FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9318402
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG164778917.68317142789075No Hit
CCCCCACTGCTAAATTTGACTG8463519.08257660487281No Hit
TAGCTTATCAGACTGATGTTGA4817255.169609553225971No Hit
TAGCAGCACGTAAATATTGGCG2900313.112454259861294No Hit
AAGCTGCCAGTTGAAGAACTGT2174582.333640467539391No Hit
AACATTCAACGCTGTCGGTGAG2130882.286744014692648No Hit
CAACGGAATCCCAAAAGCAGCTG1997102.143178626549917No Hit
TAGCTTATCAGACTGATGTTGACT1894062.032601727206017No Hit
TTCAAGTAATCCAGGATAGGCT1762821.8917621283134167No Hit
TAGCTTATCAGACTGATGTTGAC1709661.8347137202279964No Hit
CATAAAGTAGAAAGCACTACT1585531.7015041849450154No Hit
AACATTCAACGCTGTCGGTGAGT970051.0410046701140387No Hit
GGAATACCGGGTGCTGTAGGCTTT911790.9784832206208747No Hit
TGTAAACATCCTTGACTGGAAGCT860380.923312816940072No Hit
TGAGGTAGTAGATTGTATAGTT819010.8789167928149054No Hit
CCCCCACTGCTAAATTTGACTGGCTT743390.7977655396279318No Hit
TGAGGTAGTAGGTTGTATAGTT708110.7599049708308355No Hit
TTCTCACTACTGCACTTGACTAG692710.7433785320701983No Hit
TATTGCACTTGTCCCGGCCTGTAA670550.7195976305808657No Hit
GGAATACCGGGTGCTGTAGGCTT607660.6521075180057696No Hit
TTCAAGTAATTCAGGATAGGTT570630.6123689448040555No Hit
TATTGCACTTGTCCCGGCCTGTA559370.600285327892057No Hit
CCCATAAAGTAGAAAGCACT499250.5357678280031275No Hit
CAAAGAATTCTCCTTTTGGGCT478780.5138005421959688No Hit
TAGCTTATCAGACTGATGTTG447790.48054376705362145No Hit
TTCACAGTGGCTAAGTTCCG443100.4755107152492455No Hit
TATTGCACTTGTCCCGGCCTGT420720.4514937217776181No Hit
CAACACCAGTCGATGGGCTGT413660.44391731543670254No Hit
CCCCCCACTGCTAAATTTGACTGG372530.39977884620131215No Hit
CCCCCACTGCTAAATTTGACTGGCTA349900.37549356638616793No Hit
CTCCCACTGCTTCACTTGACTAG336070.3606519658628164No Hit
TGAGGTAGTAGTTTGTACAGTT334040.35847348075345964No Hit
TATTGCACTTGTCCCGGCCTGTAT312300.33514330032123535No Hit
ACCCCCCACTGCTAAATTTGACTG298180.3199904876394043No Hit
CCCCCCACTGCTAAATTTGACTGA295380.31698568059201565No Hit
TGTCAGTTTGTCAAATACC275460.2956086247405939No Hit
TAGCTTATCAGACTGATGTTGACA272190.2920994393673937No Hit
AGCAGCATTGTACAGGGCTATGA270010.2897599824519268No Hit
TGAGGTAGTAGTTTGTGCTGTT265840.28528496624206595No Hit
CCCCCCACTGCTAAATTTGACTGTA249800.26807171444202554No Hit
CCCCCCACTGCTAAATTTGACTGGCT247720.26583957206396547No Hit
CTTCTCACTACTGCACTTGACTAG240040.25759781559112815No Hit
TGAGAACTGAATTCCATGGGTT228830.2455678559478331No Hit
CATTGCACTTGTCTCGGTCTGA221320.23750853418858728No Hit
TGTAAACATCCCCGACTGGAAGCT219600.2356627241451914No Hit
TTCACAGTGGCTAAGTTCTG218820.23482567075341887No Hit
CCCCCACTGCTAAATTTGACTGG218580.2345681158636427No Hit
CCCCCACTGCTAAATTTGACTGGCT209260.22456640097733496No Hit
TAGCAGCACGTAAATATTGGC193440.20758924115958938No Hit
AGAATTACTCTGAGACCT187750.2014830439811461No Hit
GCCTGTCTGAGCGTCGCT181470.19474369103200312No Hit
CCCCCACAACCGCGCTTGACTAG176530.18944235288411038No Hit
TGAGGTAGTAGATTGTATAGT175280.18810092116652619No Hit
CCCATAAAGTAGAAAGCACTA167700.17996647923109563No Hit
CCCCCACTGCTAAATTTGACTGA166590.17877528786588084No Hit
ACGTGGGCACATTACCCGTCTGACC161080.17286225685476975No Hit
CCCCCCACTGCTAAATTTGACTGT159360.1710164468113739No Hit
TGAGGTAGTAGGTTGTATAGT158720.17032963377197077No Hit
GGAATACCGGGTGCTGTAGGCTTA158350.16993256998356587No Hit
TGTCAGTTTGTCAAATACCCCA157930.16948184892645754No Hit
CACTAGATTGTGAGCTCCTGGAA157790.16933160857408813No Hit
CCCCCACTGCTAAATTTGACTGTA157510.16903112786934926No Hit
TTCAAGTAATCCAGGATAGGC153000.1641912422323055No Hit
TTAATGCTAATCGTGATAGGGGTT152190.16332199447931095No Hit
TGTAAACATCCTACACTCTCAGCT151230.16229177492020627No Hit
GACCCGCGGGCGCTCTCCAGTCCT139580.1497896313123216No Hit
TGTCAGTTTGTCAAATACCCCAA128120.13749138532550967No Hit
CACTAGATTGTGAGCTCCTGGA128050.13741626514932495No Hit
CAAAGAATTCTCCTTTTGGGC127260.13656848030381175No Hit
ACTGGACTTGGAGTCAGAAGG125950.1351626598637835No Hit
AGCAGCATTGTACAGGGCTATG125780.13498022515019206No Hit
TATTGCACTTGTCCCGGCCTGTT125040.1341860975733822No Hit
CCCCCACTGCTAAATTTGACTGGA124940.13407878303597548No Hit
TCGTACGACTCTTAGCGG121910.1308271525525514No Hit
TATTGCACTTGTCCCGGCCTG121520.13040862585666513No Hit
CGGGCCGCCGGTGAAATACCACTAC120590.1294106006587825No Hit
GGAATACCGGGTGCTGTAGGCT119560.1283052609234931No Hit
CCCCCCACTGCTAAATTTGACTGGA116910.1254614256822146No Hit
CCCCCCACAACCGCGCTTGACTAG115740.1242058455945558No Hit
TCAGTGCACTACAGAACTTTGT109490.11749868700663482No Hit
TGAATACCGGGTGCTGTAGGCTTT108970.1169406514121198No Hit
TAGCTTATCAGACTGATGTTGAA108880.11684406832845375No Hit
ACTGGACTTGGAGTCAGAAGGC105650.11337780877021617No Hit
TCCCACTGCTTCACTTGACTAG103760.11134956401322887No Hit
TGAGAACTGAATTCCATAGGCTGT102980.11051251062145634No Hit
AGTGTTTCCTACTTTATGGATG102790.11030861300038355No Hit
CCCCCCACTGCTAAATTTGAC100440.10778672137132525No Hit
TCGTTTCCCGGGCGGCGCACCA97810.1049643490375281No Hit
CCCCCACTGCTAAATTTGACTGT96750.10382681494101671No Hit
TTCAAGTAATTCAGGATAGGT94590.10150882093303121No Hit
ATCCCGGACGAGCCCCCA94510.10142296930310583No Hit
TTATAAAGCAATGAGACTGATT94120.10100444260721957No Hit
GTGGGCACATTACCCGTCTGACC93860.10072542480996205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)