FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9960252
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG124230012.472575995065185No Hit
CCCCCCACTGCTAAATTTGACTG8452408.486130672195843No Hit
TTCAAGTAATCCAGGATAGGCT3136833.149348028543856No Hit
AACATTCAACGCTGTCGGTGAGT2768712.779758986017623No Hit
CCCCCACTGCTAAATTTGACTG2592902.6032473877166966No Hit
TAGCAGCACGTAAATATTGGCG2349112.3584845042073233No Hit
AACATTCAACGCTGTCGGTGAG1838751.8460878299063115No Hit
AAGCTGCCAGTTGAAGAACTGT1650681.6572673060882397No Hit
TAGCTTATCAGACTGATGTTGAC1296841.3020152502165607No Hit
TATTGCACTTGTCCCGGCCTGTAA1179191.1838957488224193No Hit
TAGCTTATCAGACTGATGTTGACT1095851.1002231670443678No Hit
TAGCTTATCAGACTGATGTTGA1033751.0378753469289732No Hit
TATTGCACTTGTCCCGGCCTGTAT996051.0000248989684197No Hit
GCCTGTCTGAGCGTCGCT746500.7494790292454448No Hit
AGCAGCATTGTACAGGGCTATGA716430.7192890300365894No Hit
CATAAAGTAGAAAGCACTACT708310.7111366258604702No Hit
ACTGGACTTGGAGTCAGAAGGC705080.70789373602194No Hit
TCGTACGACTCTTAGCGG703030.7058355551646686No Hit
CAACGGAATCCCAAAAGCAGCTGT684110.6868400518380459No Hit
TGAGGTAGTAGGTTGTATAGTT635780.6383171831395431No Hit
CCCATAAAGTAGAAAGCACTAC633950.63647988022793No Hit
TATTGCACTTGTCCCGGCCTGT617030.6194923582254745No Hit
CAACGGAATCCCAAAAGCAGCT512310.5143544560920749No Hit
TTCAAGTAATTCAGGATAGGTT501270.5032703991826714No Hit
CGCCTGTCTGAGCGTCGCT457270.4590948100509907No Hit
TATTGCACTTGTCCCGGCCTGTA455930.45774946256379856No Hit
CCCCCACTGCTAAATTTGACTGGCTT455240.45705670900696094No Hit
TGAGGTAGTAGATTGTATAGTT451060.4528600280394512No Hit
TGTAAACATCCTTGACTGGAAGCT425330.4270273483040389No Hit
CACCCGGCTGTGTGCACATGTGC416510.4181721506644611No Hit
CCCATAAAGTAGAAAGCACT412960.41460798381406416No Hit
ATCCCGGACGAGCCCCCA406810.4084334412422497No Hit
CTCCCACTGCTTCACTTGACTAG397550.39913648771135507No Hit
CATTGCACTTGTCTCGGTCTGA384900.3864360058359969No Hit
TGTAAACATCCCCGACTGGAAGCT328300.3296101343620623No Hit
CCCCCCACTGCTAAATTTGACTGGCT322680.32396770684115217No Hit
CTTCTCACTACTGCACTTGACTAG316930.31819476053417123No Hit
CACTAGATTGTGAGCTCCTGGAA308920.3101527953308812No Hit
CTACGCCTGTCTGAGCGTCGCT306550.30777333746174296No Hit
TGTAAACATCCTACACTCTCAGCT303790.3050023232343921No Hit
TTCTCACTACTGCACTTGACTAG294080.2952535739055598No Hit
CAACACCAGTCGATGGGCTGT293540.29471141894803465No Hit
TCGTTTCCCGGCCAATGCACCA284150.28528394663106915No Hit
CACTAGATTGTGAGCTCCTGGA282160.2832860052135227No Hit
AGCTCGGTCTGAGGCCCCTCAGT276420.27752309881316256No Hit
CAAAGAATTCTCCTTTTGGGCT265270.26632860293092986No Hit
AACGGAATCCCAAAAGCAGCTG244850.24582711361118173No Hit
AACATTCAACGCTGTCGGTGAGTT241750.2427147425587224No Hit
CCCCCCACTGCTAAATTTGACTGG241010.24197178946878053No Hit
TCGTTTCCCGGGCGGCGCACCA238280.23923089496129213No Hit
AACCGGGCGGAAACACCA221840.22272528847663695No Hit
ACTGGACTTGGAGTCAGAAGGCA207380.20820758350290738No Hit
TAGCTTATCAGACTGATGTTGACA198040.19883031071904608No Hit
TACGCCTGTCTGAGCGTCGCT197820.1986094327733877No Hit
ACTGGACTTGGAGTCAGAAGGCT192340.19310756394516926No Hit
GGAATACCGGGTGCTGTAGGCTTT187670.1884189275532386No Hit
ATCCCACCGCTGCCACCA186500.18724425847860074No Hit
TTCAAGTAATCCAGGATAGGCTT179860.18057776048236532No Hit
CAAAGAATTCTCCTTTTGGGCTT176540.17724451148424758No Hit
TCCTGTCTGAGCGTCGCT176000.17670235652672242No Hit
AGCAGCATTGTACAGGGCTATGT159240.15987547303020044No Hit
TGTCAGTTTGTCAAATACCCCAA152490.1530985360611358No Hit
CAACGGAATCCCAAAAGCAGCTGA152300.1529077778353399No Hit
TAGCAGCACGTAAATATTGGC150550.15115079417669353No Hit
CCCATAAAGTAGAAAGCACTA146990.14757658741967572No Hit
TGAGGTAGTAGTTTGTACAGTT145110.14568908497495847No Hit
ACTGGACTTGGAGTCAGAAGG144970.14554852628226675No Hit
CCCCCCACTGCTAAATTTGACTGA144340.14491601216515404No Hit
ACGCCTGTCTGAGCGTCGCT143560.1441328994487288No Hit
TTCAAGTAATCCAGGATAGGC140790.1413518453147571No Hit
TTCACAGTGGCTAAGTTCCG136200.13674352817579313No Hit
CCCCCCACTGCTAAATTTGACTGTA134250.13478574638473004No Hit
ACCACAGGGTAGAACCACGGAC131020.13154285654619985No Hit
CTCCCACTGCTTCACTTGACTAGCCT126280.12678394080792332No Hit
ACCCCCCACTGCTAAATTTGACTG125890.1263923844497107No Hit
TATTGCACTTGTCCCGGCCTGTAG124860.12535827406776456No Hit
ATACCGGGTGCTGTAGGCTTA123390.12388240779450158No Hit
GGAATACCGGGTGCTGTAGGCTT122740.12322981386414722No Hit
ACCGGGTGCTGTAGGCTTA122540.12302901573173047No Hit
TGTAAACATCCCCGACTGGAAGC120790.1212720320730841No Hit
TGAGGTAGTAGTTTGTGCTGTT116210.11667375484074097No Hit
GACCCGCGGGCGCTCTCCAGTCCT115470.11593080175079906No Hit
TATTGCACTTGTCCCGGCCTGTT114320.11477621248940288No Hit
TGTCAGTTTGTCAAATACCCCA112320.11276823116523557No Hit
CCCCCCACTGCTAAATTTGACTGT112150.11259755275268137No Hit
ATCACATTGCCAGGGATTTCC112130.11257747293943968No Hit
TTCACAGTGGCTAAGTTCTGC110710.1111518061992809No Hit
TCAGTGCACTACAGAACTTTGT110660.11110160666617673No Hit
CCCCCACTGCTAAATTTGACTGGCTA109060.10949522160684287No Hit
TTCACAGTGGCTAAGTTCCGC107900.10833059243882585No Hit
AGCAGCATTGTACAGGGCTATG107350.10777839757467984No Hit
CCCCCACTGCTAAATTTGACTGGCT105000.10541901951878327No Hit
TATTGCACTTGTCCCGGCCTGTAAT99900.10029866714215666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)