FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267859
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6935656.754718778276951No Hit
CCCCCCACTGCTAAATTTGACTG6426836.259172433123594No Hit
AACATTCAACGCTGTCGGTGAGT4099853.992896669110863No Hit
AAGCTGCCAGTTGAAGAACTGT3195763.1123917848891383No Hit
TTCAAGTAATCCAGGATAGGCT3089953.0093420643972615No Hit
TAGCAGCACGTAAATATTGGCG2969482.89201478127037No Hit
AACATTCAACGCTGTCGGTGAG2262842.2038089927023736No Hit
CCCCCACTGCTAAATTTGACTG2261502.2025039494601555No Hit
TAGCTTATCAGACTGATGTTGAC2116922.0616956271020084No Hit
CGCCTGTCTGAGCGTCGCT2024331.9715210347161956No Hit
TAGCTTATCAGACTGATGTTGACT1384871.3487427125752312No Hit
TAGCTTATCAGACTGATGTTGA1231051.1989354353229822No Hit
TGAGGTAGTAGGTTGTATAGTT1183021.1521584003052632No Hit
TATTGCACTTGTCCCGGCCTGTAA1145631.1157437981959042No Hit
TATTGCACTTGTCCCGGCCTGT1021650.994998080904695No Hit
TATTGCACTTGTCCCGGCCTGTAT1017290.990751820803149No Hit
TGTAAACATCCTTGACTGGAAGCT981630.9560220879542659No Hit
AGCAGCATTGTACAGGGCTATGA882060.8590495837545101No Hit
ACTGGACTTGGAGTCAGAAGGC834780.8130029833872865No Hit
TACGCCTGTCTGAGCGTCGCT755190.7354892582767255No Hit
TGAGGTAGTAGATTGTATAGTT697580.6793821379900133No Hit
TATTGCACTTGTCCCGGCCTGTA672940.6553849249390744No Hit
GCCTGTCTGAGCGTCGCT614690.598654500417273No Hit
CTACGCCTGTCTGAGCGTCGCT610080.5941647620988951No Hit
CATAAAGTAGAAAGCACTACT579710.5645870283181723No Hit
TTCAAGTAATTCAGGATAGGTT536800.522796427181168No Hit
TTAATGCTAATCGTGATAGGGGTT523470.5098141686596982No Hit
TGTAAACATCCCCGACTGGAAGCT515500.5020520831071015No Hit
GCGTTCGCGCTTTCCCCTG479910.4673905241589313No Hit
ACGCCTGTCTGAGCGTCGCT435210.4238566189894115No Hit
AACATTCAACGCTGTCGGTGAGTT368470.3588576742240033No Hit
CAACGGAATCCCAAAAGCAGCTGT359870.35048202356499053No Hit
CATTGCACTTGTCTCGGTCTGA352780.34357698133564163No Hit
CCCATAAAGTAGAAAGCACTAC342260.33333141797136095No Hit
TGTCAGTTTGTCAAATACCCCAA334710.3259783758230416No Hit
CAACACCAGTCGATGGGCTGT323420.314982899550919No Hit
TGAGGTAGTAGTTTGTACAGTT313160.3049905535321434No Hit
TTCTCACTACTGCACTTGACTAG292530.28489873107918606No Hit
TGTAAACATCCTACACTCTCAGCT286620.2791429060332831No Hit
TCCTGTCTGAGCGTCGCT280950.2736208200755386No Hit
CCCATAAAGTAGAAAGCACT273070.26594638668100135No Hit
ACCGGGTGCTGTAGGCTT262560.2557105624453939No Hit
TAGCTTATCAGACTGATGTTGACA250970.24442291231307325No Hit
TCGTACGACTCTTAGCGG249960.24343926031707291No Hit
ACTGGACTTGGAGTCAGAAGGCA241860.2355505660917237No Hit
TCGTTCGCGCTTTCCCCTG237180.23099265387263304No Hit
ATCCCGGACGAGCCCCCA227690.22175022076169917No Hit
CAACGGAATCCCAAAAGCAGCT227430.22149700341619416No Hit
CTTCTCACTACTGCACTTGACTAG225030.21915961253460922No Hit
ACCGGGTGCTGTAGGCTTT224570.21871161261563876No Hit
TGAGGTAGTAGTTTGTGCTGTT222910.21709491725587582No Hit
CCTCGACACAAGGGTTTGT221320.2155463957968258No Hit
CACCCGGCTGTGTGCACATGTGC212200.20666431044680297No Hit
AACGGAATCCCAAAAGCAGCTG211250.20573909322284226No Hit
ACTGGACTTGGAGTCAGAAGG198680.19349700848054108No Hit
TTATAAAGCAATGAGACTGATT196790.19165631316129292No Hit
CTCCCACTGCTTCACTTGACTAG192690.18766327040525196No Hit
TTCAAGTAATCCAGGATAGGCTT190970.1859881402734494No Hit
TTCACAGTGGCTAAGTTCCG188510.18359231461982484No Hit
ACCGGGTGCTGTAGGCTTA187070.18218988009087386No Hit
TAGCAGCACGTAAATATTGGC186280.1814204889256855No Hit
TGTCAGTTTGTCAAATACCCCA183930.17913179368746687No Hit
CCAAGTCCTTCTGATCGAGGCCC182690.17792414173198132No Hit
ACTGGACTTGGAGTCAGAAGGCT176150.17155475157966232No Hit
CTGCGTTCGCGCTTTCCCCTG171990.16750327405158175No Hit
TGAGGTAGTAGGTTGTGTGGTT170910.1664514481548685No Hit
AGCAGCATTGTACAGGGCTATGT169480.1650587527545908No Hit
ACCACAGGGTAGAACCACGGAC167790.16341284000880807No Hit
CCCCCCACTGCTAAATTTGACTGG167180.16281875315973857No Hit
CAAGTCCTTCTGATCGAGGCCC166340.16200066635118382No Hit
ATCCCACCGCTGCCACCA166180.16184484029241147No Hit
CTCGACACAAGGGTTTGT163690.15941979725276711No Hit
TATTGCACTTGTCCCGGCCTGTT162970.1587185799882916No Hit
AGCAGCATTGTACAGGGCTATG159210.15505666760714187No Hit
CCCCCACTGCTAAATTTGACTGGCTT158450.15431649382797327No Hit
AACCGGGCGGAAACACCA157120.1530211897144283No Hit
TGTAAACATCCTACACTCAGCT151130.14718745163913918No Hit
TGTAAACATCCCCGACTGGAAGC146880.14304832195299916No Hit
TAGCAGCACGTAAATATTGGCGT146700.1428730176368803No Hit
TTCAAGTAATCCAGGATAGGC145700.14189910476955322No Hit
TACCTGGTTGATCCTGCC141880.13817875761636386No Hit
CCCCCCACTGCTAAATTTGACTGGCT138530.13491614951081815No Hit
CACTAGATTGTGAGCTCCTGGA136180.1326274542725996No Hit
TATTGCACTTGTCCCGGCCTGTAG136060.13251058472852031No Hit
TCGTTTCCCGGCCAATGCACCA128270.1249238034920425No Hit
CAAAGAATTCTCCTTTTGGGCT127870.12453423834511168No Hit
AGCTCGGTCTGAGGCCCCTCAGT127790.12445632531572551No Hit
CAAAGAATTCTCCTTTTGGGCTT124860.12160276061445723No Hit
GGAATACCGGGTGCTGTAGGCTTT124680.12142745629833834No Hit
CAAAGTGCTGTTCGTGCAGGT121750.11857389159707003No Hit
CAAAGTGCTGTTCGTGCAGGTAG117220.11416206630807844No Hit
TGAGGTAGTAGGTTGTATAGT116170.11313945779738502No Hit
AACATTCAACGCTGTCGGTGA114860.11186363194118658No Hit
GGGCTACGCCTGTCTGAGCGTCGCT114720.11172728413976077No Hit
CACTAGATTGTGAGCTCCTGGAA114420.11143511027956265No Hit
AATGACACGATCACTCCCGTTG112460.10952624105960163No Hit
TCGTTTCCCGGGCGGCGCACCA109660.10679928503108585No Hit
AACATTCAACGCTGTCGGTGAGA109300.10644867639884809No Hit
TTCACAGTGGCTAAGTTCTGC105430.10267963360229236No Hit
TTCACAGTGGCTAAGTTCTG103430.10073180786763823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)