FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9473125
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG146363315.450371445536714No Hit
CAACGGAATCCCAAAAGCAGCTG8889049.383429438543248No Hit
CCCCCACTGCTAAATTTGACTG3728273.9356284225110514No Hit
TTCAAGTAATCCAGGATAGGCT3045533.2149158804512767No Hit
AACATTCAACGCTGTCGGTGAGT3005943.1731239691231776No Hit
TAGCAGCACGTAAATATTGGCG2759172.912629148248334No Hit
AACATTCAACGCTGTCGGTGAG2584852.7286138417892722No Hit
AAGCTGCCAGTTGAAGAACTGT2242932.3676769809329024No Hit
TATTGCACTTGTCCCGGCCTGTAA1576511.6641921224516725No Hit
TAGCTTATCAGACTGATGTTGACT1487971.5707277165666027No Hit
TATTGCACTTGTCCCGGCCTGTAT1413441.4920525169888499No Hit
TAGCTTATCAGACTGATGTTGAC1312391.3853823315959624No Hit
TAGCTTATCAGACTGATGTTGA997751.0532427261331399No Hit
TGAGGTAGTAGGTTGTATAGTT867250.9154845945767632No Hit
TGTAAACATCCTTGACTGGAAGCT815930.8613102856765851No Hit
GGAATACCGGGTGCTGTAGGCTTT704000.7431549778980009No Hit
TATTGCACTTGTCCCGGCCTGTA623780.6584733126608168No Hit
TATTGCACTTGTCCCGGCCTGT623380.6580510655142838No Hit
TTCAAGTAATTCAGGATAGGTT581060.6133773174110971No Hit
AGCAGCATTGTACAGGGCTATGA549430.5799881242990037No Hit
TGAGGTAGTAGATTGTATAGTT542810.5729999340238833No Hit
CAACGGAATCCCAAAAGCAGCT541450.5715642937256713No Hit
CAACGGAATCCCAAAAGCAGCTGT497260.5249165402124432No Hit
CATAAAGTAGAAAGCACTACT450770.47584086560665034No Hit
GGAATACCGGGTGCTGTAGGCTT446940.47179784917859735No Hit
TTCTCACTACTGCACTTGACTAG409940.432739988124299No Hit
TGTAAACATCCCCGACTGGAAGCT368820.3893329814607112No Hit
CCCCCACTGCTAAATTTGACTGGCTT353880.37356205053770536No Hit
CTCCCACTGCTTCACTTGACTAG352240.37183083723692023No Hit
CCCATAAAGTAGAAAGCACTAC341610.36060961931780694No Hit
ACCCCCCACTGCTAAATTTGACTG328120.34636933430098304No Hit
CTTCTCACTACTGCACTTGACTAG304180.32109784258098567No Hit
TGAATACCGGGTGCTGTAGGCTTT301310.31806821930461177No Hit
CAAAGAATTCTCCTTTTGGGCT292520.30878933825955No Hit
CCCCCCACTGCTAAATTTGACTGG277430.2928600646565943No Hit
TGTAAACATCCTACACTCTCAGCT276200.2915616546810055No Hit
CATTGCACTTGTCTCGGTCTGA269210.28418288579534207No Hit
TAGCTTATCAGACTGATGTTGACA250720.2646645114468562No Hit
TGAGGTAGTAGTTTGTACAGTT218370.2305152734710035No Hit
ACTGGACTTGGAGTCAGAAGGC210840.2225664709375206No Hit
TCGTTTCCCGGCCAATGCACCA198050.20906511842712938No Hit
CCCCCCACTGCTAAATTTGACTGGCT196780.20772448373688723No Hit
TGAATACCGGGTGCTGTAGGCTT189280.19980734973939432No Hit
CCCATAAAGTAGAAAGCACT185670.19599656924193443No Hit
AACCGGGCGGAAACACCA182160.19229135053110774No Hit
TATTGCACTTGTCCCGGCCTGTAG176120.18591541861846012No Hit
TGATGTAGTAGGTTGTATAGTT169080.1784838688394801No Hit
AACGGAATCCCAAAAGCAGCTG167230.1765309757867652No Hit
CTCCCACTGCTTCACTTGACTAGCCT167020.1763092960348354No Hit
TAGCAGCACGTAAATATTGGC163800.1729102065052451No Hit
AACATTCAACGCTGTCGGTGAGTT161800.17079897077258033No Hit
CACCCGGCTGTGTGCACATGTGC155880.1645497130038926No Hit
TATTGCACTTGTCCCGGCCTGTT154920.1635363198522135No Hit
CAACGGAATCCCAAAAGCAGCTGA154810.16342020188691694No Hit
TTCAAGTAATCCAGGATAGGCTT146860.15502803984957444No Hit
TGAGGTAGTAGTTTGTGCTGTT146300.15443689384442832No Hit
CAACACCAGTCGATGGGCTGT145360.15344461305007587No Hit
TTCACAGTGGCTAAGTTCCG135870.14342679949858153No Hit
AGCAGCATTGTACAGGGCTATGT133950.14140001319522333No Hit
CCCCCCACTGCTAAATTTGACTGT132880.14027050207824768No Hit
TATTGCACTTGTCCCGGCCTGTTT132540.13991159200369466No Hit
GCCTGTCTGAGCGTCGCT132230.13958435046513162No Hit
ATCACATTGCCAGGGATTTCC131660.13898264828132215No Hit
TTCACAGTGGCTAAGTTCTGC130030.13726199115920037No Hit
TTCAAGTAATCCAGGATAGGC129850.13707197994326054No Hit
CCCCCCACTGCTAAATTTGACTGTA129110.13629082272217458No Hit
CACTAGATTGTGAGCTCCTGGAA128240.1353724351784654No Hit
CAAAGAATTCTCCTTTTGGGCTT126610.13365177805634362No Hit
TTCACAGTGGCTAAGTTCCGC125880.13288117701392096No Hit
TATTGCACTTGTCCCGGCCTGTAAT124670.1316038793956588No Hit
CACTAGATTGTGAGCTCCTGGA119680.12633634624266016No Hit
GGAATACCGGGTGCTGTAGGCTTA117760.12430955993930197No Hit
TATTGCACTTGTCCCGGCCTGTAAA113990.12032988058322887No Hit
AGCTCGGTCTGAGGCCCCTCAGT113400.11970706604209275No Hit
TAGCAGCACATCATGGTTTACA111940.11816586395724747No Hit
AACATTCAACGCTGTCGGTGAGA110060.11618130236854259No Hit
TGTCAGTTTGTCAAATACCCCA109630.11572738668601966No Hit
CTACGCCTGTCTGAGCGTCGCT109630.11572738668601966No Hit
TGTCAGTTTGTCAAATACCCCAA106900.11284554991093225No Hit
ATGCACCTGGGCAAGGATTCTGA103520.10927756152272877No Hit
TGATGTAGTAGATTGTATAGTT102270.10795803918981328No Hit
TGTAAACATCCCCGACTGGAAGC99190.10470673616150954No Hit
TACGCCTGTCTGAGCGTCGCT98390.10386224186844362No Hit
AATGCACCCGGGCAAGGATTCT97390.10280662400211124No Hit
ATCACATTGCCAGGGATTTCCA95670.10099096127201954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)