FastQCFastQC Report
Wed 8 May 2024
EGAF00003947050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6759711
Total Bases154.2 Mbp
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6566479.71412831110679No Hit
CAACGGAATCCCAAAAGCAGCTG5135637.597410599358463No Hit
AACATTCAACGCTGTCGGTGAGT2484243.6750683572123126No Hit
GGAATACCGGGTGCTGTAGGCTTT2224543.2908803349729No Hit
CCCCCACTGCTAAATTTGACTG1785872.6419324731486302No Hit
TAGCAGCACGTAAATATTGGCG1506672.2288970637945913No Hit
GGAATACCGGGTGCTGTAGGCTT1456362.154470805038854No Hit
TTCAAGTAATCCAGGATAGGCT1430892.116791679407596No Hit
AACATTCAACGCTGTCGGTGAG1423442.105770498176623No Hit
TATTGCACTTGTCCCGGCCTGTAA1379702.0410635898487373No Hit
AAGCTGCCAGTTGAAGAACTGT1328641.9655278162039769No Hit
TATTGCACTTGTCCCGGCCTGTAT1284611.9003918954523351No Hit
TAGCTTATCAGACTGATGTTGACT792941.173038314803695No Hit
TAGCTTATCAGACTGATGTTGAC728381.0775312731565003No Hit
TATTGCACTTGTCCCGGCCTGT622390.9207346290396143No Hit
TGAGGTAGTAGGTTGTATAGTT549880.8134667295687641No Hit
TGTAAACATCCTTGACTGGAAGCT535860.7927261979099401No Hit
TATTGCACTTGTCCCGGCCTGTA509270.7533901967110724No Hit
TGAATACCGGGTGCTGTAGGCTTT503900.7454460701056599No Hit
GCCTGTCTGAGCGTCGCT392530.5806905058515076No Hit
GGAATACCGGGTGCTGTAGGCTTA392330.5803946352144345No Hit
AGCAGCATTGTACAGGGCTATGA382800.5662963993579015No Hit
TAGCTTATCAGACTGATGTTGA377950.5591215364088791No Hit
TGAGGTAGTAGATTGTATAGTT348710.5158652492687927No Hit
TTAATGCTAATCGTGATAGGGGTT332750.4922547724303598No Hit
TGAATACCGGGTGCTGTAGGCTT330760.48931085959148257No Hit
TGTAAACATCCCCGACTGGAAGCT327500.4844881682071911No Hit
ACTGGACTTGGAGTCAGAAGGC315730.4670761812154396No Hit
CTCCCACTGCTTCACTTGACTAG314260.46490153203295226No Hit
TTCAAGTAATTCAGGATAGGTT308900.45697219895939345No Hit
CAACGGAATCCCAAAAGCAGCTGT307540.4549602786272963No Hit
CATAAAGTAGAAAGCACTACT284600.4210239165550125No Hit
CCCATAAAGTAGAAAGCACTAC284210.42044696881272No Hit
GGAATACCGGGTGCTGTAGGCT222110.32857913600152433No Hit
TTCTCACTACTGCACTTGACTAG212930.3149986737598693No Hit
CCCCCACTGCTAAATTTGACTGGCTT202820.3000424130558244No Hit
AACATTCAACGCTGTCGGTGAGTT191750.2836659732938287No Hit
ACCCCCCACTGCTAAATTTGACTG190850.28233455542699976No Hit
TATTGCACTTGTCCCGGCCTGTAG188050.27819236650797646No Hit
AGCTCGGTCTGAGGCCCCTCAGT186280.2755739113698796No Hit
CATTGCACTTGTCTCGGTCTGA171950.2543747802235924No Hit
CCCCCCACTGCTAAATTTGACTGGCT163960.24255474827252232No Hit
CTTCTCACTACTGCACTTGACTAG159440.23586807187467038No Hit
TGTAAACATCCTACACTCTCAGCT157790.23342713911881735No Hit
CTCCCACTGCTTCACTTGACTAGCCT157460.23293895256764674No Hit
CACTAGATTGTGAGCTCCTGGAA155430.22993586560135487No Hit
AACCGGGCGGAAACACCA152050.22493565183481956No Hit
TCGTACGACTCTTAGCGG151760.22450663941106355No Hit
CGCGACCTCAGATCAGACGTGGCG150310.22236157729228365No Hit
TGAGGTAGTAGTTTGTACAGTT147420.21808624658657744No Hit
ACCGGGTGCTGTAGGCTTA137390.2032483341373618No Hit
TCGTTTCCCGGGCGGCGCACCA136790.20236072222614251No Hit
CACTAGATTGTGAGCTCCTGGA135920.20107368495487454No Hit
CCCCCCACTGCTAAATTTGACTGT134460.19891382930424095No Hit
CGCCTGTCTGAGCGTCGCT131490.1945201503437055No Hit
CCCCCCACTGCTAAATTTGACTGG128890.19067383206175528No Hit
TATTGCACTTGTCCCGGCCTGTT123230.18230069303258675No Hit
CCCATAAAGTAGAAAGCACT121790.18017042444566048No Hit
CAAAGAATTCTCCTTTTGGGCT120960.17894256130180713No Hit
TATTGCACTTGTCCCGGCCTGTAAT118870.1758507131443933No Hit
TAGCTTATCAGACTGATGTTGACA114170.1688977531731756No Hit
TATTGCACTTGTCCCGGCCTGTTT113730.1682468377716148No Hit
TGAGAACTGAATTCCATGGGTT111090.16434134536224995No Hit
TATTGCACTTGTCCCGGCCTGTAAA107840.1595334475098122No Hit
ACCGGGTGCTGTAGGCTT106480.15752152717771514No Hit
AACGGAATCCCAAAAGCAGCTG105390.15590903220566676No Hit
CACCCGGCTGTGTGCACATGTGC101100.1495626070404489No Hit
CGCGACCTCAGATCAGACGTGG97250.14386709727679187No Hit
CCCCCCACTGCTAAATTTGACTGTA94840.14030185610006107No Hit
TGAGGTAGTAGTTTGTGCTGTT93910.1389260576376712No Hit
TGAATACCGGGTGCTGTAGGCTTA91060.1347099010593796No Hit
TCCTGTCTGAGCGTCGCT88540.13098193103225864No Hit
CAAAGAATTCTCCTTTTGGGCTT88290.13061209273591726No Hit
TTCAAGTAATCCAGGATAGGCTT86830.12845223708528367No Hit
ACCGGGTGCTGTAGGCTTT84370.12481302824928463No Hit
GGGAATACCGGGTGCTGTAGGCTT82820.12252003081196815No Hit
CTACGCCTGTCTGAGCGTCGCT82690.12232771489787064No Hit
TAGCAGCACGTAAATATTGGC81170.12007909805611511No Hit
GGGAATACCGGGTGCTGTAGGCTTT79860.11814114538328635No Hit
TGTAAACATCCCCGACTGGAAGC78360.11592211560523816No Hit
ATCCCGGACGAGCCCCCA78310.11584814794596987No Hit
AGCAGCATTGTACAGGGCTATGT75630.11188348140919044No Hit
TGAGGGGCAGAGAGCGAGACTTT75530.11173554609065388No Hit
GAATACCGGGTGCTGTAGGCTTT74160.10970883222670318No Hit
CAACGGAATCCCAAAAGCAGCT73220.10831824023245964No Hit
ACTGGACTTGGAGTCAGAAGGCA72710.10756377010792326No Hit
ATGCACCTGGGCAAGGATTCTGA72520.10728269300270381No Hit
CAAAGTGCTGTTCGTGCAGGTAG72310.10697202883377707No Hit
TGAGGTAGTAGGTTGTGTGGTT72180.10677971291967955No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC71460.10571457862621642No Hit
ACTGGACTTGGAGTCAGAAGGCT70440.10420563837714365No Hit
CCCCCCACTGCTAAATTTGACTGA70180.10382100654894862No Hit
CAACACCAGTCGATGGGCTGT70130.10374703888968034No Hit
AGCTACATCTGGCTACTGGGTCTC68600.10148362851607118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)