FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889371
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5495527.976809493929126No Hit
CCCCCCACTGCTAAATTTGACTG5424107.87314255539439No Hit
TTCAAGTAATCCAGGATAGGCT1823962.647498588768118No Hit
AACATTCAACGCTGTCGGTGAGT1816352.6364525876164895No Hit
CCCCCACTGCTAAATTTGACTG1694142.459063389096044No Hit
AAGCTGCCAGTTGAAGAACTGT1487922.1597327245114246No Hit
AACATTCAACGCTGTCGGTGAG1233421.790323093356418No Hit
TATTGCACTTGTCCCGGCCTGTAA1129271.6391481892904303No Hit
TATTGCACTTGTCCCGGCCTGTAT1062681.5424920504353736No Hit
TAGCAGCACGTAAATATTGGCG891411.2938917065142812No Hit
TATTGCACTTGTCCCGGCCTGT752101.0916816644073892No Hit
TAGCTTATCAGACTGATGTTGACT600380.8714583668088132No Hit
TAGCTTATCAGACTGATGTTGAC591440.8584818556004604No Hit
TATTGCACTTGTCCCGGCCTGTA537130.7796502757653783No Hit
TGAGGTAGTAGGTTGTATAGTT477630.6932853521751114No Hit
TGTAAACATCCTTGACTGGAAGCT448480.6509737971724849No Hit
ACCGGGTGCTGTAGGCTT380880.552851631883375No Hit
CAACGGAATCCCAAAAGCAGCT374200.5431555362601317No Hit
TAGCTTATCAGACTGATGTTGA364030.5283936661271399No Hit
ACTGGACTTGGAGTCAGAAGGC359260.5214699571267101No Hit
GCCTGTCTGAGCGTCGCT356720.5177831183717643No Hit
AGCAGCATTGTACAGGGCTATGA334900.48611114135093025No Hit
CAACGGAATCCCAAAAGCAGCTGT332580.4827436350865703No Hit
ACCGGGTGCTGTAGGCTTA323670.469810669217843No Hit
TTAATGCTAATCGTGATAGGGGTT310800.4511297185185701No Hit
TACGCCTGTCTGAGCGTCGCT304230.4415932891406196No Hit
TGTAAACATCCCCGACTGGAAGCT303290.44022886849902554No Hit
CTCCCACTGCTTCACTTGACTAG286980.41655471885604656No Hit
CTACGCCTGTCTGAGCGTCGCT260350.3779009723819489No Hit
ACCGGGTGCTGTAGGCTTT259140.37614464368372674No Hit
CGTTCGCGCTTTCCCCTG229080.3325122133791314No Hit
TGAGGTAGTAGATTGTATAGTT224720.32618362402024803No Hit
TTCTCACTACTGCACTTGACTAG217020.31500698684974293No Hit
TTCAAGTAATTCAGGATAGGTT209580.30420774262265743No Hit
CATAAAGTAGAAAGCACTACT207780.30159502224513673No Hit
GGAATACCGGGTGCTGTAGGCTTT204960.29750176032035436No Hit
TCGTACGACTCTTAGCGG203770.29577446184854905No Hit
CATTGCACTTGTCTCGGTCTGA200770.29141992788601456No Hit
CCCCCACTGCTAAATTTGACTGGCTT195280.2834511307345765No Hit
GGAATACCGGGTGCTGTAGGCTT180620.2621719747709914No Hit
CGCCTGTCTGAGCGTCGCT179330.2602995251671016No Hit
CCCATAAAGTAGAAAGCACTAC171080.24832455677013185No Hit
AGCTCGGTCTGAGGCCCCTCAGT168640.24478286914727052No Hit
TCGTTTCCCGGCCAATGCACCA162230.23547868158065519No Hit
TATTGCACTTGTCCCGGCCTGTAG157780.22901945620289574No Hit
CTTCTCACTACTGCACTTGACTAG156880.22771309601413542No Hit
CAACGGAATCCCAAAAGCAGCTGA148810.2159993996549177No Hit
TCCTGTCTGAGCGTCGCT148170.215070432409577No Hit
ACCCCCCACTGCTAAATTTGACTG146560.21273349918301687No Hit
TGAGGTAGTAGTTTGTACAGTT141210.20496791361649708No Hit
TATTGCACTTGTCCCGGCCTGTT137240.1992054136727431No Hit
CTCCCACTGCTTCACTTGACTAGCCT135500.19667978397447314No Hit
CACCCGGCTGTGTGCACATGTGC131600.19101888982317833No Hit
AACGGAATCCCAAAAGCAGCTG129310.1876949288984437No Hit
AATGCACCCGGGCAAGGATTCT128720.18683853721914526No Hit
AACATTCAACGCTGTCGGTGAGTT124670.18095991636972375No Hit
TGAGGTAGTAGTTTGTGCTGTT124010.18000191889796616No Hit
CCCATAAAGTAGAAAGCACT122260.17746177408648772No Hit
CACTAGATTGTGAGCTCCTGGA122090.1772150171619441No Hit
TGAGAACTGAATTCCATGGGTT120950.175560294256181No Hit
CAAAGAATTCTCCTTTTGGGCT116950.16975424897280172No Hit
CGCGACCTCAGATCAGACGTGGCG114780.16660446940656848No Hit
CGCGACCTCAGATCAGACGTGG114770.16658995429336002No Hit
TGTAAACATCCTACACTCTCAGCT114490.16618353112352346No Hit
CCCCCCACTGCTAAATTTGACTGGCT112480.16326599336862538No Hit
CAACACCAGTCGATGGGCTGT111750.16220639010440865No Hit
CACTAGATTGTGAGCTCCTGGAA109610.15910015587780074No Hit
CCCCCCACTGCTAAATTTGACTGG108740.15783734102866576No Hit
ATCCCGGACGAGCCCCCA105320.15287317231137645No Hit
ACTGGACTTGGAGTCAGAAGGCT103670.1504781786319825No Hit
TGAGGTAGTAGGTTGTGTGGTT102740.14912827310359683No Hit
TATTGCACTTGTCCCGGCCTGTTT95700.1389096334048493No Hit
TCGTTTCCCGGGCGGCGCACCA92350.13404707048001915No Hit
GAATACCGGGTGCTGTAGGCTTT90310.1310859873854957No Hit
TTCACAGTGGCTAAGTTCTGC89640.13011347480052968No Hit
GGGAATACCGGGTGCTGTAGGCTT88080.12784911714001176No Hit
CCCCCCACTGCTAAATTTGACTGT86370.12536703278136713No Hit
TGAATACCGGGTGCTGTAGGCTTT85190.12365424942277023No Hit
ACTGGACTTGGAGTCAGAAGGCA82750.12011256179990887No Hit
TTCACAGTGGCTAAGTTCCG82280.1194303514791118No Hit
TAGCTTATCAGACTGATGTTGACA81500.11829817264885284No Hit
TATTGCACTTGTCCCGGCCTGA81210.11787723436580785No Hit
ATGCACCTGGGCAAGGATTCTGA78570.11404524447877754No Hit
ACTGGACTTGGAGTCAGAAGG78490.11392912357310993No Hit
TGTAAACATCCCCGACTGGAAGC77370.11230343089376374No Hit
AGCTACATCTGGCTACTGGGTCTC76470.1109970707050034No Hit
GGGAATACCGGGTGCTGTAGGCTTT75270.10925525711998961No Hit
CCCCCCACTGCTAAATTTGACTGTA74290.1078327760255617No Hit
TGAATACCGGGTGCTGTAGGCTT74020.10744086796893358No Hit
TGAGGGGCAGAGAGCGAGACTTT73970.10736829240289136No Hit
TATTGCACTTGTCCCGGCCTGTAAT73710.1069908994594717No Hit
TATTGCACTTGTCCCGGCCTGTAAA73080.10607644732733947No Hit
TTCAAGTAATCCAGGATAGGCTT72090.10463945111970309No Hit
TTCAAGTAATCCAGGATAGGC71850.10429108840270032No Hit
CGCGACCTCAGATCAGACGTGGC70780.10273797128939638No Hit
GTGCGGTAACGCGACCGATCCCGGA70420.10221542721389225No Hit
GAATACCGGGTGCTGTAGGCTT69700.10117033906288397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)