FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394419
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89574410.670708717303723No Hit
CAACGGAATCCCAAAAGCAGCTG6828328.13435688640274No Hit
AACATTCAACGCTGTCGGTGAGT2769323.299001396046588No Hit
GGAATACCGGGTGCTGTAGGCTTT2703843.2209971887274156No Hit
CCCCCACTGCTAAATTTGACTG2492972.9697945742284246No Hit
AAGCTGCCAGTTGAAGAACTGT2345042.7935703471556517No Hit
TATTGCACTTGTCCCGGCCTGTAT1856132.2111476684687768No Hit
TATTGCACTTGTCCCGGCCTGTAA1833682.1844037091786817No Hit
AACATTCAACGCTGTCGGTGAG1830042.180067494843896No Hit
TAGCAGCACGTAAATATTGGCG1768462.1067092314548512No Hit
GGAATACCGGGTGCTGTAGGCTT1681852.003533538175781No Hit
TAGCTTATCAGACTGATGTTGACT1621011.9310568128657861No Hit
TTCAAGTAATCCAGGATAGGCT1508211.7966818191943956No Hit
TAGCTTATCAGACTGATGTTGAC1429671.7031196560476671No Hit
TATTGCACTTGTCCCGGCCTGT951981.1340630006674672No Hit
TAGCTTATCAGACTGATGTTGA926931.1042217454239536No Hit
TGTAAACATCCTTGACTGGAAGCT809320.9641167542387389No Hit
TATTGCACTTGTCCCGGCCTGTA735110.8757127801221265No Hit
TGTAAACATCCCCGACTGGAAGCT638640.7607911875735534No Hit
TGAGGTAGTAGGTTGTATAGTT614610.7321650253579193No Hit
ACTGGACTTGGAGTCAGAAGGC609990.7266613687022294No Hit
TGAGGTAGTAGATTGTATAGTT499180.5946569977028786No Hit
GGAATACCGGGTGCTGTAGGCTTA414720.49404252992375053No Hit
CAACGGAATCCCAAAAGCAGCTGT403700.48091476015195334No Hit
CATAAAGTAGAAAGCACTACT397640.47369567804513923No Hit
AGCAGCATTGTACAGGGCTATGA365200.43505095468787064No Hit
CCCATAAAGTAGAAAGCACTAC350900.4180158269440684No Hit
GCCTGTCTGAGCGTCGCT316560.3771076950054554No Hit
CATTGCACTTGTCTCGGTCTGA301920.3596675362523601No Hit
TATTGCACTTGTCCCGGCCTGTAG292620.3485887468805167No Hit
TGAATACCGGGTGCTGTAGGCTTT288330.34347820855737604No Hit
TTCAAGTAATTCAGGATAGGTT282580.3366284194296234No Hit
AGCTCGGTCTGAGGCCCCTCAGT278960.33231603044832525No Hit
CACTAGATTGTGAGCTCCTGGAA274130.32656220758101306No Hit
CACTAGATTGTGAGCTCCTGGA266560.3175443112858674No Hit
CAAAGAATTCTCCTTTTGGGCT263060.3133748744254963No Hit
CACCCGGCTGTGTGCACATGTGC261060.31099233907671275No Hit
ATCCCGGACGAGCCCCCA254560.30324909919316634No Hit
CGCCTGTCTGAGCGTCGCT243470.2900379406841617No Hit
GGAATACCGGGTGCTGTAGGCT235640.28071031479367425No Hit
CCCATAAAGTAGAAAGCACT230460.2745395482403249No Hit
TAGCTTATCAGACTGATGTTGACA223370.2660934604288874No Hit
CCCCCACTGCTAAATTTGACTGGCTT219160.261078223519698No Hit
TCGTACGACTCTTAGCGG215580.2568134852453755No Hit
TTCTCACTACTGCACTTGACTAG213400.2542165217152015No Hit
CTCCCACTGCTTCACTTGACTAG199050.23712183058767974No Hit
AACATTCAACGCTGTCGGTGAGTT197920.23577569811561705No Hit
TATTGCACTTGTCCCGGCCTGTT197200.23491798539005498No Hit
TGAGGTAGTAGTTTGTACAGTT194110.23123696827618442No Hit
CAACACCAGTCGATGGGCTGT190550.22699605535534978No Hit
CTTCTCACTACTGCACTTGACTAG183690.2188239591090223No Hit
CCCCCCACTGCTAAATTTGACTGGCT179570.21391593629052824No Hit
TGAATACCGGGTGCTGTAGGCTT176800.2106161248324631No Hit
CAAAGAATTCTCCTTTTGGGCTT175060.2085433190790214No Hit
ACTGGACTTGGAGTCAGAAGGCT174990.20845993034181398No Hit
TGTAAACATCCTACACTCTCAGCT173520.20670876686045814No Hit
TCGTTTCCCGGGCGGCGCACCA172550.20555323721629812No Hit
TGTAAACATCCCCGACTGGAAGC172380.20535072171165153No Hit
TATTGCACTTGTCCCGGCCTGTTT171710.20455257236980906No Hit
TATTGCACTTGTCCCGGCCTGTAAT170210.2027656708582214No Hit
ACTGGACTTGGAGTCAGAAGGCA166320.19813163960483746No Hit
CCCCCCACTGCTAAATTTGACTGG166240.19803633819088612No Hit
ACCCCCCACTGCTAAATTTGACTG165450.19709523672811663No Hit
AACGGAATCCCAAAAGCAGCTG160460.19115081103290174No Hit
TGTCAGTTTGTCAAATACCCCAA160200.19084108143755987No Hit
CCCCCCACTGCTAAATTTGACTGT158020.18824411790738585No Hit
TGAGGTAGTAGTTTGTGCTGTT154500.18405085569352686No Hit
TATTGCACTTGTCCCGGCCTGTAAA133600.15915336129873908No Hit
ACTGGACTTGGAGTCAGAAGG122690.14615663097112497No Hit
CCCCCCACTGCTAAATTTGACTGTA116740.139068588308494No Hit
ACCGGGTGCTGTAGGCTTA113930.13572112614345316No Hit
ACCGGGTGCTGTAGGCTT110190.131265785041228No Hit
TGAGGGGCAGAGAGCGAGACTTT109550.13050337372961726No Hit
TAGCAGCACGTAAATATTGGC108160.12884751166221273No Hit
CAACGGAATCCCAAAAGCAGCT100660.11991300410427452No Hit
TGTCAGTTTGTCAAATACCCCA100360.119555623801957No Hit
CCCCCCACTGCTAAATTTGACTGA97020.1155767897694885No Hit
TTATAAAGCAATGAGACTGATT94910.1130632149765219No Hit
TTCAAGTAATCCAGGATAGGCTT93770.11170516982771529No Hit
GAATACCGGGTGCTGTAGGCTTT92860.1106211162440188No Hit
ACCACAGGGTAGAACCACGGAC91970.10956088801381014No Hit
TACGCCTGTCTGAGCGTCGCT91340.10881038937894333No Hit
ACCGGGTGCTGTAGGCTTT90230.10748808226036846No Hit
CTACGCCTGTCTGAGCGTCGCT89410.10651124276736723No Hit
TTCACAGTGGCTAAGTTCCG89300.10638020332318412No Hit
AACCGGGCGGAAACACCA89150.10620151317202538No Hit
GACCCGCGGGCGCTCTCCAGTCCT87920.1047362539325235No Hit
TTCACAGTGGCTAAGTTCTGC85040.10130540303027523No Hit
ATGCACCTGGGCAAGGATTCTGA84770.10098376075818945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)