FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6763481
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG72788510.761987798886402No Hit
CCCCCCACTGCTAAATTTGACTG5741478.488927521198034No Hit
AACATTCAACGCTGTCGGTGAGT2541403.757532548697926No Hit
AAGCTGCCAGTTGAAGAACTGT2533903.7464435843022255No Hit
TTCAAGTAATCCAGGATAGGCT2436283.602109623727782No Hit
TAGCAGCACGTAAATATTGGCG2166723.203557458060428No Hit
CCCCCACTGCTAAATTTGACTG1922902.8430626181991197No Hit
TAGCTTATCAGACTGATGTTGACT1775472.6250831487513606No Hit
TAGCTTATCAGACTGATGTTGAC1587372.3469719217071803No Hit
AACATTCAACGCTGTCGGTGAG1562822.3106740449185854No Hit
TATTGCACTTGTCCCGGCCTGTAA1242561.8371604799362933No Hit
TAGCTTATCAGACTGATGTTGA1084351.6032424723304466No Hit
TATTGCACTTGTCCCGGCCTGTAT1046541.5473393064902525No Hit
TGAGGTAGTAGGTTGTATAGTT872191.2895578475048572No Hit
TGTAAACATCCTTGACTGGAAGCT778751.1514041364202843No Hit
TGAGGTAGTAGATTGTATAGTT614100.9079644047199955No Hit
CATAAAGTAGAAAGCACTACT512800.7581894589487277No Hit
TATTGCACTTGTCCCGGCCTGT468020.6919809488634625No Hit
CAACGGAATCCCAAAAGCAGCTGT463540.6853571407977638No Hit
CCCATAAAGTAGAAAGCACTAC458130.6773583011469981No Hit
TGTAAACATCCCCGACTGGAAGCT454630.672183451095671No Hit
TTCAAGTAATTCAGGATAGGTT436970.6460726362652605No Hit
TTAATGCTAATCGTGATAGGGGTT433950.6416074799352582No Hit
AGCAGCATTGTACAGGGCTATGA406680.6012880053924895No Hit
TATTGCACTTGTCCCGGCCTGTA397000.5869758486791048No Hit
ACTGGACTTGGAGTCAGAAGGC301520.4458059392789009No Hit
TAGCTTATCAGACTGATGTTGACA298200.44089722437307066No Hit
TGAGGTAGTAGTTTGTACAGTT272990.40362351871765445No Hit
CAAAGAATTCTCCTTTTGGGCT252410.373195400415851No Hit
CCCATAAAGTAGAAAGCACT244920.3621212213060109No Hit
TGTCAGTTTGTCAAATACCCCAA232530.3438022521243129No Hit
TGTAAACATCCTACACTCTCAGCT216550.32017536531853935No Hit
TTCTCACTACTGCACTTGACTAG197540.29206853689690265No Hit
GGAATACCGGGTGCTGTAGGCTTT196260.290176020306703No Hit
CATTGCACTTGTCTCGGTCTGA192940.2852673054008727No Hit
AACATTCAACGCTGTCGGTGAGTT181760.2687373558083478No Hit
CCCCCACTGCTAAATTTGACTGGCTT181210.2679241650859964No Hit
CAAAGAATTCTCCTTTTGGGCTT168510.24914685204260942No Hit
CTTCTCACTACTGCACTTGACTAG151520.22402665136488148No Hit
CACCCGGCTGTGTGCACATGTGC143580.21228713439129937No Hit
TTCAAGTAATCCAGGATAGGCTT141470.20916743907464216No Hit
TGTCAGTTTGTCAAATACCCCA137850.20381516559298385No Hit
TATTGCACTTGTCCCGGCCTGTAG137120.20273583972513562No Hit
TGAGGTAGTAGTTTGTGCTGTT134980.19957178855089563No Hit
TGATGTAGTAGGTTGTATAGTT134010.19813761582238493No Hit
TAGCAGCACGTAAATATTGGC129830.19195736633251428No Hit
CACTAGATTGTGAGCTCCTGGAA129010.19074497289191764No Hit
ACCCCCCACTGCTAAATTTGACTG122410.1809866842237008No Hit
CACTAGATTGTGAGCTCCTGGA119220.17627017803406264No Hit
AACGGAATCCCAAAAGCAGCTG117320.17346097372048505No Hit
TGTAAACATCCCCGACTGGAAGC115710.1710805426968746No Hit
GGAATACCGGGTGCTGTAGGCTT115300.17047434597657626No Hit
TATTGCACTTGTCCCGGCCTGTAAT109040.1612187570276312No Hit
TTATAAAGCAATGAGACTGATT106520.15749286499067566No Hit
ATGCACCTGGGCAAGGATTCTGA102300.15125347435736125No Hit
CCCCCCACTGCTAAATTTGACTGGCT99350.14689181502838553No Hit
CCCCCCACTGCTAAATTTGACTGTA99230.1467143915980543No Hit
CCCCCCACTGCTAAATTTGACTGT98790.14606383902017317No Hit
ACTGGACTTGGAGTCAGAAGGCT98740.14598991259086852No Hit
CCCCCCACTGCTAAATTTGACTGG97600.14430439000272197No Hit
TATTGCACTTGTCCCGGCCTGTT96900.14326941999245654No Hit
ACTGGACTTGGAGTCAGAAGGCA96880.14323984942073467No Hit
TGATGTAGTAGATTGTATAGTT96830.14316592299143002No Hit
TTCAAGTAATCCAGGATAGGC96530.14272236441560196No Hit
TATTGCACTTGTCCCGGCCTGTAAA96000.1419387442649724No Hit
CTCCCACTGCTTCACTTGACTAG95850.1417169649770584No Hit
TTCACAGTGGCTAAGTTCCG95480.14116990940020382No Hit
TTCACAGTGGCTAAGTTCTGC94640.1399279453878853No Hit
CGCCTGTCTGAGCGTCGCT93970.1389373312352027No Hit
TTCACAGTGGCTAAGTTCCGC92000.13602462992059858No Hit
CAACACCAGTCGATGGGCTGT90280.13348156075251782No Hit
AGCTCGGTCTGAGGCCCCTCAGT87270.12903118970837651No Hit
CAACGGAATCCCAAAAGCAGCT86860.1284249929880782No Hit
TTCACAGTGGCTAAGTTCTG85860.1269464644019847No Hit
TTAATGCTAATCGTGATAGGGGTTT85410.12628112653824264No Hit
CCCATAAAGTAGAAAGCACTA85270.12607413253618957No Hit
ATCACATTGCCAGGGATTTCC84670.1251870153845335No Hit
TTTAAACATCCTTGACTGGAAGCT82840.12248130807198246No Hit
ATCACATTGCCAGGGATTTCCA82220.12156462034860452No Hit
TGAGAACTGAATTCCATGGGTT81310.12021915933525945No Hit
TATTGCACTTGTCCCGGCCTGTTT78890.11664112015691329No Hit
TAGCAGCACATCATGGTTTACA75960.11230903139965943No Hit
TGAGGTAGTAAGTTGTATTGTT75800.11207246682588448No Hit
AGCAGCATTGTACAGGGCTATGT75470.11158455239247364No Hit
TAGCAGCACGTAAATATTGGCGT73390.10850921293339923No Hit
TAGCTTATCAGACTGATGTTGACC72480.10716375192005417No Hit
CAAAGTGCTGTTCGTGCAGGTAG71850.1062322789108153No Hit
CTACGCCTGTCTGAGCGTCGCT71270.1053747323308811No Hit
AACATTCAACGCTGTCGGTGA69950.10342307459723774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)