FastQCFastQC Report
Thu 15 Sep 2022
EGAF00003947077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792857
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG88165410.026934362744669No Hit
CCCCCCACTGCTAAATTTGACTG7889508.972624028799741No Hit
AACATTCAACGCTGTCGGTGAGT3999344.548396499567774No Hit
TTCAAGTAATCCAGGATAGGCT2964973.372021175824877No Hit
AAGCTGCCAGTTGAAGAACTGT2652723.0169033796409974No Hit
TAGCAGCACGTAAATATTGGCG2572852.9260682847452197No Hit
AACATTCAACGCTGTCGGTGAG2527952.8750041084484828No Hit
CCCCCACTGCTAAATTTGACTG2353782.676922870461785No Hit
TATTGCACTTGTCCCGGCCTGTAA1561521.7758960483492452No Hit
TATTGCACTTGTCCCGGCCTGTAT1456991.6570154615274648No Hit
TGAGGTAGTAGGTTGTATAGTT1334401.5175954755092684No Hit
TAGCTTATCAGACTGATGTTGACT1153491.3118489246441742No Hit
TAGCTTATCAGACTGATGTTGAC1085151.2341267462896301No Hit
TATTGCACTTGTCCCGGCCTGT1064241.2103460797781653No Hit
TAGCTTATCAGACTGATGTTGA939881.068913096164307No Hit
TGAGGTAGTAGATTGTATAGTT755290.8589813299590793No Hit
CATAAAGTAGAAAGCACTACT703540.8001267392384523No Hit
TATTGCACTTGTCCCGGCCTGTA688820.7833858778779185No Hit
TCGTACGACTCTTAGCGG665650.7570349432499585No Hit
TGTAAACATCCTTGACTGGAAGCT636100.7234281189833975No Hit
AGCAGCATTGTACAGGGCTATGA535320.6088123575761553No Hit
TTCAAGTAATTCAGGATAGGTT532510.6056165817321947No Hit
ACTGGACTTGGAGTCAGAAGGC530260.6030576864834718No Hit
TGTAAACATCCCCGACTGGAAGCT505240.5746027713176729No Hit
CAACGGAATCCCAAAAGCAGCTGT487090.5539610163113081No Hit
CCCATAAAGTAGAAAGCACTAC440960.5014979772786023No Hit
CAACGGAATCCCAAAAGCAGCT379650.431770924967846No Hit
CATTGCACTTGTCTCGGTCTGA370880.42179691993171275No Hit
CCCATAAAGTAGAAAGCACT334770.38072949440665305No Hit
TGAGGTAGTAGTTTGTACAGTT321280.3653874957820877No Hit
TTCTCACTACTGCACTTGACTAG319150.36296507494663No Hit
CACCCGGCTGTGTGCACATGTGC290240.3300861142174836No Hit
CAACACCAGTCGATGGGCTGT285260.32442242606697685No Hit
CTCCCACTGCTTCACTTGACTAG278140.31632494421324037No Hit
CTTCTCACTACTGCACTTGACTAG274650.31235581336077684No Hit
CGCCTGTCTGAGCGTCGCT273140.3106385103271895No Hit
ACCGGGTGCTGTAGGCTT263500.2996750657948833No Hit
AACATTCAACGCTGTCGGTGAGTT262040.2980146271001564No Hit
AACGGAATCCCAAAAGCAGCTG212680.24187815177706176No Hit
ACCGGGTGCTGTAGGCTTA198880.22618359425156123No Hit
TGTAAACATCCTACACTCTCAGCT196960.22400000363931769No Hit
CACTAGATTGTGAGCTCCTGGA187480.21321852499136515No Hit
TATTGCACTTGTCCCGGCCTGTT184010.20927213987444582No Hit
CCCCCACTGCTAAATTTGACTGGCTT182840.20794151434510988No Hit
TATTGCACTTGTCCCGGCCTGTAG175510.19960520226815925No Hit
CCCCCCACTGCTAAATTTGACTGG172630.1963298163497939No Hit
TGAGGTAGTAGTTTGTGCTGTT170600.19402112419205725No Hit
TTCAAGTAATCCAGGATAGGCTT168660.1918147878442695No Hit
ACCCCCCACTGCTAAATTTGACTG160220.18221608744461557No Hit
TCGTTTCCCGGCCAATGCACCA159070.18090820765082383No Hit
CACTAGATTGTGAGCTCCTGGAA156650.1781559736499752No Hit
ACCGGGTGCTGTAGGCTTT155860.17725751709597917No Hit
TAGCAGCACGTAAATATTGGC155050.1763363148064389No Hit
ACTGGACTTGGAGTCAGAAGGCT154750.17599512877327583No Hit
ATCCCGGACGAGCCCCCA152210.173106420359162No Hit
TTCAAGTAATCCAGGATAGGC146130.16619171675372407No Hit
CCCCCCACTGCTAAATTTGACTGGCT145880.16590739505942154No Hit
TGTAAACATCCCCGACTGGAAGC141320.1607213673553431No Hit
ACTGGACTTGGAGTCAGAAGGCA138850.15791226901563396No Hit
TGAGGTAGTAGGTTGTGTGGTT134580.15305605447694645No Hit
CAAAGAATTCTCCTTTTGGGCT134570.15304468160917437No Hit
CACCCGTAGAACCGACCTTGCG131180.14918927943443183No Hit
TAGCTTATCAGACTGATGTTGACA128300.1459138935160665No Hit
AGCTCGGTCTGAGGCCCCTCAGT127310.14478797960662843No Hit
TTCACAGTGGCTAAGTTCCGC122650.13948822322482896No Hit
TGAGGTAGTAGGTTGTATAGT120770.1373501240836738No Hit
ACCACAGGGTAGAACCACGGAC119240.13561007531454225No Hit
AACATTCAACGCTGTCGGTGA117960.13415434823971323No Hit
TTCACAGTGGCTAAGTTCTGC117870.13405199242976432No Hit
TTATAAAGCAATGAGACTGATT117270.1333696203634382No Hit
ACTGGACTTGGAGTCAGAAGG116740.1327668583715168No Hit
AGCAGCATTGTACAGGGCTATGT116350.13232331652840482No Hit
TATTGCACTTGTCCCGGCCTGTAAT115190.131004063866841No Hit
TATTGCACTTGTCCCGGCCTGTTT114060.1297189298085935No Hit
CAACGGAATCCCAAAAGCAGCTGA112030.12741023765085682No Hit
CAAAGAATTCTCCTTTTGGGCTT111700.12703493301437746No Hit
CCCATAAAGTAGAAAGCACTA110920.12614784932815354No Hit
CGCGACCTCAGATCAGACGTGGCG110300.1254427315262832No Hit
CTCCCACTGCTTCACTTGACTAGCCT109360.12437368195570564No Hit
TTCACAGTGGCTAAGTTCCG108580.12348659826948169No Hit
TTCACAGTGGCTAAGTTCTG99170.11278472969593387No Hit
AGAGGTAGTAGGTTGCATAGTT98590.11212510336515197No Hit
TGATGTAGTAGGTTGTATAGTT98070.11153371424100265No Hit
AACATTCAACGCTGTCGGTGAGA97110.1104419189348809No Hit
AGCAGCATTGTACAGGGCTATG94370.10732575316532499No Hit
CCCCCCACTGCTAAATTTGACTGT93980.10688221132221301No Hit
TGTCAGTTTGTCAAATACCCCAA93020.10579041601609124No Hit
CAAAGTGCTGTTCGTGCAGGT91530.10409585871804806No Hit
ATCCCACCGCTGCCACCA91530.10409585871804806No Hit
TATTGCACTTGTCCCGGCCTGA91470.10402762151141545No Hit
CTACGCCTGTCTGAGCGTCGCT90280.10267425024653534No Hit
CAAAGTGCTGTTCGTGCAGGTAG90120.1024922843621817No Hit
TCGTTTCCCGGGCGGCGCACCA88900.10110479449398528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)