FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8092926
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG151135518.675013215245016No Hit
CCCCCCACTGCTAAATTTGACTG3419464.225245603382509No Hit
TTCAAGTAATCCAGGATAGGCT3132943.871208015494025No Hit
TAGCAGCACGTAAATATTGGCG2545543.145388948323511No Hit
AACATTCAACGCTGTCGGTGAG2237242.7644389680567945No Hit
TAGCTTATCAGACTGATGTTGAC1787472.2086820020348634No Hit
TCGTACGACTCTTAGCGG1710592.1136854581396145No Hit
AAGCTGCCAGTTGAAGAACTGT1706042.108063264139571No Hit
TGAGGTAGTAGATTGTATAGTT1402381.732846686105866No Hit
CCCCCACTGCTAAATTTGACTG1221401.5092192860777425No Hit
TAGCTTATCAGACTGATGTTGA1170531.4463619215102177No Hit
TAGCTTATCAGACTGATGTTGACT1152651.4242685525605943No Hit
TATTGCACTTGTCCCGGCCTGTAA959361.1854303375565276No Hit
TGAGGTAGTAGGTTGTATAGTT841981.0403900888257225No Hit
TATTGCACTTGTCCCGGCCTGT751870.929045934684192No Hit
TATTGCACTTGTCCCGGCCTGTAT735760.9091396609829374No Hit
AACATTCAACGCTGTCGGTGAGT731710.9041352904993818No Hit
TGTAAACATCCTTGACTGGAAGCT712710.8806579968728245No Hit
CAACGGAATCCCAAAAGCAGCTGT690590.8533254845033799No Hit
AGCAGCATTGTACAGGGCTATGA571880.7066418252187157No Hit
CATAAAGTAGAAAGCACTACT567290.7009702053373527No Hit
CAACGGAATCCCAAAAGCAGCT521060.6438462430028398No Hit
TGAGGTAGTAGTTTGTACAGTT514720.6360122408137675No Hit
TTCAAGTAATTCAGGATAGGTT505530.6246566445807117No Hit
TATTGCACTTGTCCCGGCCTGTA477580.5901203100090128No Hit
TGTAAACATCCCCGACTGGAAGCT408430.5046753176786739No Hit
CCCCCACTGCTAAATTTGACTGGCTT399510.4936533461445218No Hit
CCCATAAAGTAGAAAGCACT388700.4802960017180436No Hit
AACGGAATCCCAAAAGCAGCTG380970.4707444501531337No Hit
CCCATAAAGTAGAAAGCACTAC379870.46938523841685936No Hit
TGAGAACTGAATTCCATGGGTT360710.4457102412650258No Hit
CATTGCACTTGTCTCGGTCTGA358340.442781757796871No Hit
TCGTTTCCCGGCCAATGCACCA344040.4251120052253042No Hit
ACTGGACTTGGAGTCAGAAGGC334170.4129161690098242No Hit
TAGCTTATCAGACTGATGTTGACA300500.3713119334094986No Hit
CAAAGAATTCTCCTTTTGGGCT295560.3652078370665937No Hit
TGAGAACTGAATTCCATAGGCTGT268000.33115340483775585No Hit
TGAGGTAGTAGTTTGTGCTGTT266060.328756249593781No Hit
GCCTGTCTGAGCGTCGCT261250.3228127873651631No Hit
TAGCAGCACGTAAATATTGGC258240.31909348979590324No Hit
CACCCGGCTGTGTGCACATGTGC257040.3176107133563312No Hit
TTCTCACTACTGCACTTGACTAG253110.31275462051673275No Hit
TTCACAGTGGCTAAGTTCCGC237810.29384922091218924No Hit
CCCATAAAGTAGAAAGCACTA230360.2846436505165128No Hit
TTAATGCTAATCGTGATAGGGGTT228980.282938457611005No Hit
GTCAGGATGGCCGAGCGGTCTAAGG228140.2819005141033045No Hit
TGTAAACATCCTACACTCTCAGCT217220.26840724850319897No Hit
CAACACCAGTCGATGGGCTGT212950.26313103567238844No Hit
CTCCCACTGCTTCACTTGACTAG210300.2598565710350002No Hit
TGTCAGTTTGTCAAATACCCCA189930.23468644097326483No Hit
CTTCTCACTACTGCACTTGACTAG173120.21391521434892644No Hit
CTCCCACTGCTTCACTTGACTAGCCT161050.19900095466089768No Hit
TTCACAGTGGCTAAGTTCTGC154010.19030199954874172No Hit
TGAGGTAGTAGATTGTATAGT152250.18812726077070271No Hit
AATGCACCCGGGCAAGGATTCT150320.18574246199705768No Hit
ACCAGGATGGCCGAGTGGTTAAGGC147620.1824062150080206No Hit
TGTAAACATCCCCGACTGGA147390.18212201619043594No Hit
AATCCTTGGAACCTAGGTGTGAGT144970.17913175037063234No Hit
ATCACATTGCCAGGGATTTCC144830.1789587597860156No Hit
GGAATACCGGGTGCTGTAGGCTTT140040.1730400104980572No Hit
CAAAGAATTCTCCTTTTGGGCTT134680.16641694240130206No Hit
ACTGGACTTGGAGTCAGAAGGCT134230.16586090123646255No Hit
TTCAAGTAATCCAGGATAGGC133090.1644522636188691No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC132770.16405685656831656No Hit
TTCACAGTGGCTAAGTTCCG131940.1630312695309459No Hit
CCCCCCACTGCTAAATTTGACTGGCT130850.16168441426500132No Hit
TTCACAGTGGCTAAGTTCTG128550.1588424260891549No Hit
ATGCACCTGGGCAAGGATTCTGA125300.15482657323198062No Hit
TGATGTAGTAGATTGTATAGTT123170.15219464505174024No Hit
TATTGCACTTGTCCCGGCCTGTT122000.1507489380231575No Hit
CAAAGTGCTGTTCGTGCAGGTAG120290.14863598159676736No Hit
CAAAGAATTCTCCTTTTGGGC117330.14497846637915632No Hit
CACCCGTAGAACCGACCTTGCG117320.14496610990882655No Hit
AGCAGCATTGTACAGGGCTATGT116710.14421236521871075No Hit
ACTGGACTTGGAGTCAGAAGGCA116110.14347097699892475No Hit
TGAGGTAGTAGTTTGTGCTGTTA115810.14310028288903173No Hit
TTCAAGTAATCCAGGATAGGCTT114510.14149394174616203No Hit
TAGCAGCACATCATGGTTTACA113360.14007294765823883No Hit
TGTAAACATCCCCGACTGGAAGC112580.13910914297251697No Hit
AACCGGGCGGAAACACCA107570.1329185513373037No Hit
ACCCCCCACTGCTAAATTTGACTG106140.13115157608014702No Hit
TCGTTTCCCGGCCAACGCACCA105780.1307067431482754No Hit
TGGCTCAGTTCAGCAGGAAC105770.13069438667794564No Hit
GGAATACCGGGTGCTGTAGGCTT104140.12868028201419363No Hit
TGAGGTAGTAGATTGTATAGTTA100640.12435551739877519No Hit
TGTCAGTTTGTCAAATACCCCAA98650.12189657980315155No Hit
TATTGCACTTGTCCCGGCCTGTAG98300.1214641033416097No Hit
CAACGGAATCCCAAAAGCAGCTGA97480.12045087277456881No Hit
AGCTCGGTCTGAGGCCCCTCAGT96330.1190298786866456No Hit
TGAGGTAGTAAGTTGTATTGTT93880.11600254345585269No Hit
TATTGCACTTGTCCCGGCCTGTAAA89590.11070161768438264No Hit
CCCCCCACTGCTAAATTTGACTGG87400.10799555068216365No Hit
ACTGGACTTGGAGTCAGAAGG87280.10784727303820646No Hit
TAGCAGCACGTAAATATTGGCGT87230.10778549068655761No Hit
CACTAGATTGTGAGCTCCTGGAA86090.10637685306896419No Hit
AGCAGCATTGTACAGGGCTATG81760.10102650141617506No Hit
GTCAGGATGGCCGAGTGGTCTAAGG81220.10035925201836765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)