FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13690696
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG289474621.143892173195578No Hit
CAACGGAATCCCAAAAGCAGCTG246410917.998420241016237No Hit
AACATTCAACGCTGTCGGTGAGT8722396.371034752360289No Hit
CCCCCACTGCTAAATTTGACTG7177255.242428872863732No Hit
AACATTCAACGCTGTCGGTGAG4669763.410900366204903No Hit
TAGCAGCACGTAAATATTGGCG4446443.2477822895198316No Hit
AAGCTGCCAGTTGAAGAACTGT3202062.338858448102273No Hit
TTCAAGTAATCCAGGATAGGCT2967832.167771455885077No Hit
TATTGCACTTGTCCCGGCCTGTAA2816162.056988191104382No Hit
TATTGCACTTGTCCCGGCCTGTAT2336211.7064216457658543No Hit
TATTGCACTTGTCCCGGCCTGT1229280.8978944532841865No Hit
TATTGCACTTGTCCCGGCCTGTA964850.7047486848002468No Hit
TAGCTTATCAGACTGATGTTGAC837280.6115686156496354No Hit
CAACGGAATCCCAAAAGCAGCTGT824030.6018905101683655No Hit
TGAGGTAGTAGGTTGTATAGTT787000.574842944434673No Hit
TAGCTTATCAGACTGATGTTGACT776970.5675168011911155No Hit
TGTAAACATCCTTGACTGGAAGCT588420.4297955341350067No Hit
AGCAGCATTGTACAGGGCTATGA486010.3549929090529802No Hit
TAGCTTATCAGACTGATGTTGA440450.3217148346585155No Hit
AACATTCAACGCTGTCGGTGAGTT436530.31885157628217004No Hit
AACGGAATCCCAAAAGCAGCTG382550.27942333976300404No Hit
ACTGGACTTGGAGTCAGAAGGC382210.27917499592423933No Hit
CCCCCCACTGCTAAATTTGACTGG374580.2736018680131383No Hit
CCCCCACTGCTAAATTTGACTGGCTT357500.26112624223048997No Hit
TGTAAACATCCCCGACTGGAAGCT354960.2592709676703069No Hit
TGAGGTAGTAGATTGTATAGTT346810.25331801977050694No Hit
TATTGCACTTGTCCCGGCCTGTAG345880.25263872632918005No Hit
ACCCCCCACTGCTAAATTTGACTG333930.24391017081965738No Hit
CGCCTGTCTGAGCGTCGCT314820.22995178623497298No Hit
CTCCCACTGCTTCACTTGACTAG305090.22284476990797253No Hit
AACATTCAACGCTGTCGGTGAGA300590.21955786615961673No Hit
CATAAAGTAGAAAGCACTACT297900.2175930281411551No Hit
TTCAAGTAATTCAGGATAGGTT281040.20527809543064868No Hit
TTCTCACTACTGCACTTGACTAG275970.20157485054083443No Hit
ACCGGGTGCTGTAGGCTT264590.19326263617277017No Hit
CCCATAAAGTAGAAAGCACTAC252650.1845413848937994No Hit
CATTGCACTTGTCTCGGTCTGA252020.18408121836902958No Hit
TATTGCACTTGTCCCGGCCTGTT235350.17190506603900926No Hit
CCCCCCACTGCTAAATTTGACTGGCT224980.16433057895668707No Hit
CTTCTCACTACTGCACTTGACTAG217450.15883049335110502No Hit
CCCCCCACTGCTAAATTTGACTGT201850.14743589369013818No Hit
TGTAAACATCCTACACTCTCAGCT191330.13975184314953748No Hit
CTACGCCTGTCTGAGCGTCGCT184850.13501870175190508No Hit
CTCCCACTGCTTCACTTGACTAGCCT180380.13175371069520497No Hit
TGAGGTAGTAGTTTGTACAGTT176720.1290803623132089No Hit
CAACGGAATCCCAAAAGCAGCT170170.12429609130171322No Hit
ACCGGGTGCTGTAGGCTTA160520.11724750881912796No Hit
TTAATGCTAATCGTGATAGGGGTT153530.11214185166334859No Hit
ACCGGGTGCTGTAGGCTTT150660.11004553749495277No Hit
TATTGCACTTGTCCCGGCCTGTTT146480.10699236912425782No Hit
TATTGCACTTGTCCCGGCCTGTAAT145760.1064664645245209No Hit
AACATTCAACGCTGTCGGTGA139840.102142360037795No Hit
CAACACCAGTCGATGGGCTGT138730.1013315904465339No Hit
TAGCAGCACGTAAATATTGGC138280.10100290007169833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)