTGTCGGTGA75410.10199160209182193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)