FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8422458
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5608706.659219909437364No Hit
AACATTCAACGCTGTCGGTGAGT5121156.080350890440771No Hit
CCCCCCACTGCTAAATTTGACTG3996314.744826272805397No Hit
TAGCAGCACGTAAATATTGGCG2611213.100294474605869No Hit
AACATTCAACGCTGTCGGTGAG2600103.0871035510061313No Hit
TTCAAGTAATCCAGGATAGGCT2432302.887874299877779No Hit
AAGCTGCCAGTTGAAGAACTGT2402132.852053402937717No Hit
CCCCCACTGCTAAATTTGACTG1564181.8571538142428254No Hit
TATTGCACTTGTCCCGGCCTGTAA1258471.4941837644070175No Hit
CGCCTGTCTGAGCGTCGCT1256701.4920822401251512No Hit
TATTGCACTTGTCCCGGCCTGT1163181.3810457707239383No Hit
TATTGCACTTGTCCCGGCCTGTAT1062141.2610807913794286No Hit
TGAGGTAGTAGGTTGTATAGTT840570.9980103195527955No Hit
TAGCTTATCAGACTGATGTTGACT624390.7413394047200947No Hit
TAGCTTATCAGACTGATGTTGA607820.7216658129966336No Hit
TAGCTTATCAGACTGATGTTGAC569470.6761327868895279No Hit
TATTGCACTTGTCCCGGCCTGTA567730.6740668816632864No Hit
TCGTACGACTCTTAGCGG551640.6549631948298228No Hit
CATAAAGTAGAAAGCACTACT531460.6310034434128374No Hit
ACCGGGTGCTGTAGGCTT518530.6156516304385252No Hit
TTCTCACTACTGCACTTGACTAG429640.5101123686220816No Hit
TGTAAACATCCTTGACTGGAAGCT422100.5011601126417015No Hit
TGAGGTAGTAGATTGTATAGTT404530.4802992190640784No Hit
AGCAGCATTGTACAGGGCTATGA393400.4670845494272575No Hit
ACTGGACTTGGAGTCAGAAGGC392790.46636029529621875No Hit
ACCGGGTGCTGTAGGCTTA376690.44724473544421356No Hit
CAACGGAATCCCAAAAGCAGCTGT357250.4241635873993079No Hit
TTCAAGTAATTCAGGATAGGTT346270.41112701304061117No Hit
CTCCCACTGCTTCACTTGACTAG335140.3979123434037902No Hit
AACATTCAACGCTGTCGGTGAGTT331770.3939111361552649No Hit
GCCTGTCTGAGCGTCGCT330300.392165802429647No Hit
TGTAAACATCCCCGACTGGAAGCT323760.3844008483034288No Hit
ATCCCGGACGAGCCCCCA309670.3676717651782888No Hit
AGCTCGGTCTGAGGCCCCTCAGT305200.3623645258901855No Hit
ACCGGGTGCTGTAGGCTTT303320.3601323984043613No Hit
CCCATAAAGTAGAAAGCACT266440.3163447060228736No Hit
TACGCCTGTCTGAGCGTCGCT266430.316332833004332No Hit
CATTGCACTTGTCTCGGTCTGA265090.3147418485197552No Hit
CTTCTCACTACTGCACTTGACTAG256000.30394927466542426No Hit
CTACGCCTGTCTGAGCGTCGCT254390.3020377186802238No Hit
CAACACCAGTCGATGGGCTGT247010.2932754309965096No Hit
ACGCCTGTCTGAGCGTCGCT241470.2866977787244531No Hit
CCCATAAAGTAGAAAGCACTAC224690.2667748536116179No Hit
AACATTCAACGCTGTCGGTGA189720.22525490777157925No Hit
TGAGGTAGTAGTTTGTACAGTT184900.21953211283451934No Hit
TATTGCACTTGTCCCGGCCTGTT167490.19886118755356214No Hit
AATGACACGATCACTCCCGTTG163800.19448004371170508No Hit
CGCGACCTCAGATCAGACGTGG162510.19294842431983633No Hit
TATTGCACTTGTCCCGGCCTGTAG154270.18316505704154298No Hit
TGAGGTAGTAGGTTGTGTGGTT151520.179899976942598No Hit
CGCGACCTCAGATCAGACGTGGCG151260.17959127846051592No Hit
AACCGGGCGGAAACACCA149970.17805965906864718No Hit
CACTAGATTGTGAGCTCCTGGA142050.1686562283836856No Hit
TGAGGTAGTAGTTTGTGCTGTT140910.16730270426994115No Hit
AACGGAATCCCAAAAGCAGCTG140220.1664834659905695No Hit
CAAAGTGCTGTTCGTGCAGGT138810.16480937037620136No Hit
CACCCGGCTGTGTGCACATGTGC137640.163420227206832No Hit
TTCACAGTGGCTAAGTTCCG136290.16181736970371358No Hit
TGAGAACTGAATTCCATGGGTT135660.16106936953559164No Hit
GGAATACCGGGTGCTGTAGGCTT130310.15471730461582592No Hit
AACATTCAACGCTGTCGGTGAGA129460.15370809803978838No Hit
ATCCCACCGCTGCCACCA125000.1484127317702267No Hit
TTCACAGTGGCTAAGTTCTG123420.14653679484065105No Hit
TTCAAGTAATCCAGGATAGGC119800.14223876212858527No Hit
TAGCAGCACGTAAATATTGGC119110.14141952384921364No Hit
CCCCCCACTGCTAAATTTGACTGG118850.14111082536713154No Hit
GGAATACCGGGTGCTGTAGGCTTT118170.14030346010630151No Hit
TGTAAACATCCTACACTCTCAGCT114490.13593418928298603No Hit
CGCGACCTCAGATCAGACGTGGC111030.13182612486758616No Hit
TTCAAGTAATCCAGGATAGGCTT110490.13118498186633878No Hit
TGTAAACATCCCCGACTGGAAGC109140.12958212436322034No Hit
GCGTTCGCGCTTTCCCCTG108660.12901221947322267No Hit
ACCACAGGGTAGAACCACGGAC108320.12860853684280765No Hit
CGCGACCTCAGATCAGACGT106630.1266019967092742No Hit
TTAATGCTAATCGTGATAGGGGTT103740.12317069435074654No Hit
TCCCACTGCTTCACTTGACTAG103350.12270764662762343No Hit
CACTAGATTGTGAGCTCCTGGAA100140.11889640767576402No Hit
AACATTCATTGCTGTCGGTGGGT98390.11681862943098084No Hit
AACATTCATTGCTGTCGGTGGG96950.11510891476098782No Hit
TCGTTTCCCGGGCGGCGCACCA95580.11348231122078614No Hit
TATTGCACTTGTCCCGGCCTGA95310.11316173972016245No Hit
TATTGCACTTGTCCCGGCCTG95060.11286491425662198No Hit
ACTGGACTTGGAGTCAGAAGGCT94100.11172510447662665No Hit
CTCCCACTGCTTCACTTGACTAGCCT93290.1107633899747556No Hit
ACCCCCCACTGCTAAATTTGACTG92500.10982542150996775No Hit
TGATGTAGTAGGTTGTATAGTT90410.10734396063476956No Hit
CCCCCACTGCTAAATTTGACTGGCTT88200.10472002353707195No Hit
ACTGGACTTGGAGTCAGAAGG88200.10472002353707195No Hit
TAATGTGAATTGCAGGACA87930.10439945203644828No Hit
TTATAAAGCAATGAGACTGATT87050.10335462640478586No Hit
CCCCCCACTGCTAAATTTGACTGGCT86350.1025235151068726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)