FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7404364
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG94376112.746010325802459No Hit
CAACGGAATCCCAAAAGCAGCTG5602257.566146126797656No Hit
CCCCCACTGCTAAATTTGACTG3257004.398757273413355No Hit
AACATTCAACGCTGTCGGTGAGT2686283.6279685871737266No Hit
AAGCTGCCAGTTGAAGAACTGT2164602.9234111126897595No Hit
TAGCAGCACGTAAATATTGGCG2036092.749851303906723No Hit
AACATTCAACGCTGTCGGTGAG1988292.6852947802133986No Hit
TTCAAGTAATCCAGGATAGGCT1908112.577007289214847No Hit
TAGCTTATCAGACTGATGTTGACT1009091.3628314329225306No Hit
TAGCTTATCAGACTGATGTTGA985061.3303775989403006No Hit
TAGCTTATCAGACTGATGTTGAC968401.3078773544898659No Hit
TATTGCACTTGTCCCGGCCTGTAA808321.0916805278616772No Hit
TGAGGTAGTAGGTTGTATAGTT737200.9956290641572998No Hit
TATTGCACTTGTCCCGGCCTGTAT713880.9641341241462468No Hit
TATTGCACTTGTCCCGGCCTGT684580.9245628658990833No Hit
CGCCTGTCTGAGCGTCGCT585030.7901151267009564No Hit
TATTGCACTTGTCCCGGCCTGTA499440.6745211337530138No Hit
TGTAAACATCCTTGACTGGAAGCT447920.6049405458726773No Hit
CATAAAGTAGAAAGCACTACT409850.5535249212491444No Hit
TTAATGCTAATCGTGATAGGGGTT378870.5116847307884917No Hit
AGCAGCATTGTACAGGGCTATGA376240.50813277143047No Hit
ACTGGACTTGGAGTCAGAAGGC355410.4800007130929814No Hit
TGAGGTAGTAGATTGTATAGTT353670.4776507475861533No Hit
TGTAAACATCCCCGACTGGAAGCT302670.40877244824808723No Hit
TTCAAGTAATTCAGGATAGGTT266560.36000391120695846No Hit
CAACGGAATCCCAAAAGCAGCTGT251870.3401642598878175No Hit
CAACACCAGTCGATGGGCTGT247470.3342218183762981No Hit
GCCTGTCTGAGCGTCGCT239110.32293117950441114No Hit
CATTGCACTTGTCTCGGTCTGA235100.3175154543995946No Hit
ACCGGGTGCTGTAGGCTT222620.3006605293851031No Hit
CAACGGAATCCCAAAAGCAGCT216440.2923141001711963No Hit
TCGTACGACTCTTAGCGG213570.28843800763981886No Hit
TTCTCACTACTGCACTTGACTAG206390.27874102353693037No Hit
CCCATAAAGTAGAAAGCACT203880.27535113076558637No Hit
AACATTCAACGCTGTCGGTGAGTT176610.23852149894305574No Hit
CCCATAAAGTAGAAAGCACTAC175130.23652267770736282No Hit
GGAATACCGGGTGCTGTAGGCTTT166000.22419211157096003No Hit
CTACGCCTGTCTGAGCGTCGCT155740.21033541841000794No Hit
ACCGGGTGCTGTAGGCTTA151630.2047846378162932No Hit
TACGCCTGTCTGAGCGTCGCT149120.20139474504494917No Hit
CACTAGATTGTGAGCTCCTGGA146990.1985180631314182No Hit
ACCGGGTGCTGTAGGCTTT145860.19699193610686885No Hit
TGAGGTAGTAGTTTGTACAGTT145850.19697843055797906No Hit
GGAATACCGGGTGCTGTAGGCTT144390.1950066204200658No Hit
ACGCCTGTCTGAGCGTCGCT135560.1830812207503575No Hit
TGATGTAGTAGGTTGTATAGTT135530.18304070410368803No Hit
CCCCCCACTGCTAAATTTGACTGG130540.17630143520766942No Hit
TATTGCACTTGTCCCGGCCTGTT128240.17319515896301155No Hit
TTCACAGTGGCTAAGTTCTG127120.17168253748735207No Hit
CTTCTCACTACTGCACTTGACTAG126960.171466448705115No Hit
TTCACAGTGGCTAAGTTCCG126830.17129087656954736No Hit
CAAAGAATTCTCCTTTTGGGCT126590.17096674339619175No Hit
AACGGAATCCCAAAAGCAGCTG125810.16991331058278603No Hit
TAGCAGCACGTAAATATTGGC125020.1688463722204905No Hit
ACCCCCCACTGCTAAATTTGACTG124270.16783345605375424No Hit
CGCGACCTCAGATCAGACGTGG124000.1674688062337292No Hit
GACCCGCGGGCGCTCTCCAGTCCT115550.15605661742183394No Hit
TAGCTTATCAGACTGATGTTGACA114830.1550842179017671No Hit
CGCGACCTCAGATCAGACGTGGCG114020.15399026844169195No Hit
ACTGGACTTGGAGTCAGAAGGCT113530.15332849654609093No Hit
TGTCAGTTTGTCAAATACCCCAA112660.15215351379267686No Hit
TGTAAACATCCTACACTCTCAGCT112400.1518023695215416No Hit
CACTAGATTGTGAGCTCCTGGAA111400.15045181463255994No Hit
ACTGGACTTGGAGTCAGAAGGCA111240.15023572585032285No Hit
ATCCCGGACGAGCCCCCA109510.14789926589238456No Hit
TGAGGTAGTAGTTTGTGCTGTT109410.1477642104034864No Hit
ACTGGACTTGGAGTCAGAAGG106050.1432263459765079No Hit
TATTGCACTTGTCCCGGCCTGTAG104760.14148413016972153No Hit
CCCCCACTGCTAAATTTGACTGGCTT102950.13903962582066468No Hit
CTCCCACTGCTTCACTTGACTAG99700.13465032243147418No Hit
TCCTGTCTGAGCGTCGCT97740.1320032348490701No Hit
TCGTTTCCCGGCCAATGCACCA95400.12884293640885294No Hit
TTCAAGTAATCCAGGATAGGC92520.12495333832858568No Hit
TGAGGTAGTAGGTTGTGTGGTT92110.12439961082410318No Hit
AGCAGCATTGTACAGGGCTATG90070.12164447885058055No Hit
GCGTTCGCGCTTTCCCCTG88460.11947008547932003No Hit
TGAGAACTGAATTCCATGGGTT88040.11890285242594771No Hit
ACCACAGGGTAGAACCACGGAC85430.11537790416570551No Hit
TTCAAGTAATCCAGGATAGGCTT85430.11537790416570551No Hit
AGCAGCATTGTACAGGGCTATGT83480.11274432213219122No Hit
CCCCCCACTGCTAAATTTGACTGT81820.11050240101648162No Hit
TGTAAACATCCCCGACTGGAAGC81520.11009723454978712No Hit
CAAAGTGCTGTTCGTGCAGGT80100.10817944660743313No Hit
TTATAAAGCAATGAGACTGATT78670.10624815311618932No Hit
CACCCGGCTGTGTGCACATGTGC78580.10612660317618097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)