FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10412896
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG174845916.791284576356087No Hit
CAACGGAATCCCAAAAGCAGCTG5770195.541388293900179No Hit
AACATTCAACGCTGTCGGTGAGT4428414.252813050279192No Hit
CCCCCACTGCTAAATTTGACTG4351254.178712627111612No Hit
AACATTCAACGCTGTCGGTGAG2910202.7948036742132065No Hit
TTCAAGTAATCCAGGATAGGCT2280172.18975585658399No Hit
TAGCAGCACGTAAATATTGGCG1901391.8259953811120364No Hit
AAGCTGCCAGTTGAAGAACTGT1775431.705029993577195No Hit
TATTGCACTTGTCCCGGCCTGTAA1135041.09003297449624No Hit
CGCCTGTCTGAGCGTCGCT1128901.0841364400451132No Hit
TATTGCACTTGTCCCGGCCTGTAT967320.9289634699126927No Hit
TATTGCACTTGTCCCGGCCTGT896710.8611533237247351No Hit
TGAGGTAGTAGGTTGTATAGTT847760.8141443072129021No Hit
ACCGGGTGCTGTAGGCTT786420.7552365835594632No Hit
TATTGCACTTGTCCCGGCCTGTA673800.6470822334151806No Hit
CAACGGAATCCCAAAAGCAGCT645970.6203557588590148No Hit
ACCGGGTGCTGTAGGCTTT642120.6166584204816796No Hit
TAGCTTATCAGACTGATGTTGAC627940.6030406910815205No Hit
ACCGGGTGCTGTAGGCTTA607160.5830846673202152No Hit
TAGCTTATCAGACTGATGTTGACT565570.5431438093686904No Hit
TAGCTTATCAGACTGATGTTGA556690.5346159224100577No Hit
CTCCCACTGCTTCACTTGACTAG526270.5054021474909574No Hit
TCGTTTCCCGGCCAATGCACCA486440.4671515013690715No Hit
GCCTGTCTGAGCGTCGCT464830.44639838907447077No Hit
CCCCCCACTGCTAAATTTGACTGG401950.3860117300700977No Hit
TGAGGTAGTAGATTGTATAGTT371310.3565866786722925No Hit
CTACGCCTGTCTGAGCGTCGCT369580.35492527727156786No Hit
CATAAAGTAGAAAGCACTACT359070.34483202367525806No Hit
AGCAGCATTGTACAGGGCTATGA352000.338042365927788No Hit
TACGCCTGTCTGAGCGTCGCT334190.3209385746289985No Hit
GCGTTCGCGCTTTCCCCTG316430.30388280071173285No Hit
TTCTCACTACTGCACTTGACTAG316010.30347945470693266No Hit
ACGCCTGTCTGAGCGTCGCT306390.2942409105017471No Hit
TTAATGCTAATCGTGATAGGGGTT301570.2896120349228495No Hit
CAACGGAATCCCAAAAGCAGCTGT293530.2818908399738171No Hit
TGTAAACATCCTTGACTGGAAGCT279860.26876288786520103No Hit
ACTGGACTTGGAGTCAGAAGGC255620.24548406130244652No Hit
TTCAAGTAATTCAGGATAGGTT243530.23387345844998356No Hit
AACATTCAACGCTGTCGGTGAGTT239150.22966713582849574No Hit
CTTCTCACTACTGCACTTGACTAG236140.22677648946076095No Hit
CCCCCACTGCTAAATTTGACTGGCTT232430.2232135997516925No Hit
ACCCCCCACTGCTAAATTTGACTG229710.22060145419679597No Hit
CCCATAAAGTAGAAAGCACTAC226060.21709618534555614No Hit
CATTGCACTTGTCTCGGTCTGA224970.2160494064283366No Hit
CCCCCCACTGCTAAATTTGACTGTA224750.2158381299496317No Hit
CCCATAAAGTAGAAAGCACT217260.20864512619736142No Hit
TGTAAACATCCCCGACTGGAAGCT214980.20645553359987462No Hit
CCCCCCACTGCTAAATTTGACTGT202980.19493136203415456No Hit
CCCCCCACTGCTAAATTTGACTGGCT202540.19450880907674484No Hit
AGCTCGGTCTGAGGCCCCTCAGT197140.1893229318721708No Hit
CAACACCAGTCGATGGGCTGT190080.18254287760100552No Hit
TCCACCACGTTCCCGTGG184260.1769536543916313No Hit
CACCCGGCTGTGTGCACATGTGC178040.1709802921300664No Hit
GACCCGCGGGCGCTCTCCAGTCCT177850.17079782608027586No Hit
TCGTACGACTCTTAGCGG172380.16554472454156846No Hit
CTCCCACTGCTTCACTTGACTAGCCT172180.1653526550154731No Hit
ATCCCGGACGAGCCCCCA159130.15282011843775256No Hit
TGAGGTAGTAGTTTGTACAGTT157650.1513988039446471No Hit
TATTGCACTTGTCCCGGCCTGTT153140.1470676361311973No Hit
TGTAAACATCCTACACTCTCAGCT150880.14489725048632005No Hit
TGAGGTAGTAGGTTGTGTGGTT148190.1423139153603378No Hit
TATTGCACTTGTCCCGGCCTGTAG147490.14164167201900413No Hit
TAGCAGCACATCATGGTTTACA146570.1407581521989656No Hit
AACGGAATCCCAAAAGCAGCTG142770.13710883120315426No Hit
GGAATACCGGGTGCTGTAGGCTTT141630.13601403490441083No Hit
TTCACAGTGGCTAAGTTCCG139670.13413175354867657No Hit
AACATTCAACGCTGTCGGTGA136610.13119308979941793No Hit
TCCCACTGCTTCACTTGACTAG134120.12880182419953104No Hit
CACTAGATTGTGAGCTCCTGGA131080.12588236740288195No Hit
CAACGGAATCCCAAAAGCAGCTGA127980.12290528974840428No Hit
TCGTTTCCCGGGCGGCGCACCA126570.12155119958943217No Hit
GCTTGACTAGCTTGCTGTTT125570.12059085195895551No Hit
TGAGGTAGTAGTTTGTGCTGTT122770.11790187859362082No Hit
CCCCCCACTGCTAAATTTGACTGA121620.11679747881857265No Hit
CCTCGACACAAGGGTTTGT121360.11654778843464873No Hit
GGAATACCGGGTGCTGTAGGCTT120750.11596197638005797No Hit
TTCAAGTAATCCAGGATAGGC119660.11491519746283838No Hit
ATCCCACCGCTGCCACCA114190.10966209592413101No Hit
GTCAGGATGGCCGAGCGGTCTAAGG105840.1016431932096508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)