FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19196157
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG18380729.575208204433835No Hit
CAACGGAATCCCAAAAGCAGCTG15622018.138092431729955No Hit
AACATTCAACGCTGTCGGTGAGT8900404.636553035068426No Hit
CCCCCACTGCTAAATTTGACTG6318643.291617171082733No Hit
TAGCAGCACGTAAATATTGGCG5460342.8444964270713142No Hit
TTCAAGTAATCCAGGATAGGCT5438702.833223337358618No Hit
AACATTCAACGCTGTCGGTGAG4898292.5517034477265423No Hit
AAGCTGCCAGTTGAAGAACTGT4673922.434820677909646No Hit
TATTGCACTTGTCCCGGCCTGTAA3302371.7203287095432693No Hit
TATTGCACTTGTCCCGGCCTGTAT2757271.4363656225566397No Hit
TAGCTTATCAGACTGATGTTGACT2668661.390205341621242No Hit
TGAGGTAGTAGGTTGTATAGTT2484461.29424863528674No Hit
TAGCTTATCAGACTGATGTTGAC2213221.152949520052373No Hit
GGAATACCGGGTGCTGTAGGCTTT1904880.9923236197745204No Hit
TATTGCACTTGTCCCGGCCTGT1764140.9190068616338155No Hit
TAGCTTATCAGACTGATGTTGA1605150.836182992252043No Hit
GGAATACCGGGTGCTGTAGGCTT1504840.783927741370317No Hit
TGAGGTAGTAGATTGTATAGTT1355840.7063080386350247No Hit
TTCAAGTAATTCAGGATAGGTT1306910.6808185617569182No Hit
TGTAAACATCCTTGACTGGAAGCT1166380.607611200512686No Hit
TATTGCACTTGTCCCGGCCTGTA1096690.5713070590118637No Hit
TTCTCACTACTGCACTTGACTAG1008630.5254332937577038No Hit
CTCCCACTGCTTCACTTGACTAG979740.510383406428693No Hit
AGCAGCATTGTACAGGGCTATGA967340.5039237801607895No Hit
CAACGGAATCCCAAAAGCAGCTGT940880.490139771205247No Hit
TGTAAACATCCCCGACTGGAAGCT889900.46358237224252746No Hit
ACTGGACTTGGAGTCAGAAGGC853610.44467754665686476No Hit
CATAAAGTAGAAAGCACTACT784000.40841508016422245No Hit
CTTCTCACTACTGCACTTGACTAG773890.4031484010054721No Hit
GGGAATACCGGGTGCTGTAGGCTTT763370.3976681374297991No Hit
GGGAATACCGGGTGCTGTAGGCTT725800.378096511713256No Hit
CATTGCACTTGTCTCGGTCTGA715240.37259541063349294No Hit
TCGTACGACTCTTAGCGG611210.3184022718713959No Hit
AGCTCGGTCTGAGGCCCCTCAGT607130.3162768464542148No Hit
TGAGAACTGAATTCCATGGGTT563480.2935379201159899No Hit
GAATACCGGGTGCTGTAGGCTTT556900.290110150693183No Hit
AACATTCAACGCTGTCGGTGAGTT534920.27865994219572177No Hit
CCCATAAAGTAGAAAGCACT528810.2754770134459726No Hit
TGAGGTAGTAGTTTGTACAGTT492220.25641590657963464No Hit
CCCATAAAGTAGAAAGCACTAC486660.2535194935111231No Hit
CAACACCAGTCGATGGGCTGT471070.24539807629204113No Hit
CCCCCCACTGCTAAATTTGACTGG459620.23943334074627542No Hit
CACTAGATTGTGAGCTCCTGGA441240.22985850761691518No Hit
CTCCCACTGCTTCACTTGACTAGCCT431460.22476373786690743No Hit
ACCGGGTGCTGTAGGCTTA425200.22150266847682062No Hit
GAATACCGGGTGCTGTAGGCTT411210.2142147514213392No Hit
TGATGTAGTAGGTTGTATAGTT402090.2094638004888166No Hit
CGCCTGTCTGAGCGTCGCT394870.20570263100056957No Hit
TATTGCACTTGTCCCGGCCTGTAG394160.20533276530297187No Hit
CGCGACCTCAGATCAGACGTGGCG381490.19873248588246073No Hit
ACCGGGTGCTGTAGGCTT377570.19669041048163963No Hit
CCCCCCACTGCTAAATTTGACTGT375060.19538285709999142No Hit
AACATTCAACGCTGTCGGTGA368210.19181443452457697No Hit
TGAGGTAGTAGTTTGTGCTGTT364470.18986612789216092No Hit
AACGGAATCCCAAAAGCAGCTG364460.18986091851613843No Hit
AACCGGGCGGAAACACCA361350.18824080257314002No Hit
CCCCCACTGCTAAATTTGACTGGCTT353250.18402120799491273No Hit
CCCCCCACTGCTAAATTTGACTGGCT350580.1826303045969045No Hit
TGTAAACATCCTACACTCTCAGCT345230.17984328842486547No Hit
CACTAGATTGTGAGCTCCTGGAA342430.17838466313856466No Hit
CGCGACCTCAGATCAGACGTGG338230.17619672520911348No Hit
CTACGCCTGTCTGAGCGTCGCT319070.16621556074999802No Hit
ACCGGGTGCTGTAGGCTTT309270.16111037224794525No Hit
ACCCCCCACTGCTAAATTTGACTG304110.1584223342203338No Hit
GGAATACCGGGTGCTGTAGGCTTA301700.1571668745989106No Hit
CCCCCCACTGCTAAATTTGACTGTA299570.1560572775061175No Hit
TTCAAGTAATCCAGGATAGGCTT299480.15601039312191498No Hit
TATTGCACTTGTCCCGGCCTGTT293080.15267639246751316No Hit
GCCTGTCTGAGCGTCGCT292180.15220754862548788No Hit
CACCCGGCTGTGTGCACATGTGC289310.1507124577070296No Hit
TGAGAACTGAATTCCATAGGCTGT280410.14607611304700208No Hit
TAGCAGCACGTAAATATTGGC279180.14543535979623423No Hit
CAAAGAATTCTCCTTTTGGGCT278790.1452321941313566No Hit
TGAGGTAGTAGGTTGTGTGGTT271630.14150228089924458No Hit
TATTGCACTTGTCCCGGCCTGTAAT262290.1366367236942269No Hit
GGAATACCGGGTGCTGTAGGCT255970.13334439804800513No Hit
TGTAAACATCCCCGACTGGAAGC252860.13172428210500675No Hit
TTCAAGTAATCCAGGATAGGC252070.13131274139922902No Hit
CAAAGAATTCTCCTTTTGGGCTT251240.13088036318936128No Hit
CCCGCCACTGCTAAATTTGACTG246500.12841111895469495No Hit
TAGCTTATCAGACTGATGTTGACA245900.12809855639334478No Hit
ATACCGGGTGCTGTAGGCTTA243880.1270462624367992No Hit
CAACGGAATCCCAAAAGCAGCT243620.12691081866021411No Hit
TGGGAATACCGGGTGCTGTAGGCTTT237880.1239206368232975No Hit
AACATTCATTGCTGTCGGTGGGT229440.1195239234603051No Hit
ACTGGACTTGGAGTCAGAAGGCT227880.11871126080079467No Hit
AATGACACGATCACTCCCGTTG226050.11775794498867663No Hit
CGCGACCTCAGATCAGACGTGGC225540.117492266811529No Hit
TGGGAATACCGGGTGCTGTAGGCTT223780.1165754166315685No Hit
AACATTCAACGCTGTCGGTGAGA222750.11603885090125071No Hit
ATCCCGGACGAGCCCCCA222690.11600759464511567No Hit
TGATGTAGTAGATTGTATAGTT217740.11342895351397679No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC214460.11172027817859585No Hit
CAAGGGAATCCCAAAAGCAGCTG213140.11103264054362547No Hit
TTCACAGTGGCTAAGTTCTG205660.10713602727879334No Hit
AACATTCATTGCTGTCGGTGGG204300.10642755213973298No Hit
CAAAGTGCTGTTCGTGCAGGT194950.10155678555869281No Hit
TATTGCACTTGTCCCGGCCTGTTT194760.10145780741426527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)