FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5358204
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4439778.285929389773141No Hit
CAACGGAATCCCAAAAGCAGCTG2993725.587170626575622No Hit
TTCAAGTAATCCAGGATAGGCT2220624.1443364231746305No Hit
CCCCCACTGCTAAATTTGACTG2001493.735374763633486No Hit
AACATTCAACGCTGTCGGTGAGT1813933.3853321000842818No Hit
TAGCAGCACGTAAATATTGGCG1580462.9496077416985242No Hit
AAGCTGCCAGTTGAAGAACTGT1393772.6011887565311063No Hit
AACATTCAACGCTGTCGGTGAG1357152.532844960736844No Hit
TGAGGTAGTAGGTTGTATAGTT791621.4773980236661388No Hit
TAGCTTATCAGACTGATGTTGA703441.312827955038666No Hit
CATAAAGTAGAAAGCACTACT555821.0373251932923795No Hit
TAGCTTATCAGACTGATGTTGAC552341.0308304797652348No Hit
ACCGGGTGCTGTAGGCTT543021.0134365918132269No Hit
TAGCTTATCAGACTGATGTTGACT494660.9231824693498045No Hit
TATTGCACTTGTCCCGGCCTGT491830.9179008488665232No Hit
TGAGGTAGTAGATTGTATAGTT441320.8236341878733993No Hit
ACCGGGTGCTGTAGGCTTT390570.7289196156025415No Hit
TATTGCACTTGTCCCGGCCTGTAA369810.6901752900785412No Hit
TATTGCACTTGTCCCGGCCTGTAT347220.6480156410618184No Hit
ACCGGGTGCTGTAGGCTTA340960.6363326219009205No Hit
GCCTGTCTGAGCGTCGCT327350.6109323198594155No Hit
CCCATAAAGTAGAAAGCACT323360.6034857948670861No Hit
TCGTACGACTCTTAGCGG320650.5984281300226718No Hit
TTCAAGTAATTCAGGATAGGTT320100.5974016666778645No Hit
CTCCCACTGCTTCACTTGACTAG307210.5733450984695618No Hit
CGCCTGTCTGAGCGTCGCT298450.5569963368322669No Hit
TTCTCACTACTGCACTTGACTAG266370.4971255293751414No Hit
TGTAAACATCCTTGACTGGAAGCT254210.4744313579699466No Hit
CCCATAAAGTAGAAAGCACTAC252950.4720798237618426No Hit
TATTGCACTTGTCCCGGCCTGTA242350.45229707566191957No Hit
CATTGCACTTGTCTCGGTCTGA235930.4403154489825322No Hit
CAACGGAATCCCAAAAGCAGCT213480.3984170815444877No Hit
TGAGGTAGTAGTTTGTACAGTT189520.3537006056506994No Hit
CAACACCAGTCGATGGGCTGT187800.35049057482693824No Hit
AGCAGCATTGTACAGGGCTATGA180590.3370345735250095No Hit
CAACGGAATCCCAAAAGCAGCTGT170390.31799834422130996No Hit
ATCCCGGACGAGCCCCCA169390.3161320472307512No Hit
TCGTTTCCCGGCCAATGCACCA157830.29455765401989176No Hit
TTCACAGTGGCTAAGTTCCG143240.26732838092763916No Hit
TTCAAGTAATCCAGGATAGGC139170.25973255217606495No Hit
CTTCTCACTACTGCACTTGACTAG132450.24719103639951No Hit
TCCCACTGCTTCACTTGACTAG132300.2469110918509262No Hit
GGAATACCGGGTGCTGTAGGCTTT130030.24267459768235775No Hit
TGTAAACATCCTACACTCTCAGCT123510.23050634130391454No Hit
TGAGGTAGTAGTTTGTGCTGTT113490.21180604545851556No Hit
CCCCCCACTGCTAAATTTGACTGG112800.21051830053503No Hit
ACTGGACTTGGAGTCAGAAGGC111870.20878264433381039No Hit
TGTAAACATCCCCGACTGGAAGCT110610.2064311101257063No Hit
GGAATACCGGGTGCTGTAGGCTT107200.20006703738790088No Hit
AACATTCAACGCTGTCGGTGA105450.19680101765442304No Hit
TTCACAGTGGCTAAGTTCTG103230.19265783833538253No Hit
TGAGGTAGTAGGTTGTATAGT97730.18239320488730926No Hit
AACCGGGCGGAAACACCA95570.1783620033877023No Hit
TTCACAGTGGCTAAGTTCTGC91620.17099013027499513No Hit
TGTAAACATCCTACACTCAGCT88990.16608176918982553No Hit
AACGGAATCCCAAAAGCAGCTG88310.16481268723624556No Hit
TCCTGTCTGAGCGTCGCT87260.16285307539615887No Hit
CTACGCCTGTCTGAGCGTCGCT86840.16206923066012419No Hit
TCCACCACGTTCCCGTGG85600.15975502239183131No Hit
CCCCCACTGCTAAATTTGACTGGCTT85070.15876588498683514No Hit
CAAAGTGCTGTTCGTGCAGGT84630.15794471431098928No Hit
ATCCCACCGCTGCCACCA81910.1528683864966694No Hit
TAGCAGCACGTAAATATTGGC79480.14833328480961158No Hit
AACATTCAACGCTGTCGGTGAGTT77600.1448246464673611No Hit
TTAATGCTAATCGTGATAGGGGTT76960.14363021639340345No Hit
AATGACACGATCACTCCCGTTG75230.14040152259973676No Hit
ACCCCCCACTGCTAAATTTGACTG73920.13795667354210478No Hit
TTCAAGTAATCCAGGATAGGCTT71970.13431739441051518No Hit
TTCACAGTGGCTAAGTTCCGC71920.13422407956098723No Hit
AGAATTACTCTGAGACCT71790.13398146095221458No Hit
CCCATAAAGTAGAAAGCACTA71390.13323494215599108No Hit
TATTGCACTTGTCCCGGCCTGTT71340.13314162730646315No Hit
CACCCGGCTGTGTGCACATGTGC71310.13308563839674636No Hit
TTATAAAGCAATGAGACTGATT70840.13220847881118375No Hit
TGATGTAGTAGGTTGTATAGTT70090.13080875606826467No Hit
ACGCCTGTCTGAGCGTCGCT69060.12888647016798913No Hit
TGAGGTAGTAGGTTGTGTGGTT67880.12668423971912976No Hit
TAGCAGCACATCATGGTTTACA67230.12547114667526654No Hit
TGTCAGTTTGTCAAATACC67060.12515387618687157No Hit
TGTCAGTTTGTCAAATACCCCA66360.12384746829348042No Hit
CCCCCCACTGCTAAATTTGACTGGCT63980.11940568145595053No Hit
TACGCCTGTCTGAGCGTCGCT62860.1173154288265247No Hit
TGAGGTAGTAGATTGTATAGT62670.11696083239831855No Hit
TGTCAGTTTGTCAAATACCCCAA61850.11543046886606034No Hit
AGCTCGGTCTGAGGCCCCTCAGT61660.11507587243785418No Hit
TCAGTGCACTACAGAACTTTGT61250.11431069067172508No Hit
CTCCCACTGCTTCACTTGACTA60680.11324690138710658No Hit
CAAAGAATTCTCCTTTTGGGCT59880.11175386379465954No Hit
CCCCCCACTGCTAAATTTGACTGTA59740.11149258221598132No Hit
AGAGGTAGTAGGTTGCATAGTT59330.11072740044985223No Hit
TGAGAACTGAATTCCATGGGTT59170.11042879293136282No Hit
CTGCTGTGATGACATTCC58490.10915971097778285No Hit
CTCCCACTGCTTCACTTGACTAGCCT57080.10652823222109499No Hit
ATCACATTGCCAGGGATTTCC55950.10441931662176357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)