FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11340948
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9786728.629543138721736No Hit
AACATTCAACGCTGTCGGTGAGT4339743.8266113203234866No Hit
AAGCTGCCAGTTGAAGAACTGT4301553.792936886757615No Hit
TAGCAGCACGTAAATATTGGCG4090193.606567987085383No Hit
TTCAAGTAATCCAGGATAGGCT4025163.549227101649703No Hit
CCCCCCACTGCTAAATTTGACTG3925793.461606560580297No Hit
AACATTCAACGCTGTCGGTGAG2837372.5018807951504582No Hit
TAGCTTATCAGACTGATGTTGACT1999101.7627274192598363No Hit
TATTGCACTTGTCCCGGCCTGTAT1996591.7605142004001781No Hit
TATTGCACTTGTCCCGGCCTGTAA1991241.75579678171525No Hit
TAGCTTATCAGACTGATGTTGAC1969351.7364950443296274No Hit
TCGTACGACTCTTAGCGG1858561.6388047983290286No Hit
TAGCTTATCAGACTGATGTTGA1779091.5687312912465519No Hit
CCCCCACTGCTAAATTTGACTG1541041.3588282037797899No Hit
TATTGCACTTGTCCCGGCCTGT1498791.3215738225763842No Hit
TGAGGTAGTAGGTTGTATAGTT1169961.0316245167511569No Hit
CATAAAGTAGAAAGCACTACT1093950.9646019010051011No Hit
TATTGCACTTGTCCCGGCCTGTA960800.8471954901830077No Hit
TGTAAACATCCTTGACTGGAAGCT945630.8338191833698558No Hit
TTCAAGTAATTCAGGATAGGTT915060.8068637648281255No Hit
TGAGGTAGTAGATTGTATAGTT859280.7576791640346116No Hit
AGCAGCATTGTACAGGGCTATGA676160.5962111809347861No Hit
CCCATAAAGTAGAAAGCACTAC602500.5312607023680913No Hit
CAACGGAATCCCAAAAGCAGCTGT601980.5308021869071262No Hit
TGAGAACTGAATTCCATGGGTT581590.5128230902742874No Hit
TGTAAACATCCCCGACTGGAAGCT553780.48830133071767895No Hit
GCCTGTCTGAGCGTCGCT522550.46076395024472383No Hit
CCCATAAAGTAGAAAGCACT516430.4553675759733666No Hit
ACTGGACTTGGAGTCAGAAGGC507760.44772271242227724No Hit
CATTGCACTTGTCTCGGTCTGA503390.44386941902916754No Hit
CAACGGAATCCCAAAAGCAGCT463280.4085020052997333No Hit
CTCCCACTGCTTCACTTGACTAG421350.3715297874569216No Hit
TTCTCACTACTGCACTTGACTAG402540.35494387241701486No Hit
ATCCCGGACGAGCCCCCA402530.35493505481199633No Hit
CGCCTGTCTGAGCGTCGCT391160.3449094379058964No Hit
CAACACCAGTCGATGGGCTGT381080.3360212920471904No Hit
TTAATGCTAATCGTGATAGGGGTT369950.3262072976615359No Hit
TGAGGTAGTAGTTTGTACAGTT359370.316878271551902No Hit
CACCCGGCTGTGTGCACATGTGC328870.28998457624530155No Hit
TACGCCTGTCTGAGCGTCGCT327110.2884326777620354No Hit
TAGCTTATCAGACTGATGTTGACA312290.27536498712453317No Hit
CTTCTCACTACTGCACTTGACTAG299110.26374338371007433No Hit
TGTAAACATCCTACACTCTCAGCT278580.2456408406069757No Hit
TCGTTTCCCGGCCAATGCACCA271420.2393274354136885No Hit
AACATTCAACGCTGTCGGTGAGTT268340.2366116130679728No Hit
TATTGCACTTGTCCCGGCCTGTT267820.2361530976070078No Hit
TAGCAGCACGTAAATATTGGC256660.2263126504062976No Hit
CGTTCGCGCTTTCCCCTG256630.2262861975912419No Hit
TATTGCACTTGTCCCGGCCTGTAG251500.22176276621672192No Hit
CAAAGAATTCTCCTTTTGGGCT250530.22090745852992186No Hit
CTACGCCTGTCTGAGCGTCGCT236920.20890669809966503No Hit
TGAGGTAGTAGTTTGTGCTGTT235410.20757523974186284No Hit
TTCAAGTAATCCAGGATAGGCTT225390.1987399995132682No Hit
AACGGAATCCCAAAAGCAGCTG224200.19769070451605988No Hit
CCCCCACTGCTAAATTTGACTGGCTT215040.18961377831906115No Hit
CACTAGATTGTGAGCTCCTGGA200940.17718095524289504No Hit
AGCTCGGTCTGAGGCCCCTCAGT200060.17640500600126197No Hit
TTCAAGTAATCCAGGATAGGC192740.16995051912767786No Hit
CAAAGAATTCTCCTTTTGGGCTT191960.16926274593623036No Hit
TTCACAGTGGCTAAGTTCCG191940.16924511072619325No Hit
TGTAAACATCCCCGACTGGAAGC190650.16810763967879935No Hit
TCCTGTCTGAGCGTCGCT188220.16596496165928984No Hit
CTCCCACTGCTTCACTTGACTAGCCT186460.16441306317602375No Hit
TTCACAGTGGCTAAGTTCCGC184030.16227038515651424No Hit
TATTGCACTTGTCCCGGCCTGTTT183970.1622174795264029No Hit
TGATGTAGTAGGTTGTATAGTT183380.161697240830308No Hit
TTCACAGTGGCTAAGTTCTGC181050.1596427388609841No Hit
TCGTTTCCCGGGCGGCGCACCA180980.15958101562585422No Hit
CCCCCCACTGCTAAATTTGACTGGCT172420.15203314572996895No Hit
CACTAGATTGTGAGCTCCTGGAA170510.15034898317142448No Hit
TTATAAAGCAATGAGACTGATT168430.1485149213275645No Hit
AGCAGCATTGTACAGGGCTATGT167340.14755380238054172No Hit
CCCATAAAGTAGAAAGCACTA159540.14067607046606687No Hit
ACTGGACTTGGAGTCAGAAGGCT151840.13388651460177756No Hit
TATTGCACTTGTCCCGGCCTGTAAT149630.13193782389267636No Hit
TTCACAGTGGCTAAGTTCTG149060.13143522040661856No Hit
TGTCAGTTTGTCAAATACCCCAA148440.13088852889546798No Hit
TATTGCACTTGTCCCGGCCTGA148140.13062400074491126No Hit
ACTGGACTTGGAGTCAGAAGGCA143580.12660317285644904No Hit
ACCACAGGGTAGAACCACGGAC140520.12390498572077042No Hit
TGAGAACTGAATTCCATAGGCTGT140030.12347292307486112No Hit
CAAAGTGCTGTTCGTGCAGGT137190.12096872324959078No Hit
ATCCCACCGCTGCCACCA137080.12087172959438663No Hit
GGAATACCGGGTGCTGTAGGCTTT134470.11857033468454313No Hit
TGATGTAGTAGATTGTATAGTT134280.11840280018919054No Hit
CCCCCCACTGCTAAATTTGACTGG130380.1149639342319531No Hit
TGTCAGTTTGTCAAATACCCCA130200.11480521734161905No Hit
TAGCAGCACGTAAATATTGGCGT127950.11282125621244361No Hit
TAGTGGGTTATCAGAACT125480.11064330777285991No Hit
AACATTCAACGCTGTCGGTGA123190.10862407622361024No Hit
AACCGGGCGGAAACACCA122480.10799802626729266No Hit
TGAGGTAGTAGGTTGTGTGGTT120620.10635795173384094No Hit
CAAAGTGCTGTTCGTGCAGGTAG119010.10493831732585318No Hit
AGCAGCATTGTACAGGGCTATG118740.10470024199035212No Hit
TGAGAACTGAATTCCATGGGTTG117020.10318361392716022No Hit
ACTGGACTTGGAGTCAGAAGG115380.10173752670411682No Hit
ACTTGACTTGGAGTCAGAAGGC113490.10007099935560942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)