FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444193
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9327298.930599041974808No Hit
CCCCCCACTGCTAAATTTGACTG6248585.982827012101366No Hit
AACATTCAACGCTGTCGGTGAGT4742824.541107197080713No Hit
TTCAAGTAATCCAGGATAGGCT3651433.496134167570438No Hit
TAGCAGCACGTAAATATTGGCG3643723.488752074956868No Hit
AAGCTGCCAGTTGAAGAACTGT3606973.453565057635377No Hit
AACATTCAACGCTGTCGGTGAG2768062.650334018147692No Hit
TAGCTTATCAGACTGATGTTGACT2352302.252256349533181No Hit
TATTGCACTTGTCCCGGCCTGTAA2250432.1547188949878655No Hit
TATTGCACTTGTCCCGGCCTGTAT2178712.0860491566940595No Hit
TAGCTTATCAGACTGATGTTGAC2077721.989354275624742No Hit
CCCCCACTGCTAAATTTGACTG1873781.7940878725622937No Hit
TAGCTTATCAGACTGATGTTGA1605601.5373136057520194No Hit
TGAGGTAGTAGGTTGTATAGTT1194501.1436977466808589No Hit
TATTGCACTTGTCCCGGCCTGT1099271.0525178919998892No Hit
TGTAAACATCCTTGACTGGAAGCT1076421.0306397057197239No Hit
CATAAAGTAGAAAGCACTACT871140.8340902930460975No Hit
TGAGGTAGTAGATTGTATAGTT850680.8145004597291527No Hit
TATTGCACTTGTCCCGGCCTGTA836210.8006458708681465No Hit
AGCAGCATTGTACAGGGCTATGA704850.6748726301783202No Hit
TTCAAGTAATTCAGGATAGGTT682690.6536550981009256No Hit
CCCATAAAGTAGAAAGCACTAC654550.6267118962661835No Hit
TCGTACGACTCTTAGCGG653890.6260799661591854No Hit
TGTAAACATCCCCGACTGGAAGCT636470.6094008412138688No Hit
CAACGGAATCCCAAAAGCAGCTGT578860.5542410026317974No Hit
ACTGGACTTGGAGTCAGAAGGC559840.5360299259119398No Hit
CTCCCACTGCTTCACTTGACTAG466530.44668841336041953No Hit
CAACGGAATCCCAAAAGCAGCT450340.43118697634178155No Hit
TTCTCACTACTGCACTTGACTAG410060.3926200904177087No Hit
CTTCTCACTACTGCACTTGACTAG382480.36621307170405604No Hit
CATTGCACTTGTCTCGGTCTGA377820.36175126216070497No Hit
TGTAAACATCCTACACTCTCAGCT366800.351199944313553No Hit
CCCATAAAGTAGAAAGCACT366460.350874404561463No Hit
TAGCTTATCAGACTGATGTTGACA345040.3303654001797937No Hit
CACCCGGCTGTGTGCACATGTGC337490.3231365027436778No Hit
TGAGGTAGTAGTTTGTACAGTT336070.32177689554377253No Hit
AACATTCAACGCTGTCGGTGAGTT313760.30041574298751467No Hit
CCCCCACTGCTAAATTTGACTGGCTT305390.29240172026694644No Hit
TGTCAGTTTGTCAAATACCCCAA289050.2767566627694452No Hit
CAAAGAATTCTCCTTTTGGGCT288860.2765747434962184No Hit
CAACACCAGTCGATGGGCTGT277490.26568831119838554No Hit
CCCCCCACTGCTAAATTTGACTGGCT269740.25826791979045194No Hit
TATTGCACTTGTCCCGGCCTGTAG264930.2536624897682377No Hit
TCGTTTCCCGGCCAATGCACCA256070.24517930681671624No Hit
CTCCCACTGCTTCACTTGACTAGCCT252480.24174199002258961No Hit
AACGGAATCCCAAAAGCAGCTG232810.22290855789432462No Hit
CGCCTGTCTGAGCGTCGCT232170.22229577718450816No Hit
TATTGCACTTGTCCCGGCCTGTT227630.21794886402424773No Hit
TGAGGTAGTAGTTTGTGCTGTT222320.21286469907248937No Hit
CACTAGATTGTGAGCTCCTGGA221000.21160083885849293No Hit
CAAAGAATTCTCCTTTTGGGCTT219290.20996356539945213No Hit
TAGCAGCACGTAAATATTGGC216670.20745499436864104No Hit
CACTAGATTGTGAGCTCCTGGAA213740.2046496076815126No Hit
TTCAAGTAATCCAGGATAGGCTT213670.20458258479137642No Hit
TATTGCACTTGTCCCGGCCTGTAAT200740.19220249951336596No Hit
TATTGCACTTGTCCCGGCCTGTTT198520.19007691642619012No Hit
TGTAAACATCCCCGACTGGAAGC189530.1814692623929872No Hit
CCCCCCACTGCTAAATTTGACTGG188460.1804447696437628No Hit
ACTGGACTTGGAGTCAGAAGGCT172900.16554653863635035No Hit
ACTGGACTTGGAGTCAGAAGGCA171060.16378479409562807No Hit
TGTCAGTTTGTCAAATACCCCA167040.1599357652620935No Hit
TTCACAGTGGCTAAGTTCTGC163850.15688143641160213No Hit
AGCAGCATTGTACAGGGCTATGT161980.1550909677751072No Hit
TTCAAGTAATCCAGGATAGGC155070.1484748510488077No Hit
AGCTCGGTCTGAGGCCCCTCAGT152140.14566946436167927No Hit
TTCACAGTGGCTAAGTTCCGC144880.1387182331846989No Hit
CCCATAAAGTAGAAAGCACTA144310.13817247536501862No Hit
TCGTTTCCCGGGCGGCGCACCA143490.1373873500805663No Hit
TTATAAAGCAATGAGACTGATT140790.13480218146102815No Hit
TATTGCACTTGTCCCGGCCTGTAAA140710.13472558387230107No Hit
ATGCACCTGGGCAAGGATTCTGA138900.13299256342735144No Hit
ACCCCCCACTGCTAAATTTGACTG138200.13232233452598968No Hit
TTCACAGTGGCTAAGTTCCG136500.13069463576553975No Hit
ATCCCGGACGAGCCCCCA135570.12980418879658773No Hit
AACATTCAACGCTGTCGGTGA135530.1297658900022242No Hit
TGATGTAGTAGGTTGTATAGTT132670.12702752620523194No Hit
TGAGAACTGAATTCCATGGGTT132380.12674985994609636No Hit
GGAATACCGGGTGCTGTAGGCTTT127930.12248911907315385No Hit
CAACGGAATCCCAAAAGCAGCTGA127380.12196251065065533No Hit
TAGCAGCACGTAAATATTGGCGT125940.12058375405356832No Hit
CCCCCCACTGCTAAATTTGACTGTA123730.11846774566498341No Hit
AACATTCAACGCTGTCGGTGAGA119620.11453254454413088No Hit
TTCACAGTGGCTAAGTTCTG119300.11422615418922268No Hit
TATTGCACTTGTCCCGGCCTGA118660.11361337347940621No Hit
CCCCCCACTGCTAAATTTGACTGT117040.11206227230768331No Hit
CCTTGGCTTGGCTCTTGACGTGGACA116930.11195695062318362No Hit
TAGCTTATCAGACTGATGTTGACC115560.11064521691623277No Hit
ACCACAGGGTAGAACCACGGAC112190.10741854349110554No Hit
ACTGGACTTGGAGTCAGAAGG111740.10698768205451585No Hit
TGAGGTAGTAGGTTGTGTGGTT111730.10697810735592495No Hit
TAGCAGCACATCATGGTTTACA109200.10455570861243181No Hit
CAAAGTGCTGTTCGTGCAGGT108810.1041822953673874No Hit
TCAGTGCACTACAGAACTTTGT107420.10285141226325481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)