FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7582868
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG86382811.391837494731545No Hit
CCCCCCACTGCTAAATTTGACTG7269299.586465173862976No Hit
TAGCAGCACGTAAATATTGGCG2562923.379882123755814No Hit
CCCCCACTGCTAAATTTGACTG2524603.3293471546649633No Hit
AACATTCAACGCTGTCGGTGAGT2482083.2732733841601886No Hit
TTCAAGTAATCCAGGATAGGCT2163632.8533135483830128No Hit
AAGCTGCCAGTTGAAGAACTGT2150912.836538892672271No Hit
AACATTCAACGCTGTCGGTGAG1961792.587134577576716No Hit
TAGCTTATCAGACTGATGTTGACT1688692.2269806094475073No Hit
TAGCTTATCAGACTGATGTTGAC1660132.189316759832823No Hit
TATTGCACTTGTCCCGGCCTGTAA1319081.7395528973997703No Hit
TATTGCACTTGTCCCGGCCTGTAT1062471.4011453186314202No Hit
TAGCTTATCAGACTGATGTTGA1011131.3334400651574048No Hit
TGTAAACATCCTTGACTGGAAGCT757300.9986986454201761No Hit
TGAGGTAGTAGGTTGTATAGTT624250.8232373292005083No Hit
AGCAGCATTGTACAGGGCTATGA497020.6554512092258497No Hit
TATTGCACTTGTCCCGGCCTGT483200.6372259150495565No Hit
TATTGCACTTGTCCCGGCCTGTA480610.6338103208443032No Hit
TGTAAACATCCCCGACTGGAAGCT469520.6191852475870607No Hit
CAACGGAATCCCAAAAGCAGCTGT460480.6072636369247097No Hit
CATAAAGTAGAAAGCACTACT432270.5700613540945194No Hit
TTCAAGTAATTCAGGATAGGTT373470.49251813429958163No Hit
TAGCTTATCAGACTGATGTTGACA364940.48126909238035004No Hit
ACTGGACTTGGAGTCAGAAGGC356140.46966398465593756No Hit
TGAGGTAGTAGATTGTATAGTT328130.4327254542740293No Hit
CAACGGAATCCCAAAAGCAGCT323240.426276706913532No Hit
CCCATAAAGTAGAAAGCACTAC284260.3748713547433504No Hit
TGTCAGTTTGTCAAATACCCCAA261370.3446848870374639No Hit
CCCATAAAGTAGAAAGCACT235250.3102388172918215No Hit
CAAAGAATTCTCCTTTTGGGCT232070.3060451533641361No Hit
CCCCCACTGCTAAATTTGACTGGCTT225710.2976578255087653No Hit
CATTGCACTTGTCTCGGTCTGA210080.2770455716755191No Hit
TGTAAACATCCTACACTCTCAGCT210000.27694007069620624No Hit
CACCCGGCTGTGTGCACATGTGC199930.26366013492520246No Hit
TTCTCACTACTGCACTTGACTAG197310.2602049778527069No Hit
AACATTCAACGCTGTCGGTGAGTT191590.2526616578318388No Hit
CGCCTGTCTGAGCGTCGCT191510.25255615685252597No Hit
CTTCTCACTACTGCACTTGACTAG184450.2432456954281678No Hit
TCGTTTCCCGGCCAATGCACCA177000.23342091672965953No Hit
TAGCAGCACGTAAATATTGGC171610.2263127882484569No Hit
ACCCCCCACTGCTAAATTTGACTG169320.22329282271562687No Hit
CACTAGATTGTGAGCTCCTGGAA164410.2168177001103013No Hit
TGAGGTAGTAGTTTGTACAGTT160350.21146352541017463No Hit
AACGGAATCCCAAAAGCAGCTG153790.2028124451065217No Hit
TGTCAGTTTGTCAAATACCCCA149430.1970626417339719No Hit
TATTGCACTTGTCCCGGCCTGTAG147630.19468886969943297No Hit
CACTAGATTGTGAGCTCCTGGA143990.18988857514069876No Hit
ACTGGACTTGGAGTCAGAAGGCA135450.17862634559905302No Hit
CAAAGAATTCTCCTTTTGGGCTT135300.17842853126284144No Hit
CTACGCCTGTCTGAGCGTCGCT130510.17211166012648513No Hit
CAACACCAGTCGATGGGCTGT129790.17116215131266957No Hit
TGTAAACATCCCCGACTGGAAGC128830.16989613956091548No Hit
GGAATACCGGGTGCTGTAGGCTTT128350.16926313368503842No Hit
ATGCACCTGGGCAAGGATTCTGA126600.16695529976257006No Hit
CCCCCCACTGCTAAATTTGACTGG124280.16389577136249767No Hit
TATTGCACTTGTCCCGGCCTGTAAA124010.16353970555731684No Hit
ACTGGACTTGGAGTCAGAAGGCT122880.16204950422452297No Hit
TTCAAGTAATCCAGGATAGGCTT115910.15285773140189174No Hit
TTCACAGTGGCTAAGTTCCG114570.15109058999840166No Hit
TATTGCACTTGTCCCGGCCTGTAAT110500.1457232276758609No Hit
CTCCCACTGCTTCACTTGACTAG107430.14167462759473065No Hit
TATTGCACTTGTCCCGGCCTGTT107360.1415823142378319No Hit
TGAGGTAGTAGTTTGTGCTGTT102930.13574019750838337No Hit
TCGTACGACTCTTAGCGG102670.13539731932561663No Hit
TTCACAGTGGCTAAGTTCTG99500.13121684302034534No Hit
CCCCCCACTGCTAAATTTGACTGGCT98390.12975301693237967No Hit
ATCACATTGCCAGGGATTTCC97790.12896175958753336No Hit
TGATGTAGTAGGTTGTATAGTT97430.12848700518062559No Hit
AGCAGCATTGTACAGGGCTATGT94530.12466259468053513No Hit
TTCACAGTGGCTAAGTTCTGC91550.12073268320113183No Hit
ATCACATTGCCAGGGATTTCCA91380.12050849362009203No Hit
CAACGGAATCCCAAAAGCAGCTGA91290.12038980501836508No Hit
TACGCCTGTCTGAGCGTCGCT90750.11967767340800341No Hit
CCCCCCACTGCTAAATTTGACTGTA90360.1191633561338533No Hit
TTATAAAGCAATGAGACTGATT89850.11849078739073396No Hit
TTCAAGTAATCCAGGATAGGC89730.1183325359217647No Hit
GCCTGTCTGAGCGTCGCT89680.11826659780969416No Hit
GGAATACCGGGTGCTGTAGGCTT87200.11499606745099611No Hit
CCCCCCACTGCTAAATTTGACTGT86750.11440262444236138No Hit
ACCACAGGGTAGAACCACGGAC84660.11164641135781343No Hit
CCCATAAAGTAGAAAGCACTA83440.11003752142329261No Hit
CAAAGTGCTGTTCGTGCAGGTAG83240.1097737689750105No Hit
CGCCCCACTGCTAAATTTGACTG82050.108204441907732No Hit
TAGCAGCACGTAAATATTGGCGT80740.10647686337148425No Hit
TAGCAGCACATCATGGTTTACA80400.10602848420940467No Hit
TTCACAGTGGCTAAGTTCCGC78950.10411627895935945No Hit
TTTAAACATCCTTGACTGGAAGCT78380.10336458448175546No Hit
ACTTGACTTGGAGTCAGAAGGC77990.10285026720760536No Hit
ACTGGACTTGGAGTCAGAAGG77460.10215132321965778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)