FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6862371
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5746118.373359586650153No Hit
AAGCTGCCAGTTGAAGAACTGT3163014.609208683121329No Hit
TTCAAGTAATCCAGGATAGGCT2598933.787218732417702No Hit
AACATTCAACGCTGTCGGTGAGT2399543.496663179533721No Hit
TAGCAGCACGTAAATATTGGCG2373373.4585276721413054No Hit
CCCCCCACTGCTAAATTTGACTG2170903.1634838745966953No Hit
GCCTGTCTGAGCGTCGCT1695482.470691252338295No Hit
AACATTCAACGCTGTCGGTGAG1523542.220136451381017No Hit
TAGCTTATCAGACTGATGTTGAC1226311.7870062694074687No Hit
TGAGGTAGTAGGTTGTATAGTT1167951.701962776422318No Hit
TAGCTTATCAGACTGATGTTGACT1083731.5792355149553996No Hit
CGCCTGTCTGAGCGTCGCT1033581.5061558169909497No Hit
TAGCTTATCAGACTGATGTTGA1010701.4728145709405684No Hit
CATAAAGTAGAAAGCACTACT989881.4424752028125556No Hit
CCCCCACTGCTAAATTTGACTG784641.1433948995179655No Hit
TATTGCACTTGTCCCGGCCTGT722901.0534259951844631No Hit
TGAGGTAGTAGATTGTATAGTT676210.985388286351758No Hit
TATTGCACTTGTCCCGGCCTGTAA663900.9674498799321692No Hit
TGTAAACATCCTTGACTGGAAGCT613550.8940787375092369No Hit
TATTGCACTTGTCCCGGCCTGTAT577630.8417353127658065No Hit
CAACACCAGTCGATGGGCTGT541330.7888381435512595No Hit
TCGTACGACTCTTAGCGG507610.7397006078511349No Hit
TTCAAGTAATTCAGGATAGGTT502930.7328808075226478No Hit
AGCAGCATTGTACAGGGCTATGA496910.724108329322329No Hit
AACCGGGCGGAAACACCA490440.7146801010904248No Hit
TCCTGTCTGAGCGTCGCT450720.6567992316358297No Hit
CCCATAAAGTAGAAAGCACT410170.5977088676785327No Hit
CCCATAAAGTAGAAAGCACTAC410170.5977088676785327No Hit
ACTGGACTTGGAGTCAGAAGGC372240.5424364261273545No Hit
TATTGCACTTGTCCCGGCCTGTA370270.5395656982113034No Hit
TGAGGTAGTAGTTTGTACAGTT367140.5350045924360545No Hit
CAACGGAATCCCAAAAGCAGCTGT345940.5041114798369252No Hit
CATTGCACTTGTCTCGGTCTGA308780.44996109945090407No Hit
TGTAAACATCCCCGACTGGAAGCT302810.44126148236520585No Hit
TGTCAGTTTGTCAAATACCCCAA277770.4047726361632153No Hit
ATCCCGGACGAGCCCCCA249390.3634166675045695No Hit
TACGCCTGTCTGAGCGTCGCT248120.36156599519320654No Hit
TGAGGTAGTAGTTTGTGCTGTT215380.3138565373396454No Hit
ACGCCTGTCTGAGCGTCGCT209250.3049237646871613No Hit
ATCCCACCGCTGCCACCA203480.29651559205994543No Hit
TGTAAACATCCTACACTCTCAGCT199620.29089071401123606No Hit
TTCACAGTGGCTAAGTTCCGC195470.2848432414977272No Hit
AACGGAATCCCAAAAGCAGCTG189000.2754150132658231No Hit
CTACGCCTGTCTGAGCGTCGCT178380.2599393125204102No Hit
TTCACAGTGGCTAAGTTCCG177260.2583072235529091No Hit
ACCGGGTGCTGTAGGCTT170270.2481212397289508No Hit
AACATTCAACGCTGTCGGTGAGTT169430.24689717300332495No Hit
TTATAAAGCAATGAGACTGATT153550.22375648299982615No Hit
TAGCAGCACGTAAATATTGGC144080.20995658789068677No Hit
CAACGGAATCCCAAAAGCAGCT140370.20455029318583912No Hit
TTCAAGTAATCCAGGATAGGCTT139530.20332622646021323No Hit
TGTCAGTTTGTCAAATACCCCA136740.1992605762643844No Hit
CACCCGGCTGTGTGCACATGTGC130880.1907212536308515No Hit
TTCAAGTAATCCAGGATAGGC124120.18087043093414798No Hit
TGAGGTAGTAGGTTGTGTGGTT123240.1795880753168256No Hit
ACCACAGGGTAGAACCACGGAC121980.1777519752283868No Hit
TGAGGTAGTAGGTTGTATAGT121570.177154514088498No Hit
ACCGGGTGCTGTAGGCTTT119970.17482295842063916No Hit
AGCAGCATTGTACAGGGCTATGT117090.17062615821849328No Hit
GCGTTCGCGCTTTCCCCTG115500.16830917477355858No Hit
TAGCTTATCAGACTGATGTTGACA114490.1668373802582227No Hit
TAGCAGCACGTAAATATTGGCGT113700.1656861746472174No Hit
CAAAGTGCTGTTCGTGCAGGT110840.16151851889091975No Hit
TTCACAGTGGCTAAGTTCTGC109210.1591432465542886No Hit
CAACACCAGTCGATGGGCTGTC108160.15761316314725626No Hit
TATTGCACTTGTCCCGGCCTGTT108030.15742372424924272No Hit
ACTGGACTTGGAGTCAGAAGG107730.1569865575615192No Hit
ACTGGACTTGGAGTCAGAAGGCA106110.15462585744781213No Hit
TGTAAACATCCTACACTCAGCT105210.15331435738464155No Hit
AGCAGCATTGTACAGGGCTATG102670.1496130127619157No Hit
ACTGGACTTGGAGTCAGAAGGCT98670.1437841235922686No Hit
ACCGGGTGCTGTAGGCTTA97140.14155457348487863No Hit
CTCCCACTGCTTCACTTGACTAG95210.1387421344605239No Hit
TTCACAGTGGCTAAGTTCTG94090.13711004549302275No Hit
AACATTCAACGCTGTCGGTGA92500.13479306204808805No Hit
TGTAAACATCCCCGACTGGAAGC90560.13196605080080923No Hit
TTCTCACTACTGCACTTGACTAG89240.1300425173748257No Hit
CCCATAAAGTAGAAAGCACTA82210.119798244659171No Hit
CAAAGAATTCTCCTTTTGGGCT81660.11899677239834454No Hit
TTAGGTAGTAGGTTGTATAGTT81270.11842845570430396No Hit
AGAGGTAGTAGGTTGCATAGTT81000.11803500568535277No Hit
CAAAGAATTCTCCTTTTGGGCTT80130.11676722229095454No Hit
CAAAGTGCTGTTCGTGCAGGTAG77130.11239555541371926No Hit
AATGACACGATCACTCCCGTTG76100.11089461645253515No Hit
TAGCAGCACATAATGGTTTGT75540.11007857196878455No Hit
TGAGGTAGTAGATTGTATAGT73950.10776158852384984No Hit
CTTCTCACTACTGCACTTGACTAG73650.10732442183612632No Hit
CCCCCACTGCTAAATTTGACTGGCTT71440.10410396056989633No Hit
TCGTTTCCCGGCCAATGCACCA71000.10346278276123515No Hit
ATCACATTGCCAGGGATTTCC70590.10286532162134632No Hit
TACCTGGTTGATCCTGCC70160.10223871603560926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)