FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9012577
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG136502115.145734677218293No Hit
CCCCCACTGCTAAATTTGACTG7543788.370280775409741No Hit
AACATTCAACGCTGTCGGTGAG3465363.8450267886754257No Hit
TAGCTTATCAGACTGATGTTGA3188623.5379669987840328No Hit
CAACGGAATCCCAAAAGCAGCTG3049923.38407094885292No Hit
AAGCTGCCAGTTGAAGAACTGT2558912.8392656173700375No Hit
TAGCAGCACGTAAATATTGGCG2461732.73143852196769No Hit
TTCAAGTAATCCAGGATAGGCT1905052.1137683483869263No Hit
AACATTCAACGCTGTCGGTGAGT1837342.0386400027428335No Hit
TAGCTTATCAGACTGATGTTGACT1631831.8106142116733095No Hit
TAGCTTATCAGACTGATGTTGAC1425881.582100213956563No Hit
CATAAAGTAGAAAGCACTACT1120301.2430406974608927No Hit
CCCATAAAGTAGAAAGCACT1108171.229581727845432No Hit
TATTGCACTTGTCCCGGCCTGTAA941411.0445514085482988No Hit
TGTAAACATCCTTGACTGGAAGCT754270.8369082450002924No Hit
TATTGCACTTGTCCCGGCCTGTA749320.831415920219045No Hit
CCCCCACTGCTAAATTTGACTGGCTT630280.69933383093426No Hit
CAACACCAGTCGATGGGCTGT618490.6862521119098345No Hit
TGAGGTAGTAGGTTGTATAGTT598390.6639499446162845No Hit
GGAATACCGGGTGCTGTAGGCTTT580290.643866898446471No Hit
TATTGCACTTGTCCCGGCCTGT552780.6133428873894781No Hit
TGAGGTAGTAGATTGTATAGTT419940.46594886235091254No Hit
TTCTCACTACTGCACTTGACTAG410980.4560071997165739No Hit
CAAAGAATTCTCCTTTTGGGCT398450.44210440587636585No Hit
GGAATACCGGGTGCTGTAGGCTT387190.4296107539497305No Hit
TATTGCACTTGTCCCGGCCTGTAT374490.41551933481400494No Hit
TTAATGCTAATCGTGATAGGGGTT347230.385272713897479No Hit
TTCAAGTAATTCAGGATAGGTT337720.3747207929541129No Hit
AGCAGCATTGTACAGGGCTATGA321230.35642413928890704No Hit
CAACGGAATCCCAAAAGCAGCT308000.34174465305539137No Hit
CCCCCCACTGCTAAATTTGACTGTA298810.33154779149182306No Hit
TTCACAGTGGCTAAGTTCCG294610.32688763713197677No Hit
CCCATAAAGTAGAAAGCACTA290690.3225381597294536No Hit
TGTAAACATCCCCGACTGGAAGCT275720.30592803811828734No Hit
TCGTACGACTCTTAGCGG271520.3012678837584411No Hit
ACCCCCCACTGCTAAATTTGACTG264690.2936895851208816No Hit
CCCCCACTGCTAAATTTGACTGGCTA261590.2902499473790903No Hit
CCCCCCACTGCTAAATTTGACTGG253720.281517705757188No Hit
TAGCTTATCAGACTGATGTTGACA245890.2728298465577603No Hit
CATTGCACTTGTCTCGGTCTGA243320.26997827591375917No Hit
TAGCTTATCAGACTGATGTTG240990.2673929998046064No Hit
CTCCCACTGCTTCACTTGACTAG224580.24918511098435No Hit
CCCCCCACTGCTAAATTTGACTGA217610.24145147386812893No Hit
TGAGAACTGAATTCCATGGGTT212750.2360590095374497No Hit
CCCCCACTGCTAAATTTGACTGTA206070.22864714498417046No Hit
TGAGGTAGTAGTTTGTACAGTT202230.22438643242659673No Hit
CCCCCCACTGCTAAATTTGACTGGCT199380.2212241848252725No Hit
TCGTTTCCCGGCCAATGCACCA195140.21651964804295154No Hit
TGAGGTAGTAGTTTGTGCTGTT186590.20703290523897883No Hit
TGTAAACATCCTACACTCTCAGCT183190.2032603993286271No Hit
TTCACAGTGGCTAAGTTCTG181820.20174030135886772No Hit
TATTGCACTTGTCCCGGCCTG171090.18983471653002243No Hit
TAGCAGCACGTAAATATTGGC170140.1887806339962477No Hit
CTTCTCACTACTGCACTTGACTAG169070.18759340419504877No Hit
CCCCCACTGCTAAATTTGACTGGCT168160.18658370408374875No Hit
CACTAGATTGTGAGCTCCTGGAA167750.1861287842533828No Hit
CCCATAAAGTAGAAAGCACTAC160680.17828419108097496No Hit
TTCAAGTAATCCAGGATAGGC160270.17782927125060902No Hit
AGCAGCATTGTACAGGGCTATG153810.17066151002094074No Hit
TATTGCACTTGTCCCGGCCTGTT151540.16814280754550004No Hit
AACATTCAACGCTGTCGGTGAGTT151160.16772117453199015No Hit
CACTAGATTGTGAGCTCCTGGA150650.16715529864543738No Hit
CCCCCACTGCTAAATTTGACTGG148670.16495836873293843No Hit
CGGGCCGCCGGTGAAATACCACTAC148600.16488069949360765No Hit
CCCCCCACTGCTAAATTTGACTGT147860.16405962467782523No Hit
CCCCCACTGCTAAATTTGACTGA147720.16390428619916367No Hit
ACTGGACTTGGAGTCAGAAGG147420.16357141803060324No Hit
TGAATACCGGGTGCTGTAGGCTTT144810.16067546496412735No Hit
ACTGGACTTGGAGTCAGAAGGC143620.15935508789550423No Hit
GACCCGCGGGCGCTCTCCAGTCCT138610.1537961894805448No Hit
CCCCCACAACCGCGCTTGACTAG131280.14566311056205122No Hit
ATCCCGGACGAGCCCCCA130610.14491970498559956No Hit
TGTCAGTTTGTCAAATACCCCA130060.14430944667657208No Hit
TGTCAGTTTGTCAAATACCCCAA127700.14169088375056324No Hit
CGCCTGTCTGAGCGTCGCT120890.13413477632424112No Hit
AACGGAATCCCAAAAGCAGCTG120660.13387957739501144No Hit
TGAGGTAGTAGGTTGTATAGT119600.1327034431994312No Hit
CAAAGAATTCTCCTTTTGGGC119200.13225961897468394No Hit
CCCCCACTGCTAAATTTGACTGGA113150.1255467775753816No Hit
TGAGAACTGAATTCCATAGGCTGT111480.12369381143706179No Hit
TCGTTTCCCGGGCGGCGCACCA111250.1234386125078321No Hit
GTGCGGTAACGCGACCGATCCCGGA108970.12090881442677272No Hit
TATTGCACTTGTCCCGGCCTGA108120.1199656879491848No Hit
TATTGCACTTGTCCCGGCCTGTAG107780.11958843735814961No Hit
AACATTCAACGCTGTCGGTGAGA106750.11844558997942542No Hit
CAACGGAATCCCAAAAGCAGCTGT105140.11665919747481769No Hit
AACATTCAACGCTGTCGGTGA103050.11434021590051326No Hit
GGAATACCGGGTGCTGTAGGCTTA99820.1107563352856791No Hit
TGAGGTAGTAGGTTGTGTGGTT98100.10884789111926588No Hit
CCCCCACTGCTAAATTTGACTGT97290.10794914706415268No Hit
AAGCTGCCAGTTGAAGAACTG93470.10371062571781633No Hit
TGAATACCGGGTGCTGTAGGCTT93460.10369953011219765No Hit
CCCCCCACTGCTAAATTTGACTGGA92380.10250120470538006No Hit
ACTGGACTTGGAGTCAGAAGGCA92110.10220162335367565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)