FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9013933
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG129686914.387382289173884No Hit
CCCCCACTGCTAAATTTGACTG7865368.725780411281068No Hit
AACATTCAACGCTGTCGGTGAG3886364.311503091935562No Hit
CAACGGAATCCCAAAAGCAGCTG3075573.41201781730572No Hit
AAGCTGCCAGTTGAAGAACTGT2525642.801928969296754No Hit
GCCTGTCTGAGCGTCGCT2519942.795605425511816No Hit
TAGCTTATCAGACTGATGTTGA2386482.647545749452542No Hit
AACATTCAACGCTGTCGGTGAGT2062582.2882131473575407No Hit
TAGCAGCACGTAAATATTGGCG1948832.1620196200703954No Hit
TTCAAGTAATCCAGGATAGGCT1665111.8472624546909768No Hit
CCCATAAAGTAGAAAGCACT1300691.4429772220405899No Hit
CATAAAGTAGAAAGCACTACT1105131.226024200534883No Hit
CAACACCAGTCGATGGGCTGT996721.1057548353199431No Hit
TAGCTTATCAGACTGATGTTGACT856260.9499294037353062No Hit
TAGCTTATCAGACTGATGTTGAC781160.8666139408846283No Hit
TCGTACGACTCTTAGCGG730620.8105451859915088No Hit
TCCTGTCTGAGCGTCGCT704970.7820892389592868No Hit
TATTGCACTTGTCCCGGCCTGT692650.7684215092346481No Hit
TGAGGTAGTAGGTTGTATAGTT650160.721283373195696No Hit
TATTGCACTTGTCCCGGCCTGTAA628210.6969321826554513No Hit
TATTGCACTTGTCCCGGCCTGTA613700.6808348808450206No Hit
CGCCTGTCTGAGCGTCGCT453980.5036425276291714No Hit
TGAGGTAGTAGATTGTATAGTT378750.42018284360445096No Hit
TGTAAACATCCTTGACTGGAAGCT365630.4056275989626282No Hit
TTCACAGTGGCTAAGTTCCG317050.3517332556166104No Hit
TTCTCACTACTGCACTTGACTAG314640.34905961692859266No Hit
ATCCCGGACGAGCCCCCA286660.3180187827000711No Hit
CTCCCACTGCTTCACTTGACTAG280870.3115953934869496No Hit
TAGCTTATCAGACTGATGTTG276670.3069359401717319No Hit
CAACGGAATCCCAAAAGCAGCT271150.3008120872431601No Hit
ACCCCCCACTGCTAAATTTGACTG270840.3004681752127512No Hit
TTCAAGTAATTCAGGATAGGTT267530.29679608224290105No Hit
CATTGCACTTGTCTCGGTCTGA264730.2936897800327559No Hit
TATTGCACTTGTCCCGGCCTGTAT257510.2856799579051675No Hit
TATTGCACTTGTCCCGGCCTG253850.28161957715904923No Hit
CCCATAAAGTAGAAAGCACTA236380.26223847015503665No Hit
AGCAGCATTGTACAGGGCTATGA233580.2591321679448915No Hit
CCCCCCACTGCTAAATTTGACTGG213670.2370441404434668No Hit
CCCCCACTGCTAAATTTGACTGGCTT210040.23301704150674296No Hit
TTCACAGTGGCTAAGTTCTG208610.23143060859227596No Hit
TGAGGTAGTAGTTTGTACAGTT200890.2228660896414473No Hit
TGAGGTAGTAGTTTGTGCTGTT180140.19984617147697903No Hit
TGTAAACATCCCCGACTGGAAGCT176740.19607423307894567No Hit
CCCCCCACTGCTAAATTTGACTGA173080.19201385233282742No Hit
CCCCCCACTGCTAAATTTGACTGTA170130.18874114107571024No Hit
ACCGGGTGCTGTAGGCTT164790.1828169790035049No Hit
TTCAAGTAATCCAGGATAGGC164600.18260619421067362No Hit
TCGTTTCCCGGCCAATGCACCA162040.1797661464756838No Hit
AGCAGCATTGTACAGGGCTATG159730.1772034471523141No Hit
AACATTCAACGCTGTCGGTGAGTT155760.1727991543757869No Hit
TATTGCACTTGTCCCGGCCTGTT150460.1669193680494408No Hit
TGAGGTAGTAGGTTGTATAGT147020.16310305390554822No Hit
TGAGGTAGTAGGTTGTGTGGTT143960.15970830934731822No Hit
CCCCCACTGCTAAATTTGACTGTA143840.15957518210974056No Hit
ACTGGACTTGGAGTCAGAAGG143350.15903157922296515No Hit
AACGGAATCCCAAAAGCAGCTG139760.15504885603210053No Hit
CATAAAGTAGAAAGCACT133460.148059676059274No Hit
ACTGGACTTGGAGTCAGAAGGC131870.1462957401613702No Hit
CCCCCACTGCTAAATTTGACTGA131810.14622917654258136No Hit
CAAAGAATTCTCCTTTTGGGCT131330.14569666759227076No Hit
TAGCAGCACGTAAATATTGGC128940.14304521677718263No Hit
CTTCTCACTACTGCACTTGACTAG128670.1427456804926329No Hit
AACATTCAACGCTGTCGGTGA126980.14087080523008103No Hit
TATTGCACTTGTCCCGGCCTGA126620.14047142351734807No Hit
ACCGGGTGCTGTAGGCTTA126590.14043814170795368No Hit
CCCCCACTGCTAAATTTGACTGG124470.13808622717741523No Hit
CCCATAAAGTAGAAAGCACTAC123800.13734293343427337No Hit
CACTAGATTGTGAGCTCCTGGA122740.13616697616900414No Hit
GACCCGCGGGCGCTCTCCAGTCCT120680.13388162525725453No Hit
AACATTCAACGCTGTCGGTGAGA120100.1332381769422959No Hit
ATCCCACCGCTGCCACCA112560.12487334884783369No Hit
CCCCCCACTGCTAAATTTGACTGGCT110760.12287644028416897No Hit
AAGCTGCCAGTTGAAGAACTG108950.12086843778403944No Hit
CAAAGTGCTGTTCGTGCAGGT108060.11988107743867188No Hit
TACGCCTGTCTGAGCGTCGCT106640.11830573846066972No Hit
CTACGCCTGTCTGAGCGTCGCT105320.11684133884731558No Hit
CAACGGAATCCCAAAAGCAGCTGT102520.11373503663717048No Hit
CACTAGATTGTGAGCTCCTGGAA100430.11141640391602645No Hit
CCCCCACTGCTAAATTTGACTGGCT99430.11030701026954605No Hit
TAGCAGCACATAATGGTTTG98060.1087871409738679No Hit
ACCGGGTGCTGTAGGCTTT96530.1070897686947529No Hit
TGTAAACATCCTACACTCTCAGCT94900.10528145705098985No Hit
TAGCTTATCAGACTGATGTTGACA93130.10331783029671954No Hit
TGAGGTAGTAGATTGTATAGT90840.10077731884627943No Hit
CCCCCACAACCGCGCTTGACTAG90340.10022262202303922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)