FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10071425
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7609037.555067927329053No Hit
GGAATACCGGGTGCTGTAGGCTTT4906314.871515202664965No Hit
CCCCCCACTGCTAAATTTGACTG3334413.3107628761570482No Hit
GGAATACCGGGTGCTGTAGGCTT2950282.929357067147896No Hit
AACATTCAACGCTGTCGGTGAGT2873992.8536081041163492No Hit
TTCAAGTAATCCAGGATAGGCT2651682.6328746925087563No Hit
AAGCTGCCAGTTGAAGAACTGT2393892.3769128996145037No Hit
GCCTGTCTGAGCGTCGCT2343582.326959690411238No Hit
AACATTCAACGCTGTCGGTGAG2206762.1911099968475165No Hit
TAGCAGCACGTAAATATTGGCG2199022.1834248877393216No Hit
TGAATACCGGGTGCTGTAGGCTTT1909671.8961269135201821No Hit
TAGCTTATCAGACTGATGTTGAC1653121.641396326736286No Hit
TAGCTTATCAGACTGATGTTGACT1577101.5659154489061875No Hit
TATTGCACTTGTCCCGGCCTGTAA1547641.536664374703679No Hit
TATTGCACTTGTCCCGGCCTGTAT1417011.4069607826102066No Hit
TCGTACGACTCTTAGCGG1416101.4060572361905093No Hit
CCCCCACTGCTAAATTTGACTG1297371.2881692511238478No Hit
TGAATACCGGGTGCTGTAGGCTT1147801.1396599786028294No Hit
TAGCTTATCAGACTGATGTTGA1127371.1193748650265478No Hit
TGAGGTAGTAGGTTGTATAGTT1005490.9983592192763189No Hit
TATTGCACTTGTCCCGGCCTGT990490.9834655969736159No Hit
AACCGGGCGGAAACACCA957500.9507095569892047No Hit
TCCTGTCTGAGCGTCGCT891740.885415916814155No Hit
TGTAAACATCCTTGACTGGAAGCT837700.8317591601982838No Hit
GGAATACCGGGTGCTGTAGGCTTA764080.7586612619366176No Hit
TATTGCACTTGTCCCGGCCTGTA743910.7386343044802498No Hit
TGAGGTAGTAGATTGTATAGTT658670.6539988134747565No Hit
AGCAGCATTGTACAGGGCTATGA646760.6421732773664104No Hit
TTCAAGTAATTCAGGATAGGTT616780.6124058909240748No Hit
TTAATGCTAATCGTGATAGGGGTT593410.5892016273764635No Hit
CATAAAGTAGAAAGCACTACT591810.587612974330842No Hit
TGTAAACATCCCCGACTGGAAGCT484360.4809249932358132No Hit
GGAATACCGGGTGCTGTAGGCT464820.46152356791615884No Hit
CAACGGAATCCCAAAAGCAGCTGT428210.4251732004160285No Hit
CCCATAAAGTAGAAAGCACTAC408820.4059207113194012No Hit
ACTGGACTTGGAGTCAGAAGGC407580.4046895052090444No Hit
CATTGCACTTGTCTCGGTCTGA362530.3599589928932599No Hit
CCCATAAAGTAGAAAGCACT351720.3492256557537786No Hit
CAACGGAATCCCAAAAGCAGCT338330.3359306155782325No Hit
TGAGAACTGAATTCCATGGGTT325940.32362848355619983No Hit
TAGCTTATCAGACTGATGTTGACA319380.3171150060691511No Hit
TGAATACCGGGTGCTGTAGGCTTA309580.3073845061647185No Hit
CGCCTGTCTGAGCGTCGCT283970.28195612835323697No Hit
TGAGGTAGTAGTTTGTACAGTT276230.2742710192450423No Hit
TGTAAACATCCTACACTCTCAGCT269150.2672412295181665No Hit
CAACACCAGTCGATGGGCTGT252560.25076888325137703No Hit
CTCCCACTGCTTCACTTGACTAG228790.22716745644236042No Hit
CAAAGAATTCTCCTTTTGGGCT226420.2248142641185334No Hit
TTCTCACTACTGCACTTGACTAG221540.21996887232938736No Hit
TCGTTTCCCGGCCAATGCACCA221160.2195915672310522No Hit
AACATTCAACGCTGTCGGTGAGTT206770.2053036189019925No Hit
CACCCGGCTGTGTGCACATGTGC202730.20129226996179783No Hit
AACGGAATCCCAAAAGCAGCTG202700.20126248271719244No Hit
TACGCCTGTCTGAGCGTCGCT200080.19866106335498698No Hit
TATTGCACTTGTCCCGGCCTGTT197360.19596035317743019No Hit
TGAGGTAGTAGTTTGTGCTGTT195400.1940142531965437No Hit
TATTGCACTTGTCCCGGCCTGTAG191360.19000290425634903No Hit
TGAATACCGGGTGCTGTAGGCT182070.18077878751020834No Hit
CCCCCACTGCTAAATTTGACTGGCTT180740.17945821966603534No Hit
AGGTCCCTGTTCGGGCGCCA171630.17041282638752708No Hit
TAGCAGCACGTAAATATTGGC171140.16992630139230547No Hit
CTTCTCACTACTGCACTTGACTAG170430.16922133660331085No Hit
ATCCCGGACGAGCCCCCA169040.16784119426992705No Hit
CTACGCCTGTCTGAGCGTCGCT168470.16727523662242436No Hit
TGTCAGTTTGTCAAATACCCCAA156540.15542984235100793No Hit
TTCAAGTAATCCAGGATAGGCTT153300.1522128199336241No Hit
CGAATACCGGGTGCTGTAGGCTTT151990.15091211025252135No Hit
AGCAGCATTGTACAGGGCTATGT148100.1470496975353537No Hit
AGCTCGGTCTGAGGCCCCTCAGT143040.14202558227857529No Hit
CACTAGATTGTGAGCTCCTGGAA139380.13839153843671576No Hit
TGAGAACTGAATTCCATAGGCTGT138320.13733905579399142No Hit
TCGTTTCCCGGGCGGCGCACCA138190.13720997773403465No Hit
GGAATACCGGGTGCTGTAGGCTA137290.13631636039587247No Hit
TTCACAGTGGCTAAGTTCCG136160.13519437418240218No Hit
CAAAGAATTCTCCTTTTGGGCTT134450.13349650123989407No Hit
CACTAGATTGTGAGCTCCTGGA134300.133347565016867No Hit
TGTCAGTTTGTCAAATACCCCA131760.13082557830694266No Hit
TTCAAGTAATCCAGGATAGGC129890.12896884005987236No Hit
ACTGGACTTGGAGTCAGAAGGCT129560.1286411803692129No Hit
CTCCCACTGCTTCACTTGACTAGCCT126840.12594047019165608No Hit
CCCCCCACTGCTAAATTTGACTGGCT124360.12347805797094254No Hit
TATTGCACTTGTCCCGGCCTGTTT123850.12297167481265063No Hit
TCCTTCTGATCGAGGCCC123670.1227929513450182No Hit
TATTGCACTTGTCCCGGCCTGTAAT122660.12179011410996955No Hit
TGTAAACATCCCCGACTGGAAGC121480.12061848248882358No Hit
TGAGGTAGTAGGTTGTGTGGTT120010.11915890750315869No Hit
ACTGGACTTGGAGTCAGAAGGCA119010.11816599934964517No Hit
TTCACAGTGGCTAAGTTCTG115030.11421422489866132No Hit
GGAATACCGGGTGCTGTAGGCGTT113540.11273479174992616No Hit
CCCATAAAGTAGAAAGCACTA111990.11119578411198018No Hit
TTATAAAGCAATGAGACTGATT111630.11083833717671532No Hit
TTAATGCTAATCGTGATAGGGGTTT110300.10951776933254233No Hit
TATTGCACTTGTCCCGGCCTGTAAA108590.10781989639003417No Hit
TTCACAGTGGCTAAGTTCTGC108320.10755181118858553No Hit
GAATACCGGGTGCTGTAGGCTTT107730.10696599537801256No Hit
ACCGGGTGCTGTAGGCTT105630.10488088825563414No Hit
AGCAGCATTGTACAGGGCTATG103830.10309365357930977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)