FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8200697
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG112103413.669984392790028No Hit
CCCCCCACTGCTAAATTTGACTG7419959.047950436408026No Hit
CCCCCACTGCTAAATTTGACTG2627813.2043739696759923No Hit
TTCAAGTAATCCAGGATAGGCT2268242.7659112390080987No Hit
AACATTCAACGCTGTCGGTGAGT1710172.0853959120791807No Hit
GCCTGTCTGAGCGTCGCT941941.1486096852499244No Hit
CGCCTGTCTGAGCGTCGCT926921.1302941688980828No Hit
TAGCAGCACGTAAATATTGGCG802730.978855821645404No Hit
AACATTCAACGCTGTCGGTGAG775580.9457488796379137No Hit
AAGCTGCCAGTTGAAGAACTGT726690.8861319958535232No Hit
CAACGGAATCCCAAAAGCAGCTGT721050.879254531657492No Hit
ACTGGACTTGGAGTCAGAAGGC697890.8510130297461301No Hit
CCCATAAAGTAGAAAGCACTAC620330.7564357029652479No Hit
AACCGGGCGGAAACACCA490460.5980711151747223No Hit
AGCAGCATTGTACAGGGCTATGA490120.5976565162692878No Hit
CATAAAGTAGAAAGCACTACT454710.5544772596768299No Hit
CTACGCCTGTCTGAGCGTCGCT424630.5177974506313304No Hit
CCCATAAAGTAGAAAGCACT417450.5090420972753901No Hit
TACGCCTGTCTGAGCGTCGCT387200.4721549887771735No Hit
TCCTGTCTGAGCGTCGCT374780.45700993464336015No Hit
CCCCCACTGCTAAATTTGACTGGCTT371660.45320537998172594No Hit
CATTGCACTTGTCTCGGTCTGA352870.43029269341374277No Hit
TCGTACGACTCTTAGCGG349830.42658569143574016No Hit
TAGCTTATCAGACTGATGTTGA341090.4159280607489827No Hit
TAGCTTATCAGACTGATGTTGAC337250.4112455319346636No Hit
CAACGGAATCCCAAAAGCAGCT331340.40403882743137565No Hit
CAACACCAGTCGATGGGCTGT308800.376553358818159No Hit
CCCCCCACTGCTAAATTTGACTGGCT304170.3709074972529774No Hit
TGTAAACATCCTACACTCTCAGCT293830.358298812893587No Hit
CACCCGGCTGTGTGCACATGTGC293400.35777446721906686No Hit
CCCCCCACTGCTAAATTTGACTGG283700.3459462043287296No Hit
CTCCCACTGCTTCACTTGACTAG282170.34408050925427436No Hit
ACGCCTGTCTGAGCGTCGCT256260.3124856338430746No Hit
CACTAGATTGTGAGCTCCTGGA221580.2701965454887554No Hit
TTCAAGTAATTCAGGATAGGTT220570.26896494285790584No Hit
AACGGAATCCCAAAAGCAGCTG215090.262282584029138No Hit
CACTAGATTGTGAGCTCCTGGAA200070.2439670676772962No Hit
CTTCTCACTACTGCACTTGACTAG196470.23957719691387208No Hit
ATCCCGGACGAGCCCCCA192190.23435812833957895No Hit
CAAAGAATTCTCCTTTTGGGCT190730.23257779186330135No Hit
ACTGGACTTGGAGTCAGAAGGCT187110.22816353292896935No Hit
ACTGGACTTGGAGTCAGAAGGCA180580.22020079512753613No Hit
CAAAGAATTCTCCTTTTGGGCTT166290.20277544701383307No Hit
CGCGACCTCAGATCAGACGT163950.1999220310176074No Hit
AGCTCGGTCTGAGGCCCCTCAGT163730.19965376113762037No Hit
GCGTTCGCGCTTTCCCCTG162500.1981538886267838No Hit
AACATTCAACGCTGTCGGTGAGTT158090.19277629694158924No Hit
TTCTCACTACTGCACTTGACTAG150210.18316735760387196No Hit
TTCAAGTAATCCAGGATAGGCTT144810.17658255145873578No Hit
ACTGGACTTGGAGTCAGAAGG142730.17404618168431293No Hit
TATTGCACTTGTCCCGGCCTGTAA141800.17291213173709505No Hit
ACCGGGTGCTGTAGGCTT141700.17279019088255548No Hit
TAGCTTATCAGACTGATGTTGACT141300.17230242746439722No Hit
ACCACAGGGTAGAACCACGGAC141240.1722292629516735No Hit
ATCCCACCGCTGCCACCA134470.16397386709934536No Hit
TATTGCACTTGTCCCGGCCTGT130830.15953521999410536No Hit
CCCCCCACTGCTAAATTTGACTGTA129010.15731589644148541No Hit
TGTAAACATCCTACACTCAGCT124720.1520846337817383No Hit
CGCGACCTCAGATCAGACGTGGC122370.14921902370005868No Hit
TGAGGTAGTAGGTTGTATAGTT122080.14886539522189396No Hit
ACCGGGTGCTGTAGGCTTT119820.1461095319092999No Hit
CCCATAAAGTAGAAAGCACTA118050.1439511787839497No Hit
CCTCGACACAAGGGTTTGT116360.14189037834223117No Hit
GGAATACCGGGTGCTGTAGGCTTT114550.1396832488750651No Hit
CTCGACACAAGGGTTTGT111690.13619574043523375No Hit
CCCCCACTGCTAAATTTGACTGGCT108550.13236679760269157No Hit
CCAAGTCCTTCTGATCGAGGCCC106860.13030599716097302No Hit
ATAGGCGATAGAAATTGAAACCT105760.1289646477610379No Hit
ACCGGGTGCTGTAGGCTTA103430.12612342585026615No Hit
TACCTGGTTGATCCTGCC100090.12205060130864487No Hit
TCGTTTCCCGGGCGGCGCACCA99140.12089216319051904No Hit
GACCCGCGGGCGCTCTCCAGTCCT98460.12006296537965004No Hit
TCAGTGCACTACAGAACTTTGT98420.12001418903783423No Hit
CCCCCACTGCTAAATTTGACTGGCTA96990.11827043481791853No Hit
TCGTTTCCCGGCCAATGCACCA96620.11781925365612217No Hit
AGCAGCATTGTACAGGGCTATGT96160.1172583257252402No Hit
CAGGTCCCTGTTCGGGCGCCA96120.11720954938342436No Hit
CAGTGCAATAGTATTGTCAAAGC95520.116477904256187No Hit
TATTGCACTTGTCCCGGCCTGTAT95050.11590478223985107No Hit
ATCACATTGCCAGGGATTTCC93590.11412444576357351No Hit
TTCACAGTGGCTAAGTTCCG91130.11112470074190034No Hit
CCCCCCACTGCTAAATTTGACTGT86600.10560078003125832No Hit
TTAATGCTAATCGTGATAGGGGTT84290.1027839462913945No Hit
ACGGGTCCCCGGTAGCTC83810.10219863018960462No Hit
CCCCCACTGCTAAATTTGACTGG83160.10140601463509748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)