FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7198020
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103607814.393930553124331No Hit
CAACGGAATCCCAAAAGCAGCTG5501457.64300460404389No Hit
CCCCCACTGCTAAATTTGACTG3862075.365461613054701No Hit
TAGCAGCACGTAAATATTGGCG2384413.3125915182230665No Hit
AACATTCAACGCTGTCGGTGAGT2337553.2474902820497857No Hit
AAGCTGCCAGTTGAAGAACTGT2104202.9233039085748582No Hit
TTCAAGTAATCCAGGATAGGCT1971222.7385586591868316No Hit
AACATTCAACGCTGTCGGTGAG1662472.309621256956774No Hit
TAGCTTATCAGACTGATGTTGA1155731.6056221016335046No Hit
TAGCTTATCAGACTGATGTTGAC1091751.5167365469948682No Hit
TAGCTTATCAGACTGATGTTGACT1078191.4978980330702054No Hit
CGCCTGTCTGAGCGTCGCT684750.9513032750673102No Hit
TGAGGTAGTAGGTTGTATAGTT681370.9466075392955285No Hit
TATTGCACTTGTCCCGGCCTGT561600.780214559003726No Hit
TATTGCACTTGTCCCGGCCTGTAA516800.7179752209635427No Hit
CATAAAGTAGAAAGCACTACT502660.6983309298946099No Hit
TGTAAACATCCTTGACTGGAAGCT480160.6670723337806785No Hit
TATTGCACTTGTCCCGGCCTGTAT439330.6103484013659312No Hit
AGCAGCATTGTACAGGGCTATGA344050.47797866635547004No Hit
ACTGGACTTGGAGTCAGAAGGC338720.4705738522538142No Hit
TGAGGTAGTAGATTGTATAGTT306070.42521415611515384No Hit
TATTGCACTTGTCCCGGCCTGTA303850.42212997463191265No Hit
CAACACCAGTCGATGGGCTGT282450.39239957655021795No Hit
TGTAAACATCCCCGACTGGAAGCT278460.38685638550601414No Hit
CATTGCACTTGTCTCGGTCTGA265510.36886532685377366No Hit
TTCAAGTAATTCAGGATAGGTT257950.35836243855949274No Hit
TCGTACGACTCTTAGCGG252600.3509298390390691No Hit
CAACGGAATCCCAAAAGCAGCTGT242340.3366759192111164No Hit
CCCATAAAGTAGAAAGCACT241330.33527275556333547No Hit
ACCGGGTGCTGTAGGCTT234200.3253672537725652No Hit
CAACGGAATCCCAAAAGCAGCT233230.3240196609623202No Hit
CCCATAAAGTAGAAAGCACTAC222540.30916835463085685No Hit
TTCTCACTACTGCACTTGACTAG180780.2511524002434003No Hit
CTACGCCTGTCTGAGCGTCGCT179500.24937413344225218No Hit
TACGCCTGTCTGAGCGTCGCT173490.24102461510248654No Hit
TTCACAGTGGCTAAGTTCCG159120.2210607917177224No Hit
AACATTCAACGCTGTCGGTGAGTT145030.20148596419570938No Hit
ACGCCTGTCTGAGCGTCGCT144610.20090247040158266No Hit
CCCCCACTGCTAAATTTGACTGGCTT139870.19431732615358113No Hit
TAGCAGCACGTAAATATTGGC136750.18998280082578264No Hit
AACGGAATCCCAAAAGCAGCTG134250.18650962347979028No Hit
TGAGGTAGTAGTTTGTACAGTT133670.18570384633552003No Hit
GACCCGCGGGCGCTCTCCAGTCCT131300.1824112742115193No Hit
ATCCCGGACGAGCCCCCA131130.1821750981519918No Hit
TAGCTTATCAGACTGATGTTGACA130370.18111925223881012No Hit
TTCACAGTGGCTAAGTTCTG129300.17963273233472538No Hit
CACTAGATTGTGAGCTCCTGGA128480.1784935301652399No Hit
TGTCAGTTTGTCAAATACCCCAA127770.17750714779897805No Hit
CGCGACCTCAGATCAGACGTGGCG124190.172533557839517No Hit
ACCGGGTGCTGTAGGCTTT122790.17058857852576126No Hit
ACCCCCCACTGCTAAATTTGACTG117860.1637394727994643No Hit
CAAAGAATTCTCCTTTTGGGCT115660.160683076734991No Hit
TGTAAACATCCTACACTCTCAGCT112670.15652915662918415No Hit
ACCGGGTGCTGTAGGCTTA108930.15133328331957957No Hit
TGAGGTAGTAGTTTGTGCTGTT108720.1510415364225162No Hit
CCCCCCACTGCTAAATTTGACTGG107820.14979119257795895No Hit
TTCAAGTAATCCAGGATAGGC106600.14809628203311467No Hit
ACTGGACTTGGAGTCAGAAGGCT104180.14473424636219404No Hit
CTTCTCACTACTGCACTTGACTAG103100.14323383374872534No Hit
ACTGGACTTGGAGTCAGAAGGCA100940.14023300852178794No Hit
TTCACAGTGGCTAAGTTCTGC100130.1391076990616864No Hit
CTCCCACTGCTTCACTTGACTAG100030.1389687719678467No Hit
TATTGCACTTGTCCCGGCCTGTT99990.13891320113031083No Hit
GCGTTCGCGCTTTCCCCTG97560.13553727275000624No Hit
TCGTTTCCCGGCCAATGCACCA96710.13435639245236883No Hit
CACTAGATTGTGAGCTCCTGGAA95720.13298101422335587No Hit
ACTGGACTTGGAGTCAGAAGG94620.13145281619111923No Hit
CGCGACCTCAGATCAGACGTGG92950.12913273372399633No Hit
TTCAAGTAATCCAGGATAGGCTT87830.12201966651940394No Hit
CAAAGTGCTGTTCGTGCAGGT86890.12071375183731081No Hit
TGTAAACATCCCCGACTGGAAGC85920.11936615902706578No Hit
TGAGGTAGTAGGTTGTGTGGTT85370.11860206001094746No Hit
TTCACAGTGGCTAAGTTCCGC84170.11693493488487111No Hit
TTATAAAGCAATGAGACTGATT83790.11640701192828028No Hit
CTCCCACTGCTTCACTTGACTAGCCT83380.11583741084353752No Hit
AGCAGCATTGTACAGGGCTATG82520.11464263783651615No Hit
ACCACAGGGTAGAACCACGGAC81640.11342007941072683No Hit
CACCCGGCTGTGTGCACATGTGC80850.11232255536939326No Hit
GCCTGTCTGAGCGTCGCT80150.11135006571251539No Hit
TGTCAGTTTGTCAAATACCCCA78790.10946065723629553No Hit
TAGCAGCACGTAAATATTGGCGT78370.10887716344216883No Hit
AGCAGCATTGTACAGGGCTATGT75960.10552902048063219No Hit
GGAATACCGGGTGCTGTAGGCTTT73650.10231980461293522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)