FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11149268
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9907158.885919685489666No Hit
CCCCCCACTGCTAAATTTGACTG8493857.618302833872143No Hit
TAGCAGCACGTAAATATTGGCG3744823.358803465841883No Hit
AACATTCAACGCTGTCGGTGAGT3740483.35491083360809No Hit
AAGCTGCCAGTTGAAGAACTGT3425953.072802627042421No Hit
TTCAAGTAATCCAGGATAGGCT3251932.916720631345484No Hit
CCCCCACTGCTAAATTTGACTG2908472.6086645329540916No Hit
AACATTCAACGCTGTCGGTGAG2487502.2310881754748384No Hit
TATTGCACTTGTCCCGGCCTGTAT1961901.7596670920458635No Hit
TAGCTTATCAGACTGATGTTGACT1937421.7377104936395822No Hit
TATTGCACTTGTCCCGGCCTGTAA1881231.6873125661702633No Hit
TAGCTTATCAGACTGATGTTGAC1839711.6500724531870612No Hit
GGAATACCGGGTGCTGTAGGCTTT1628401.460544315555066No Hit
TAGCTTATCAGACTGATGTTGA1446961.2978071744261597No Hit
TATTGCACTTGTCCCGGCCTGT1392331.2488084419533192No Hit
TGAGGTAGTAGGTTGTATAGTT1211531.0866453295409169No Hit
GGAATACCGGGTGCTGTAGGCTT1193591.070554587081412No Hit
TGTAAACATCCTTGACTGGAAGCT987080.8853316648231974No Hit
TCGTACGACTCTTAGCGG953300.8550337116302164No Hit
TATTGCACTTGTCCCGGCCTGTA941110.8441002584205529No Hit
CATAAAGTAGAAAGCACTACT865030.7758625947461304No Hit
TGAGGTAGTAGATTGTATAGTT802070.7193925197600416No Hit
AGCAGCATTGTACAGGGCTATGA744800.668025918831622No Hit
GCCTGTCTGAGCGTCGCT737290.6612900506113943No Hit
ATCCCGGACGAGCCCCCA681810.611528936249447No Hit
TGTAAACATCCCCGACTGGAAGCT627920.5631939244800644No Hit
ACTGGACTTGGAGTCAGAAGGC611040.5480539170822694No Hit
CCCATAAAGTAGAAAGCACTAC577660.5181147318371035No Hit
TTCAAGTAATTCAGGATAGGTT574180.5149934506911126No Hit
CAACGGAATCCCAAAAGCAGCTGT551460.4946154312552178No Hit
CGCCTGTCTGAGCGTCGCT504180.45220905982347903No Hit
TTAATGCTAATCGTGATAGGGGTT501540.4498411913678997No Hit
CCCATAAAGTAGAAAGCACT456800.40971299640478637No Hit
CATTGCACTTGTCTCGGTCTGA437610.39250110410835937No Hit
TGAGGTAGTAGTTTGTACAGTT341910.30666587259360883No Hit
TGAGAACTGAATTCCATGGGTT304840.27341705302984914No Hit
TAGCTTATCAGACTGATGTTGACA297790.2670937679496089No Hit
TTCTCACTACTGCACTTGACTAG287570.2579272468829344No Hit
TGTAAACATCCTACACTCTCAGCT286340.2568240354434031No Hit
CACCCGGCTGTGTGCACATGTGC285590.2561513455412499No Hit
GGAATACCGGGTGCTGTAGGCTTA278110.24944238491710846No Hit
CACTAGATTGTGAGCTCCTGGA273490.24529861511984463No Hit
AGCTCGGTCTGAGGCCCCTCAGT267910.2402938022478247No Hit
TGAATACCGGGTGCTGTAGGCTTT261440.23449073069191625No Hit
TATTGCACTTGTCCCGGCCTGTAG258020.2314232647380976No Hit
CAACACCAGTCGATGGGCTGT251800.2258444231495736No Hit
AACATTCAACGCTGTCGGTGAGTT250270.22447213574918104No Hit
TATTGCACTTGTCCCGGCCTGTT249970.22420305978831973No Hit
CACTAGATTGTGAGCTCCTGGAA246150.22077682588668598No Hit
ACCGGGTGCTGTAGGCTT242690.21767348313808582No Hit
CTCCCACTGCTTCACTTGACTAG238100.21355662093690814No Hit
AACGGAATCCCAAAAGCAGCTG233010.20899129880096165No Hit
TGTCAGTTTGTCAAATACCCCAA231760.2078701489640396No Hit
TGAGGTAGTAGTTTGTGCTGTT230360.20661446114668697No Hit
TAGCAGCACGTAAATATTGGC216300.19400376778098796No Hit
ACCGGGTGCTGTAGGCTTA212530.19062237987283112No Hit
TCGTTTCCCGGGCGGCGCACCA206320.18505250748300248No Hit
CTTCTCACTACTGCACTTGACTAG205200.18404795722912032No Hit
CAAAGAATTCTCCTTTTGGGCT198500.17803859410321826No Hit
TGAATACCGGGTGCTGTAGGCTT194750.17467514459245218No Hit
CCCCCACTGCTAAATTTGACTGGCTT191390.17166149383080576No Hit
TTCAAGTAATCCAGGATAGGCTT186150.1669616337144286No Hit
GGAATACCGGGTGCTGTAGGCT184630.1655983155127314No Hit
TTCACAGTGGCTAAGTTCCG175590.15749015989211132No Hit
ACTGGACTTGGAGTCAGAAGGCT173360.15549002858304242No Hit
ACTGGACTTGGAGTCAGAAGGCA171730.15402804919569607No Hit
ATCCCACCGCTGCCACCA170400.15283514576921103No Hit
TGTAAACATCCCCGACTGGAAGC168320.15096955244057278No Hit
ACCGGGTGCTGTAGGCTTT167430.1501712937566843No Hit
AACCGGGCGGAAACACCA166350.14920262029758366No Hit
CCCCCCACTGCTAAATTTGACTGG166100.14897839033019927No Hit
TTCAAGTAATCCAGGATAGGC162760.1459826779659436No Hit
TATTGCACTTGTCCCGGCCTGTTT160400.1438659470738348No Hit
AGCAGCATTGTACAGGGCTATGT160070.1435699635168874No Hit
CCCCCCACTGCTAAATTTGACTGT155390.1393723785274513No Hit
CAACGGAATCCCAAAAGCAGCT155350.1393365017326698No Hit
TTATAAAGCAATGAGACTGATT152010.13634078936841415No Hit
CAAAGAATTCTCCTTTTGGGCTT151470.1358564526388638No Hit
CTACGCCTGTCTGAGCGTCGCT150440.13493262517324006No Hit
ACCACAGGGTAGAACCACGGAC149750.1343137504632591No Hit
ACTGGACTTGGAGTCAGAAGG149060.13369487575327815No Hit
TGTCAGTTTGTCAAATACCCCA148630.13330920020937698No Hit
TGAGAACTGAATTCCATAGGCTGT147510.13230464995549485No Hit
ACCCCCCACTGCTAAATTTGACTG146610.13149742207291096No Hit
TTCACAGTGGCTAAGTTCCGC142370.12769448182607146No Hit
TACGCCTGTCTGAGCGTCGCT141830.12721014509652115No Hit
CCCCCCACTGCTAAATTTGACTGGCT141710.12710251471217662No Hit
TTCACAGTGGCTAAGTTCTG141660.12705766871869975No Hit
TGAGGTAGTAGGTTGTGTGGTT140340.12587373449091008No Hit
TATTGCACTTGTCCCGGCCTGTAAT138570.12428618632182849No Hit
CCCATAAAGTAGAAAGCACTA136200.12216048623102432No Hit
TTCACAGTGGCTAAGTTCTGC135690.12170305709756013No Hit
AGCAGCATTGTACAGGGCTATG124520.11168446215482487No Hit
CCCCCCACTGCTAAATTTGACTGTA122210.10961257725619297No Hit
CAAAGTGCTGTTCGTGCAGGTAG119630.10729852399278589No Hit
TATTGCACTTGTCCCGGCCTGA116930.1048768403450343No Hit
TCCTGTCTGAGCGTCGCT116830.10478714835808055No Hit
TGAGGTAGTAGGTTGTATAGT116250.10426693483374873No Hit
TAGCAGCACGTAAATATTGGCGT116010.1040516740650597No Hit
TATTGCACTTGTCCCGGCCTGTAAA113030.10137885285383759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)