FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5766725
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3725186.459784366343115No Hit
AAGCTGCCAGTTGAAGAACTGT2819994.890106602967889No Hit
AACATTCAACGCTGTCGGTGAGT2031203.5222765087636394No Hit
TTCAAGTAATCCAGGATAGGCT1826173.166736752662907No Hit
TAGCAGCACGTAAATATTGGCG1807533.134413380211472No Hit
CGCCTGTCTGAGCGTCGCT1443982.503986231353151No Hit
CCCCCCACTGCTAAATTTGACTG1411112.4469868079369137No Hit
AACATTCAACGCTGTCGGTGAG1361722.361340275459641No Hit
TAGCTTATCAGACTGATGTTGAC1155312.0034074799821386No Hit
GCCTGTCTGAGCGTCGCT784501.3603908630982056No Hit
TAGCTTATCAGACTGATGTTGACT781271.3547897636873616No Hit
TATTGCACTTGTCCCGGCCTGTAA661821.1476531306764237No Hit
TGAGGTAGTAGGTTGTATAGTT642041.1133528996093969No Hit
TATTGCACTTGTCCCGGCCTGT639701.1092951371879185No Hit
TAGCTTATCAGACTGATGTTGA636741.104162241133399No Hit
TGTAAACATCCTTGACTGGAAGCT623781.0816884800298263No Hit
TATTGCACTTGTCCCGGCCTGTAT552400.9579093853096863No Hit
CATAAAGTAGAAAGCACTACT538070.9330599256943933No Hit
CCCCCACTGCTAAATTTGACTG507550.8801356055646836No Hit
AGCAGCATTGTACAGGGCTATGA471450.8175350827376023No Hit
ACTGGACTTGGAGTCAGAAGGC391910.679605842137435No Hit
TGAGGTAGTAGATTGTATAGTT381410.6613979338359295No Hit
TCCTGTCTGAGCGTCGCT358800.6221902379600206No Hit
TATTGCACTTGTCCCGGCCTGTA350550.6078840242945519No Hit
TACGCCTGTCTGAGCGTCGCT341860.5928148125669249No Hit
TTAATGCTAATCGTGATAGGGGTT329700.5717283206672765No Hit
TTCAAGTAATTCAGGATAGGTT321040.5567111315347966No Hit
TGTAAACATCCCCGACTGGAAGCT310860.5390581309148607No Hit
CAACACCAGTCGATGGGCTGT275680.4780529676722924No Hit
ACGCCTGTCTGAGCGTCGCT271720.47118598511286736No Hit
AACCGGGCGGAAACACCA261240.4530127585414598No Hit
GCGTTCGCGCTTTCCCCTG254660.441602469339183No Hit
CCCATAAAGTAGAAAGCACTAC250520.43442335120887504No Hit
CCCATAAAGTAGAAAGCACT247770.4296546133203855No Hit
TGTCAGTTTGTCAAATACCCCAA230520.39974162111076916No Hit
CAACGGAATCCCAAAAGCAGCTGT216610.37562047782753644No Hit
CATTGCACTTGTCTCGGTCTGA210590.3651812770680065No Hit
CTACGCCTGTCTGAGCGTCGCT200920.3484126605655723No Hit
TCGTACGACTCTTAGCGG196150.340141067937174No Hit
TGAGGTAGTAGTTTGTACAGTT191550.3321642700146097No Hit
ATCCCGGACGAGCCCCCA176580.3062049950361774No Hit
TGTAAACATCCTACACTCTCAGCT167640.290702261682324No Hit
AACATTCAACGCTGTCGGTGAGTT159720.2769682965634741No Hit
ACCGGGTGCTGTAGGCTT146460.2539743095084298No Hit
TTCACAGTGGCTAAGTTCCG133160.2309109589931894No Hit
TTATAAAGCAATGAGACTGATT124650.21615388283644532No Hit
TAGCAGCACGTAAATATTGGC124590.216049837646151No Hit
CCTCGACACAAGGGTTTGT124480.2158590881306114No Hit
TACCTGGTTGATCCTGCC121700.21103832764697467No Hit
TGAGGTAGTAGTTTGTGCTGTT121590.21084757813143506No Hit
TCGTTCGCGCTTTCCCCTG120520.20899210557118642No Hit
TGTCAGTTTGTCAAATACCCCA119720.2076048363672622No Hit
ATCCCACCGCTGCCACCA119270.2068244974400548No Hit
CAACGGAATCCCAAAAGCAGCT119160.2066337479245152No Hit
TTCAAGTAATCCAGGATAGGCTT117060.2029921662642141No Hit
AACGGAATCCCAAAAGCAGCTG114730.19895174470778476No Hit
CCAAGTCCTTCTGATCGAGGCCC112450.19499802747660067No Hit
ACCACAGGGTAGAACCACGGAC111060.1925876472347823No Hit
CACCCGGCTGTGTGCACATGTGC110610.19180730830757492No Hit
ACTGGACTTGGAGTCAGAAGGCA110600.19178996744252588No Hit
TAGCTTATCAGACTGATGTTGACA109660.19015992612791488No Hit
ACTGGACTTGGAGTCAGAAGG109630.19010790353276774No Hit
TTCACAGTGGCTAAGTTCCGC107170.1858420507307007No Hit
AGCAGCATTGTACAGGGCTATGT103140.17885368211593236No Hit
TGTAAACATCCTACACTCAGCT100410.17411962595754088No Hit
CAAGTCCTTCTGATCGAGGCCC98950.17158785966037918No Hit
ACCGGGTGCTGTAGGCTTT98870.17144913273998671No Hit
TGAGAACTGAATTCCATAGGCTGT97820.16962834190983617No Hit
TAGCAGCACGTAAATATTGGCGT96110.16666305398644812No Hit
ACTGGACTTGGAGTCAGAAGGCT91150.15806198492211784No Hit
TTCAAGTAATCCAGGATAGGC90660.15721228253471425No Hit
TATTGCACTTGTCCCGGCCTGTT90290.15657067052789927No Hit
TGTAAACATCCCCGACTGGAAGC84690.14685978610042963No Hit
TCGTTTCCCGGCCAATGCACCA84630.1467557409101353No Hit
AGCAGCATTGTACAGGGCTATG82700.14340895395566808No Hit
CAAAGAATTCTCCTTTTGGGCTT82400.1428887280041965No Hit
TGAGGTAGTAGGTTGTGTGGTT81110.14065175641286867No Hit
ACCGGGTGCTGTAGGCTTA80250.1391604420186501No Hit
CTGCGTTCGCGCTTTCCCCTG80160.13900437423320863No Hit
CAAAGAATTCTCCTTTTGGGCT79640.13810264925065788No Hit
CAAAGTGCTGTTCGTGCAGGT77880.13505065700202454No Hit
AGCTCGGTCTGAGGCCCCTCAGT77010.13354200174275693No Hit
CTCGACACAAGGGTTTGT76860.13328188876702113No Hit
CACCCGTAGAACCGACCTTGCG74870.1298310566222596No Hit
TTCACAGTGGCTAAGTTCTGC74430.12906805856010128No Hit
TTCACAGTGGCTAAGTTCTG73880.12811431098240336No Hit
TATTGCACTTGTCCCGGCCTGTAG73410.12729929032509787No Hit
CAACACCAGTCGATGGGCTGTC70690.12258257503175547No Hit
CAAAGTGCTGTTCGTGCAGGTAG69930.12126466928802744No Hit
CTCCTGTCTGAGCGTCGCT67840.11764042849277535No Hit
TGAGGTAGTAGGTTGTATAGT67560.11715488427140187No Hit
AACATTCAACGCTGTCGGTGA66960.1161144323684587No Hit
ATCACATTGCCAGGGATTTCC65260.1131664853101197No Hit
AACATTCAACGCTGTCGGTGAGA63140.10949022191972047No Hit
AATGACACGATCACTCCCGTTG62150.10777347627986422No Hit
TTCACCACCTTCTCCACCCAGC61340.10636886621089092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)