FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752891
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG77697610.021758335052047No Hit
TCGTACGACTCTTAGCGG4438525.724986975826178No Hit
TGAGGTAGTAGATTGTATAGTT3863124.982812218048725No Hit
CCCCCCACTGCTAAATTTGACTG3266284.212983260050993No Hit
TTCAAGTAATCCAGGATAGGCT3063133.950951973915279No Hit
GCCTGTCTGAGCGTCGCT2414963.1149154554088274No Hit
TAGCTTATCAGACTGATGTTGA2023342.609787755303151No Hit
TAGCAGCACGTAAATATTGGCG1933972.494514626866288No Hit
CCCCCACTGCTAAATTTGACTG1691182.181354026517334No Hit
TAGCTTATCAGACTGATGTTGAC1642012.117932523493494No Hit
TGAGGTAGTAGGTTGTATAGTT1533951.9785522587638598No Hit
AAGCTGCCAGTTGAAGAACTGT1413511.8232037571532995No Hit
TTCAAGTAATTCAGGATAGGTT1212531.5639714269167462No Hit
AACATTCAACGCTGTCGGTGAG1156521.4917274085241234No Hit
TGAGGTAGTAGTTTGTACAGTT1151601.485381388697455No Hit
TAGCTTATCAGACTGATGTTGACT927691.1965729945126276No Hit
CATAAAGTAGAAAGCACTACT927171.1959022769699714No Hit
TATTGCACTTGTCCCGGCCTGT799141.0307638789194895No Hit
AACCGGGCGGAAACACCA658870.8498378217880272No Hit
TGATGTAGTAGATTGTATAGTT576670.7438128563912482No Hit
TATTGCACTTGTCCCGGCCTGTAT567680.732217181951868No Hit
TATTGCACTTGTCCCGGCCTGTAA549140.7083035218733243No Hit
TGAGAACTGAATTCCATGGGTT530290.6839900109520436No Hit
TTCTCACTACTGCACTTGACTAG511870.6602311318448821No Hit
TGTAAACATCCTTGACTGGAAGCT485620.6263727943550348No Hit
TGAGGTAGTAGATTGTATAGT475290.6130487324018872No Hit
TTCACAGTGGCTAAGTTCCGC470920.6074121253607203No Hit
CATTGCACTTGTCTCGGTCTGA435710.5619968086743383No Hit
TATTGCACTTGTCCCGGCCTGTA353090.45543010987772176No Hit
AACATTCAACGCTGTCGGTGAGT351070.4528246301927887No Hit
CAACGGAATCCCAAAAGCAGCTGT329910.42553158557240134No Hit
CAACACCAGTCGATGGGCTGT301100.38837125402640127No Hit
AGCAGCATTGTACAGGGCTATGA286170.3691139215036043No Hit
TGAGGTAGTAAGTTGTATTGTT260640.33618426984204985No Hit
CACCCGGCTGTGTGCACATGTGC260500.33600369204210406No Hit
TCGTTTCCCGGCCAATGCACCA245260.3163465086765698No Hit
CTCCCACTGCTTCACTTGACTAG245140.31619172770518766No Hit
CAACGGAATCCCAAAAGCAGCT244640.31554680699109533No Hit
CTTCTCACTACTGCACTTGACTAG242060.3122190161063789No Hit
TGAGGTAGTAGTTTGTGCTGTT239180.3085042727932071No Hit
AACGGAATCCCAAAAGCAGCTG230950.29788887783924733No Hit
TGATGTAGTAGGTTGTATAGTT229140.2955542648542331No Hit
AGGTCCCTGTTCGGGCGCCA218690.28207542192970336No Hit
TTATAAAGCAATGAGACTGATT191000.24635971278327015No Hit
TGAGAACTGAATTCCATAGGCTGT183390.23654401951478488No Hit
TGGCTCAGTTCAGCAGGAAC178440.23015930444527083No Hit
TTCACAGTGGCTAAGTTCTGC177860.22941119641692367No Hit
TAGCAGCACGTAAATATTGGC176630.22782469146025658No Hit
GGAATACCGGGTGCTGTAGGCTTT176460.22760541841746518No Hit
TGTAAACATCCCCGACTGGAAGCT171180.22079505567665017No Hit
TGTAAACATCCCCGACTGGA169340.2184217474487904No Hit
TGATGTAGTAGTTTGTACAGTT167680.21628061067800386No Hit
TGTCAGTTTGTCAAATACCCCA157880.2036401646817942No Hit
ACTGGACTTGGAGTCAGAAGGC156910.20238901849645505No Hit
TAGCTTATCAGACTGATGTTG150420.1940179476275366No Hit
TAGCTTATCAGACTGATGTTGACA147240.18991625188590938No Hit
CCCCCACTGCTAAATTTGACTGGCTT147220.18989045505734573No Hit
TCAGTGCACTACAGAACTTTGT137910.17788203136094652No Hit
GGAATACCGGGTGCTGTAGGCTT137010.1767211740755803No Hit
TGAGGTAGTAGATTGTATAGTTA135040.17418018646205652No Hit
TTCAAGTAATCCAGGATAGGC131350.16942067159205515No Hit
TTCACAGTGGCTAAGTTCTG129000.1663895442358212No Hit
TGTAAACATCCTACACTCTCAGCT128010.16511260122191837No Hit
TAGTGGGTTATCAGAACT125810.16227495007991213No Hit
CTCGTACGACTCTTAGCGG124910.16111409279454592No Hit
TGAGGTAGTAGGTTGTATAGT119610.15427793322516722No Hit
TTCACAGTGGCTAAGTTCCG111210.14344326522841608No Hit
CAAAGAATTCTCCTTTTGGGCT109650.14143111260044802No Hit
TGAGGTAGTAGTTTGTGCTGTTA107230.13830969634424112No Hit
TTCAAGTAATCCAGGATAGGCTT106490.1373552136873845No Hit
CTCCCACTGCTTCACTTGACTAGCCT105200.13569131824502628No Hit
TGTCAGTTTGTCAAATACCCCAA104110.134285391088305No Hit
CCCATAAAGTAGAAAGCACTAC103900.1340145243883862No Hit
CCCCCCACTGCTAAATTTGACTGG98590.12716546640472567No Hit
TCGTTTCCCGGCCAACGCACCA97730.12605620277648685No Hit
TGAGGTAGTAGTTTGTACAGT96110.12396665966282772No Hit
TCCCACTGCTTCACTTGACTAG94650.12208349117767811No Hit
TATTGCACTTGTCCCGGCCTGTT93350.12040669732103805No Hit
TCGACCGGACCTCGACCGGCT90180.11631789999369269No Hit
CACTAGATTGTGAGCTCCTGGA89860.11590515073667358No Hit
AGCTCGGTCTGAGGCCCCTCAGT85770.11062969929539833No Hit
AATGCACCCGGGCAAGGATTCT84950.10957202932428692No Hit
TAGCAGCACATCATGGTTTACA84740.10930116262436813No Hit
TCAGTGCACTACAGAACTTTGTC84070.10843696886748441No Hit
CCCATAAAGTAGAAAGCACT83760.10803711802474716No Hit
ACCGGGTGCTGTAGGCTT83080.10716002585358159No Hit
TTCAAGTAATTCAGGATAGGT82150.10596047332536984No Hit
TCGGACGACTCTTAGCGG81820.10553482565406891No Hit
CAAAGTGCTGTTCGTGCAGGTAG80020.10321311108333651No Hit
TTCAAGTAATTCAGGATAGGTTA79740.10285195548344482No Hit
CAAAGAATTCTCCTTTTGGGCTT79190.10214254269794326No Hit
TAGCAGCACGTAAATATTGGCGT78110.10074951395550383No Hit
ATCACATTGCCAGGGATTTCC77540.10001430434143856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)