FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10288667
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG107989410.49595637607865No Hit
CCCCCCACTGCTAAATTTGACTG5085314.942632510120116No Hit
AACATTCAACGCTGTCGGTGAGT4374674.251930789479337No Hit
AACATTCAACGCTGTCGGTGAG3186183.0967860073613034No Hit
TTCAAGTAATCCAGGATAGGCT2871222.7906627748764734No Hit
TAGCAGCACGTAAATATTGGCG2807682.728905503502057No Hit
TATTGCACTTGTCCCGGCCTGTAA2800142.721577051721083No Hit
TATTGCACTTGTCCCGGCCTGTAT2560062.488232926578341No Hit
AAGCTGCCAGTTGAAGAACTGT2540692.4694063866582523No Hit
TAGCTTATCAGACTGATGTTGACT2170592.1096902057380222No Hit
TAGCTTATCAGACTGATGTTGAC1635521.5896325539547542No Hit
CCCCCACTGCTAAATTTGACTG1611401.5661892838012934No Hit
TAGCTTATCAGACTGATGTTGA1116731.0853981375818655No Hit
TGAGGTAGTAGGTTGTATAGTT878930.8542700429511423No Hit
TATTGCACTTGTCCCGGCCTGT868270.8439091283642478No Hit
GGAATACCGGGTGCTGTAGGCTTT831760.8084234818757376No Hit
TGTAAACATCCTTGACTGGAAGCT827880.8046523422324777No Hit
TATTGCACTTGTCCCGGCCTGTA800460.7780016594958317No Hit
TCGTACGACTCTTAGCGG686590.6673264865118095No Hit
CAACGGAATCCCAAAAGCAGCTGT657240.6387999533856038No Hit
TGTAAACATCCCCGACTGGAAGCT621810.6043640055606815No Hit
TGAGGTAGTAGATTGTATAGTT588880.5723579157533235No Hit
TTAATGCTAATCGTGATAGGGGTT580990.5646892838498904No Hit
AGCAGCATTGTACAGGGCTATGA549390.5339758785078766No Hit
TTCAAGTAATTCAGGATAGGTT512680.4982958433779614No Hit
CCCATAAAGTAGAAAGCACTAC482210.4686807338598868No Hit
GCCTGTCTGAGCGTCGCT479160.4657163070784583No Hit
TGAGAACTGAATTCCATGGGTT466350.4532657145964584No Hit
ACTGGACTTGGAGTCAGAAGGC464920.45187583581041163No Hit
CATAAAGTAGAAAGCACTACT459160.4462774429379433No Hit
GGAATACCGGGTGCTGTAGGCTT449120.43651913313940477No Hit
CAACGGAATCCCAAAAGCAGCT433260.42110411387597635No Hit
TAGCTTATCAGACTGATGTTGACA355650.3456716015787079No Hit
TATTGCACTTGTCCCGGCCTGTAG340550.3309952591526191No Hit
TACGCCTGTCTGAGCGTCGCT336030.3266020758568627No Hit
CACCCGGCTGTGTGCACATGTGC331890.3225782309797761No Hit
CAAAGAATTCTCCTTTTGGGCT314290.3054720305361229No Hit
CCCCCACTGCTAAATTTGACTGGCTT312260.3034989858258606No Hit
CCCATAAAGTAGAAAGCACT293790.285547194792095No Hit
TCGTTTCCCGGCCAATGCACCA287620.2795503052047461No Hit
CATTGCACTTGTCTCGGTCTGA286210.27817986528283983No Hit
CTCCCACTGCTTCACTTGACTAG279760.27191083159752377No Hit
TTCTCACTACTGCACTTGACTAG275450.2677217563752428No Hit
AACATTCAACGCTGTCGGTGAGTT272070.2644365883354957No Hit
TATTGCACTTGTCCCGGCCTGTAAT268080.260558534939463No Hit
CTTCTCACTACTGCACTTGACTAG260810.2534925078243858No Hit
TGTAAACATCCTACACTCTCAGCT256510.24931315203417506No Hit
TGAATACCGGGTGCTGTAGGCTTT252460.24537678204572078No Hit
CACTAGATTGTGAGCTCCTGGAA251920.24485193271392688No Hit
TATTGCACTTGTCCCGGCCTGTAAA225190.2188718907901286No Hit
TGAGGTAGTAGTTTGTACAGTT224320.21802630020001618No Hit
TATTGCACTTGTCCCGGCCTGTTT221810.21558672275038157No Hit
TAGCAGCACGTAAATATTGGC216190.21012440192689683No Hit
TGAGAACTGAATTCCATAGGCTGT215490.20944404168197883No Hit
AACGGAATCCCAAAAGCAGCTG212560.20659624808539337No Hit
CACTAGATTGTGAGCTCCTGGA210610.20470095883169315No Hit
CTACGCCTGTCTGAGCGTCGCT209250.20337911607013814No Hit
CAAAGAATTCTCCTTTTGGGCTT206960.2011533661260492No Hit
CTCCCACTGCTTCACTTGACTAGCCT206820.20101729407706556No Hit
CCCCCCACTGCTAAATTTGACTGGCT202750.19706148522447078No Hit
TATTGCACTTGTCCCGGCCTGTT200160.19454415231827407No Hit
TGTCAGTTTGTCAAATACCCCAA191490.18611740471336083No Hit
ACTGGACTTGGAGTCAGAAGGCT186740.18150067447998852No Hit
ACTGGACTTGGAGTCAGAAGGCA176360.17141190399106124No Hit
TGTAAACATCCCCGACTGGAAGC171010.16621200783347348No Hit
CAACACCAGTCGATGGGCTGT168730.16399597732145477No Hit
TTCAAGTAATCCAGGATAGGCTT166100.16143976668697704No Hit
ATGCACCTGGGCAAGGATTCTGA161090.15657033121977804No Hit
TCGTTTCCCGGGCGGCGCACCA161020.15650229519528625No Hit
CGTTCGCGCTTTCCCCTG152220.14794919497345965No Hit
TCCTGTCTGAGCGTCGCT146600.1424868741499749No Hit
TGAGGTAGTAGTTTGTGCTGTT139670.13575130772528649No Hit
TGAATACCGGGTGCTGTAGGCTT136370.13254389514210152No Hit
GGAATACCGGGTGCTGTAGGCTTA134580.1308041168015254No Hit
AACATTCAACGCTGTCGGTGA131170.1274897904655676No Hit
AACCGGGCGGAAACACCA131040.12736343784865425No Hit
CCCCCCACTGCTAAATTTGACTGG127300.12372837025437795No Hit
AGCTCGGTCTGAGGCCCCTCAGT125250.12173588667997515No Hit
CCTTGGCTTGGCTCTTGACGTGGACA122890.1194421007113944No Hit
AACATTCAACGCTGTCGGTGAGA121720.11830492715917426No Hit
TGTCAGTTTGTCAAATACCCCA119610.11625412699234994No Hit
AGCAGCATTGTACAGGGCTATGT119170.1158264719812586No Hit
CCCATAAAGTAGAAAGCACTA118880.11554460845122114No Hit
ACCCCCCACTGCTAAATTTGACTG118720.11538909753809701No Hit
TTCAAGTAATCCAGGATAGGC117660.11435883773864972No Hit
TTCACAGTGGCTAAGTTCTGC115970.11271625371877621No Hit
CCCCCCACTGCTAAATTTGACTGTA110220.10712758027837815No Hit
TAGCAGCACATCATGGTTTACA105810.10284131073539457No Hit
TAGCTTATCAGACTGATGTTGACC104900.10195684241700116No Hit
ATCACATTGCCAGGGATTTCC104680.10174301491145549No Hit
CCCCCCACTGCTAAATTTGACTGT104400.10147087081348828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)