FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966237
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG77047211.06008882557398No Hit
CCCCCCACTGCTAAATTTGACTG3649935.239457112929118No Hit
AACATTCAACGCTGTCGGTGAGT2563283.679576218839525No Hit
TTCAAGTAATCCAGGATAGGCT2323703.335660271104759No Hit
TAGCAGCACGTAAATATTGGCG1990822.8578126182040604No Hit
AAGCTGCCAGTTGAAGAACTGT1881722.7012000883690863No Hit
AACATTCAACGCTGTCGGTGAG1816402.6074335398006125No Hit
TATTGCACTTGTCCCGGCCTGTAA1279511.8367305045751385No Hit
TATTGCACTTGTCCCGGCCTGTAT1115101.600720733446192No Hit
TAGCTTATCAGACTGATGTTGACT964511.3845495064264968No Hit
TAGCTTATCAGACTGATGTTGAC948161.361079159379734No Hit
CCCCCACTGCTAAATTTGACTG897061.2877253530133987No Hit
TAGCTTATCAGACTGATGTTGA716401.028388784360911No Hit
TTAATGCTAATCGTGATAGGGGTT673040.9661457110919425No Hit
TATTGCACTTGTCCCGGCCTGT623650.8952466015726999No Hit
AGCAGCATTGTACAGGGCTATGA578540.83049141164735No Hit
TATTGCACTTGTCCCGGCCTGTA567020.8139545065721996No Hit
TGTAAACATCCTTGACTGGAAGCT549470.7887615652467752No Hit
CATAAAGTAGAAAGCACTACT499180.7165705100185366No Hit
CAACGGAATCCCAAAAGCAGCTGT468320.6722711271522918No Hit
CCCATAAAGTAGAAAGCACTAC454340.6522029038058854No Hit
TGAGGTAGTAGGTTGTATAGTT419730.6025204138188235No Hit
TGTAAACATCCCCGACTGGAAGCT402240.5774136022073323No Hit
ACTGGACTTGGAGTCAGAAGGC385310.553110667925883No Hit
TCGTACGACTCTTAGCGG380300.5459188368124713No Hit
TTCAAGTAATTCAGGATAGGTT333640.47893862927718367No Hit
CAAAGAATTCTCCTTTTGGGCT322630.46313382677046444No Hit
CAACGGAATCCCAAAAGCAGCT318890.45776507460196947No Hit
TGAGGTAGTAGATTGTATAGTT294340.4225236666510198No Hit
CCCATAAAGTAGAAAGCACT293770.42170543436865554No Hit
AACCGGGCGGAAACACCA281240.40371867910896514No Hit
ATCCCGGACGAGCCCCCA258870.3716066507642505No Hit
TGTAAACATCCTACACTCTCAGCT234070.3360063690052463No Hit
CATTGCACTTGTCTCGGTCTGA226660.32536934933451156No Hit
CCCCCACTGCTAAATTTGACTGGCTT217910.3128087660526049No Hit
AACATTCAACGCTGTCGGTGAGTT206080.295826857455467No Hit
CGCCTGTCTGAGCGTCGCT192770.27672041591464663No Hit
AGCTCGGTCTGAGGCCCCTCAGT186290.26741840680987455No Hit
CACCCGGCTGTGTGCACATGTGC178560.25632202866483006No Hit
CAACACCAGTCGATGGGCTGT177280.25448459476759117No Hit
TCGTTTCCCGGGCGGCGCACCA166810.23945495968626965No Hit
TTCACAGTGGCTAAGTTCCG164450.23606719093823536No Hit
CAAAGAATTCTCCTTTTGGGCTT164050.2354929928453482No Hit
ACTGGACTTGGAGTCAGAAGGCA155770.2236070923225839No Hit
TCGTTTCCCGGCCAATGCACCA154960.22244434118448742No Hit
TATTGCACTTGTCCCGGCCTGTAG152180.21845366443892159No Hit
CTACGCCTGTCTGAGCGTCGCT152100.21833882482034417No Hit
TAGCTTATCAGACTGATGTTGACA149670.21485057140605465No Hit
AACGGAATCCCAAAAGCAGCTG142300.20427097154460866No Hit
AGCTACATCTGGCTACTGGGTCTC142000.20384032297494328No Hit
TATTGCACTTGTCCCGGCCTGTT136010.195241706533958No Hit
GCCTGTCTGAGCGTCGCT133970.19231329626023347No Hit
ACTGGACTTGGAGTCAGAAGGCT130700.1876192268508809No Hit
CCCATAAAGTAGAAAGCACTA129780.18629857123724042No Hit
TAGCAGCACGTAAATATTGGC128390.18430323286445752No Hit
CCCCCCACTGCTAAATTTGACTGGCT126300.18130304782912207No Hit
ACCGGGTGCTGTAGGCTTA123900.17785785927179912No Hit
CTCCCACTGCTTCACTTGACTAG123110.17672381803834697No Hit
TTCAAGTAATCCAGGATAGGCTT120760.17335040424263487No Hit
CACTAGATTGTGAGCTCCTGGAA119360.17134071091752978No Hit
TGAGAACTGAATTCCATAGGCTGT117830.1691444032122364No Hit
AGCAGCATTGTACAGGGCTATGT116250.1668763207453321No Hit
GGAATACCGGGTGCTGTAGGCTTT116070.16661793160353286No Hit
TGTCAGTTTGTCAAATACCCCAA113810.1633737123787204No Hit
CTTCTCACTACTGCACTTGACTAG113720.16324451780782076No Hit
TTAATGCTAATCGTGATAGGGGTTT113700.16321580790317644No Hit
TATTGCACTTGTCCCGGCCTGTAAA113260.16258419000100055No Hit
TGAGGTAGTAGTTTGTACAGTT109200.15675607935819583No Hit
TGTAAACATCCCCGACTGGAAGC106280.15256443328011954No Hit
CACTAGATTGTGAGCTCCTGGA106170.15240652880457556No Hit
TTCTCACTACTGCACTTGACTAG105760.15181797575936623No Hit
TATTGCACTTGTCCCGGCCTGTAAT104980.15069828947823624No Hit
CAACGGAATCCCAAAAGCAGCTGA103800.1490044051042191No Hit
TATTGCACTTGTCCCGGCCTGTTT101390.14554486159457394No Hit
TTCAAGTAATCCAGGATAGGC99430.14273129093942685No Hit
TACGCCTGTCTGAGCGTCGCT95910.13767834772201978No Hit
ACCCCCCACTGCTAAATTTGACTG94710.1359557534433583No Hit
AGCTACATTGTCTGCTGGGTTT94260.13530978058886023No Hit
TGAGAACTGAATTCCATGGGTT93120.13367331602413182No Hit
ACCGGGTGCTGTAGGCTT92970.13345799173929915No Hit
ATCACATTGCCAGGGATTTCC90590.1300415130866205No Hit
TGTCAGTTTGTCAAATACCCCA89530.12851988814046952No Hit
ACCACAGGGTAGAACCACGGAC88960.1277016558581053No Hit
ACTGGACTTGGAGTCAGAAGG88400.1268977785280633No Hit
CCCCCCACTGCTAAATTTGACTGG88030.12636664529214267No Hit
ACCGGGTGCTGTAGGCTTT87670.1258498670085442No Hit
CACCCGTAGAACCGACCTTGCG86510.12418469253917144No Hit
ATCACATTGCCAGGGATTTCCA85530.12277790721159788No Hit
TGATGTAGTAGGTTGTATAGTT83450.11979207712858463No Hit
CTCCCACTGCTTCACTTGACTAGCCT83000.11914610427408658No Hit
TGAGGTAGTAGTTTGTGCTGTT82420.11831351703940017No Hit
AACATTCAACGCTGTCGGTGAGA82030.11775367389883519No Hit
ATGCACCTGGGCAAGGATTCTGA82010.11772496399419083No Hit
AGCAGCATTGTACAGGGCTATG81290.11669140742699394No Hit
TTCACAGTGGCTAAGTTCTG81140.11647608314216125No Hit
AACATTCAACGCTGTCGGTGA80810.11600236971552934No Hit
GGAATACCGGGTGCTGTAGGCTT79620.11429413038919004No Hit
ACTTGACTTGGAGTCAGAAGGC78720.1130021846801939No Hit
ATACCGGGTGCTGTAGGCTTA78680.1129447648709052No Hit
TTAATGCTAATCGTGATAGGGGT77790.11166717411423126No Hit
TAGCAGCACATCATGGTTTACA76710.1101168392634359No Hit
CAAAGTGCTGTTCGTGCAGGTAG76280.10949957631358222No Hit
TATTGCACTTGTCCCGGCCTGA75170.10790617660582033No Hit
ACGCCTGTCTGAGCGTCGCT73130.10497776633209578No Hit
CAAAGTGCTGTTCGTGCAGGT71380.10246564967571443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)