FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9118435
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103363011.33560748088899No Hit
CAACGGAATCCCAAAAGCAGCTG6722327.37222999341444No Hit
CCCCCACTGCTAAATTTGACTG3261233.576523822344514No Hit
AACATTCAACGCTGTCGGTGAGT3138723.442169626695809No Hit
TTCAAGTAATCCAGGATAGGCT2813793.0858255830084875No Hit
TAGCAGCACGTAAATATTGGCG2575542.8245417113792004No Hit
AAGCTGCCAGTTGAAGAACTGT2522232.766077731540555No Hit
AACATTCAACGCTGTCGGTGAG2494342.7354913425384946No Hit
TATTGCACTTGTCCCGGCCTGTAA1302201.428095939708952No Hit
TATTGCACTTGTCCCGGCCTGTAT1196921.3126375304534166No Hit
TAGCTTATCAGACTGATGTTGA1158591.2706018083146944No Hit
TAGCTTATCAGACTGATGTTGACT1134701.2444021369895162No Hit
TAGCTTATCAGACTGATGTTGAC1006591.1039065365931764No Hit
TCGTACGACTCTTAGCGG949311.0410887394602255No Hit
TGAGGTAGTAGGTTGTATAGTT930871.0208659709697991No Hit
TATTGCACTTGTCCCGGCCTGT890550.9766478567868281No Hit
CATAAAGTAGAAAGCACTACT762910.8366676957175218No Hit
TATTGCACTTGTCCCGGCCTGTA688830.7554256843416661No Hit
TGTAAACATCCTTGACTGGAAGCT620890.6809172845998244No Hit
TGAGGTAGTAGATTGTATAGTT611000.6700711251437336No Hit
TTCAAGTAATTCAGGATAGGTT556690.6105104658858675No Hit
TTCTCACTACTGCACTTGACTAG452000.495699097487672No Hit
AGCAGCATTGTACAGGGCTATGA428590.47002583228371975No Hit
CCCATAAAGTAGAAAGCACT406670.4459866194144061No Hit
CAACGGAATCCCAAAAGCAGCTGT401510.4403277536112283No Hit
CCCATAAAGTAGAAAGCACTAC364370.39959707998137833No Hit
TGTAAACATCCCCGACTGGAAGCT362400.397436621525514No Hit
CTCCCACTGCTTCACTTGACTAG336280.36879135509547417No Hit
CATTGCACTTGTCTCGGTCTGA320000.35093741415056423No Hit
CAACGGAATCCCAAAAGCAGCT312280.34247104903418185No Hit
CTTCTCACTACTGCACTTGACTAG301200.3303198410692186No Hit
ACCGGGTGCTGTAGGCTT254240.27881977554262327No Hit
TGAGGTAGTAGTTTGTACAGTT244110.26771041302591947No Hit
ACCGGGTGCTGTAGGCTTA235700.258487339110275No Hit
CAACACCAGTCGATGGGCTGT232700.25519730085261344No Hit
TGTAAACATCCTACACTCTCAGCT230830.2531465103386711No Hit
CCCCCACTGCTAAATTTGACTGGCTT225090.2468515704723453No Hit
ACTGGACTTGGAGTCAGAAGGC223920.2455684555518573No Hit
ACCCCCCACTGCTAAATTTGACTG221250.24264032150253853No Hit
CCCCCCACTGCTAAATTTGACTGG204430.22419417367124952No Hit
TTCACAGTGGCTAAGTTCCG203140.22277945722045506No Hit
ACCGGGTGCTGTAGGCTTT201210.22066286594135945No Hit
CAAAGAATTCTCCTTTTGGGCT201050.22048739723428418No Hit
TCGTTTCCCGGCCAATGCACCA194410.21320544589065998No Hit
AACATTCAACGCTGTCGGTGAGTT184870.20274312423129628No Hit
TATTGCACTTGTCCCGGCCTGTT184270.20208511657976397No Hit
CGCCTGTCTGAGCGTCGCT173040.18976940670191758No Hit
GCCTGTCTGAGCGTCGCT172070.1887056276652737No Hit
ATCCCGGACGAGCCCCCA161520.1771356597924973No Hit
TGAGGTAGTAGTTTGTGCTGTT159260.17465716430505893No Hit
AACGGAATCCCAAAAGCAGCTG154720.1696782397417978No Hit
CCCCCCACTGCTAAATTTGACTGTA151520.16616886560029215No Hit
AGCTCGGTCTGAGGCCCCTCAGT150940.16553279153714426No Hit
TATTGCACTTGTCCCGGCCTGTAG147130.1613544429499141No Hit
TAGCTTATCAGACTGATGTTGACA146850.16104737271253236No Hit
CCCATAAAGTAGAAAGCACTA144790.1587882131089381No Hit
TACGCCTGTCTGAGCGTCGCT144330.15828374057609668No Hit
CCCCCCACTGCTAAATTTGACTGGCT143290.15714319398010734No Hit
TTCAAGTAATCCAGGATAGGC143180.15702255924399308No Hit
TAGCAGCACGTAAATATTGGC141060.1546975988752456No Hit
CACCCGGCTGTGTGCACATGTGC140950.15457696413913133No Hit
TCAGTGCACTACAGAACTTTGT140820.15443439581463267No Hit
TTCACAGTGGCTAAGTTCTG135090.14815042274249912No Hit
CACTAGATTGTGAGCTCCTGGA128560.14098910613498916No Hit
TTCAAGTAATCCAGGATAGGCTT123320.1352425059782737No Hit
CCCCCCACTGCTAAATTTGACTGT122910.13479286741639326No Hit
CTACGCCTGTCTGAGCGTCGCT121060.13276401049083533No Hit
CACTAGATTGTGAGCTCCTGGAA120360.13199633489738097No Hit
AACATTCAACGCTGTCGGTGA117730.12911206802483102No Hit
TGTAAACATCCCCGACTGGAAGC113810.12481308470148661No Hit
TATTGCACTTGTCCCGGCCTGTTT111180.12192881782893666No Hit
AGCAGCATTGTACAGGGCTATGT106130.11639058676187305No Hit
TGATGTAGTAGGTTGTATAGTT103740.11376952294993603No Hit
TGTCAGTTTGTCAAATACCCCA102740.11267284353071551No Hit
TATTGCACTTGTCCCGGCCTGA99160.10874673120990609No Hit
TCGTTTCCCGGGCGGCGCACCA98060.10754038384876352No Hit
AACATTCAACGCTGTCGGTGAGA96930.10630113610504434No Hit
TGTCAGTTTGTCAAATACCCCAA96270.1055773276883588No Hit
AATGACACGATCACTCCCGTTG95950.10522639027420824No Hit
TTATAAAGCAATGAGACTGATT95540.10477675171232782No Hit
TGAGGTAGTAGGTTGTATAGT95110.104305179562063No Hit
CAAAGTGCTGTTCGTGCAGGT91860.10074097144959634No Hit
CAAAGAATTCTCCTTTTGGGCTT91720.10058743633090546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)