FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8412112
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG84926310.09571674747079No Hit
TAGCAGCACGTAAATATTGGCG3474154.129937880047247No Hit
CCCCCCACTGCTAAATTTGACTG3268043.8849221218167327No Hit
TTCAAGTAATCCAGGATAGGCT3237783.848950180406538No Hit
AACATTCAACGCTGTCGGTGAGT3234273.8447776254048924No Hit
AAGCTGCCAGTTGAAGAACTGT2797613.325692763006484No Hit
AACATTCAACGCTGTCGGTGAG2300202.734390602502677No Hit
TATTGCACTTGTCCCGGCCTGTAA1572071.8688172482724907No Hit
TATTGCACTTGTCCCGGCCTGTAT1503511.7873157180979045No Hit
TAGCTTATCAGACTGATGTTGAC1418651.6864373655510054No Hit
TAGCTTATCAGACTGATGTTGACT1299421.5447012593270275No Hit
CCCCCACTGCTAAATTTGACTG1178391.400825381307334No Hit
TAGCTTATCAGACTGATGTTGA1157731.3762655561409547No Hit
TGAGGTAGTAGGTTGTATAGTT1013161.2044062180817374No Hit
TATTGCACTTGTCCCGGCCTGT1000461.1893089393008558No Hit
TATTGCACTTGTCCCGGCCTGTA744570.8851166032977211No Hit
TGTAAACATCCTTGACTGGAAGCT672560.7995138438480135No Hit
CAACGGAATCCCAAAAGCAGCT668440.7946161439600424No Hit
TGAGGTAGTAGATTGTATAGTT642160.7633754757425959No Hit
CATAAAGTAGAAAGCACTACT619820.7368185302335489No Hit
AGCAGCATTGTACAGGGCTATGA573690.6819809341577953No Hit
TTCAAGTAATTCAGGATAGGTT527060.6265489570276763No Hit
CAACGGAATCCCAAAAGCAGCTGT447140.5315430892979076No Hit
GCCTGTCTGAGCGTCGCT437430.5200002092221312No Hit
TACGCCTGTCTGAGCGTCGCT408350.48543100710023834No Hit
ACTGGACTTGGAGTCAGAAGGC387550.46070475523863685No Hit
CTCCCACTGCTTCACTTGACTAG387150.4602292503951445No Hit
CATTGCACTTGTCTCGGTCTGA381610.45364350831277567No Hit
CCCATAAAGTAGAAAGCACTAC369410.4391406105862594No Hit
TGTAAACATCCCCGACTGGAAGCT352380.4188959918745732No Hit
TCGTTTCCCGGCCAATGCACCA339910.4040721283786996No Hit
TTCTCACTACTGCACTTGACTAG330260.39260057402944704No Hit
CTACGCCTGTCTGAGCGTCGCT287380.34162645480706866No Hit
CCCATAAAGTAGAAAGCACT282400.3357064195055891No Hit
TGAGGTAGTAGTTTGTACAGTT276320.32847874588450554No Hit
CTTCTCACTACTGCACTTGACTAG268390.31905186236227No Hit
CAACACCAGTCGATGGGCTGT248410.29530039542982783No Hit
TAGCTTATCAGACTGATGTTGACA246140.29260190544300885No Hit
CACCCGGCTGTGTGCACATGTGC245550.29190053579885766No Hit
CGCCTGTCTGAGCGTCGCT245260.2915557947873257No Hit
CGTTCGCGCTTTCCCCTG222760.26480864734088183No Hit
TGTAAACATCCTACACTCTCAGCT222700.264737321614358No Hit
AACGGAATCCCAAAAGCAGCTG222040.2639527386225956No Hit
TTCACAGTGGCTAAGTTCTGC203840.24231726824369432No Hit
AACATTCAACGCTGTCGGTGAGTT195990.23298548569015723No Hit
TATTGCACTTGTCCCGGCCTGTT194660.23140443208554523No Hit
TAGCAGCACGTAAATATTGGC191910.2281353362865354No Hit
TTCACAGTGGCTAAGTTCCG191230.22732697805259847No Hit
TATTGCACTTGTCCCGGCCTGTAG188210.22373691648423127No Hit
TCCTGTCTGAGCGTCGCT185640.22068179786479303No Hit
TGAGGTAGTAGTTTGTGCTGTT182850.217365151581434No Hit
TTCACAGTGGCTAAGTTCCGC180950.21510650357484543No Hit
CAACGGAATCCCAAAAGCAGCTGA179890.2138464157395907No Hit
CAAAGAATTCTCCTTTTGGGCT171860.2043006560064821No Hit
ATCCCGGACGAGCCCCCA166540.19797644158803404No Hit
CCCCCACTGCTAAATTTGACTGGCTT166330.19772680154520056No Hit
AGCTCGGTCTGAGGCCCCTCAGT152240.180977143433183No Hit
TATTGCACTTGTCCCGGCCTGA147230.17502144526844152No Hit
TTCAAGTAATCCAGGATAGGC143620.17073001405592317No Hit
TTCAAGTAATCCAGGATAGGCTT140810.1673895925303895No Hit
TATTGCACTTGTCCCGGCCTGTTT140740.16730637918277835No Hit
TCGTACGACTCTTAGCGG140030.16646235808557946No Hit
CTCCCACTGCTTCACTTGACTAGCCT138960.16519038262923746No Hit
AGCAGCATTGTACAGGGCTATGT136290.1620163877989261No Hit
CACTAGATTGTGAGCTCCTGGA130800.1554900838219938No Hit
TAGCAGCACATCATGGTTTACA127910.15205456132776168No Hit
ACTGGACTTGGAGTCAGAAGGCT124860.14842883689613262No Hit
ACTGGACTTGGAGTCAGAAGGCA124200.14764425390437028No Hit
TGTAAACATCCCCGACTGGAAGC122440.145552032593004No Hit
TGAGAACTGAATTCCATGGGTT122020.14505275250733704No Hit
AATGCACCCGGGCAAGGATTCT119020.14148646618114452No Hit
TTCACAGTGGCTAAGTTCTG117400.13956067156500057No Hit
TGAGGTAGTAGGTTGTGTGGTT116030.1379320674760393No Hit
CACTAGATTGTGAGCTCCTGGAA112580.1338308382009179No Hit
TGATGTAGTAGGTTGTATAGTT105460.12536685198675435No Hit
CAAAGAATTCTCCTTTTGGGCTT104670.12442772992085699No Hit
CCCCCCACTGCTAAATTTGACTGGCT103820.12341728212843575No Hit
CCCATAAAGTAGAAAGCACTA103260.1227515753475465No Hit
ACTGGACTTGGAGTCAGAAGG101750.12095654456336292No Hit
CAAAGTGCTGTTCGTGCAGGT101050.12012441108725135No Hit
ATCCCACCGCTGCCACCA100890.11993420914985439No Hit
CCCCCCACTGCTAAATTTGACTGG100550.11953003003288591No Hit
TAGCAGCACGTAAATATTGGCGT100330.11926850236896513No Hit
TATTGCACTTGTCCCGGCCTGTAAT100290.1192209518846159No Hit
ATCACATTGCCAGGGATTTCC100120.11901886232613165No Hit
AACATTCAACGCTGTCGGTGAGA97290.11565466555842338No Hit
AATGACACGATCACTCCCGTTG94350.11215970495875471No Hit
AGCAGCATTGTACAGGGCTATG93110.11068563994392847No Hit
TGTCAGTTTGTCAAATACCCCAA92720.11022202272152344No Hit
TGAGGTAGTAGGTTGTATAGT91960.109318563518888No Hit
TATTGCACTTGTCCCGGCCTGTAAA89370.10623966965727513No Hit
CAAAGTGCTGTTCGTGCAGGTAG89060.10587115340356856No Hit
TCAGGCTCAGTCCCCTCCCGAT88090.10471805415809966No Hit
ACCACAGGGTAGAACCACGGAC88000.10461106556831387No Hit
ATGCACCTGGGCAAGGATTCTGA85790.10198390130801871No Hit
TGTAAACATCCTACACTCAGCT85360.10147273360126445No Hit
TCCCACTGCTTCACTTGACTAG84640.10061682488297825No Hit
TCGTTTCCCGGGCGGCGCACCA84400.10033152197688286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)