FastQCFastQC Report
Thu 15 Sep 2022
EGAF00003947079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8695326
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8394079.653542604383091No Hit
CCCCCCACTGCTAAATTTGACTG5118965.887024822301084No Hit
AACATTCAACGCTGTCGGTGAGT3442803.959368515913032No Hit
AAGCTGCCAGTTGAAGAACTGT2754443.1677248213580493No Hit
TAGCAGCACGTAAATATTGGCG2653213.051305954486353No Hit
TTCAAGTAATCCAGGATAGGCT2647223.044417196089025No Hit
AACATTCAACGCTGTCGGTGAG2332542.682521621386018No Hit
TATTGCACTTGTCCCGGCCTGTAA1662231.9116362054740672No Hit
CCCCCACTGCTAAATTTGACTG1595061.8343878078866738No Hit
TATTGCACTTGTCCCGGCCTGTAT1504911.730711418985326No Hit
TAGCTTATCAGACTGATGTTGACT1372781.5787562191457802No Hit
TAGCTTATCAGACTGATGTTGAC1315641.5130427542337113No Hit
TGAGGTAGTAGGTTGTATAGTT1023831.1774486660994654No Hit
TAGCTTATCAGACTGATGTTGA994241.1434188896425506No Hit
TCGTACGACTCTTAGCGG989791.138301197677925No Hit
TATTGCACTTGTCCCGGCCTGT948281.090562907014642No Hit
TGTAAACATCCTTGACTGGAAGCT760620.8747458117153973No Hit
TATTGCACTTGTCCCGGCCTGTA672180.7730359965802317No Hit
TGAGGTAGTAGATTGTATAGTT612200.7040564091559075No Hit
CATAAAGTAGAAAGCACTACT607330.6984556990732723No Hit
TTCAAGTAATTCAGGATAGGTT593710.6827921115321035No Hit
AGCAGCATTGTACAGGGCTATGA573800.6598947526521719No Hit
TGTAAACATCCCCGACTGGAAGCT561330.6455537147198391No Hit
TTAATGCTAATCGTGATAGGGGTT528730.6080623084171888No Hit
CAACGGAATCCCAAAAGCAGCTGT504190.5798402498077703No Hit
ACTGGACTTGGAGTCAGAAGGC424170.48781379789555906No Hit
TGAGAACTGAATTCCATGGGTT395200.4544970481842774No Hit
CCCATAAAGTAGAAAGCACTAC378030.43475080750278944No Hit
TGAGAACTGAATTCCATAGGCTGT363130.41761516474482957No Hit
CAACGGAATCCCAAAAGCAGCT340400.3914746842154049No Hit
CATTGCACTTGTCTCGGTCTGA326170.3751095703599842No Hit
CCCATAAAGTAGAAAGCACT275130.31641136859043584No Hit
TGAGGTAGTAGTTTGTACAGTT261930.30123079916727674No Hit
TGTAAACATCCTACACTCTCAGCT248570.28586622284201885No Hit
TTCTCACTACTGCACTTGACTAG247140.2842216611545099No Hit
CACCCGGCTGTGTGCACATGTGC235120.2703981426343302No Hit
AACATTCAACGCTGTCGGTGAGTT227360.26147380788253366No Hit
GCCTGTCTGAGCGTCGCT217400.25001937822687725No Hit
CTTCTCACTACTGCACTTGACTAG208620.2399219994742003No Hit
CAACACCAGTCGATGGGCTGT203720.2342867880974215No Hit
CAAAGAATTCTCCTTTTGGGCT203530.2340682799011791No Hit
TGATGTAGTAGGTTGTATAGTT202890.23333225229278354No Hit
TATTGCACTTGTCCCGGCCTGTAG199160.22904259138760297No Hit
TCGTTTCCCGGCCAATGCACCA192340.22119929718563744No Hit
ACCGGGTGCTGTAGGCTT190700.2193132264391237No Hit
CACTAGATTGTGAGCTCCTGGA189460.21788717294785728No Hit
TAGCTTATCAGACTGATGTTGACA188790.21711664404531814No Hit
TATTGCACTTGTCCCGGCCTGTT179360.20627173725286438No Hit
CACTAGATTGTGAGCTCCTGGAA179320.20622573552733964No Hit
CCCCCACTGCTAAATTTGACTGGCTT178010.2047191790164049No Hit
TAGCAGCACGTAAATATTGGC175440.20176356815144136No Hit
CACCCGTAGAACCGACCTTGCG169460.19488631018549507No Hit
AACGGAATCCCAAAAGCAGCTG167600.1927472299485954No Hit
TGTAAACATCCCCGACTGGAAGC162110.18643349312032695No Hit
TTCAAGTAATCCAGGATAGGCTT160410.18447841978552615No Hit
CTCCCACTGCTTCACTTGACTAG156170.17960223687990537No Hit
ATCCCGGACGAGCCCCCA155160.17844069331040607No Hit
TGAGGTAGTAGTTTGTGCTGTT154100.17722164758400089No Hit
AGCTCGGTCTGAGGCCCCTCAGT151560.17430053801318088No Hit
TACGCCTGTCTGAGCGTCGCT150640.17324249832611222No Hit
CCCCCCACTGCTAAATTTGACTGG145660.16751528349828404No Hit
ACCGGGTGCTGTAGGCTTA144320.16597422569320575No Hit
CAAAGAATTCTCCTTTTGGGCTT143830.1654107045555279No Hit
ACTGGACTTGGAGTCAGAAGGCA138520.1593039754921207No Hit
ACCCCCCACTGCTAAATTTGACTG135020.15527882450870734No Hit
ACTGGACTTGGAGTCAGAAGGCT133730.15379526886053496No Hit
TATTGCACTTGTCCCGGCCTGTTT131230.1509201610152397No Hit
TATTGCACTTGTCCCGGCCTGTAAT131120.15079365627004668No Hit
AGCAGCATTGTACAGGGCTATGT130850.1504831446227548No Hit
CGCCTGTCTGAGCGTCGCT127010.14606697897238125No Hit
TCGTTTCCCGGGCGGCGCACCA123640.1421913335969232No Hit
TTCAAGTAATCCAGGATAGGC122390.1407537796742756No Hit
TATTGCACTTGTCCCGGCCTGTAAA121700.1399602499089741No Hit
TGATGTAGTAGATTGTATAGTT121420.139638237830301No Hit
ACCGGGTGCTGTAGGCTTT116320.13377301782589865No Hit
CTACGCCTGTCTGAGCGTCGCT115440.1327609798643547No Hit
TTATAAAGCAATGAGACTGATT111980.12878183060646603No Hit
TGTCAGTTTGTCAAATACCCCAA109170.12555020938835415No Hit
TGAGAACTGAATTCCATGGGTTG107420.12353763389664747No Hit
GGAATACCGGGTGCTGTAGGCTTT102730.11814393157887353No Hit
GGAATACCGGGTGCTGTAGGCTT102460.11783341993158163No Hit
AACATTCAACGCTGTCGGTGA100840.1159703500478303No Hit
TGTCAGTTTGTCAAATACCCCA100420.11548733192982069No Hit
TTCACAGTGGCTAAGTTCCGC100390.11545283063567714No Hit
CCCATAAAGTAGAAAGCACTA99660.11461329914485092No Hit
TTCACAGTGGCTAAGTTCCG97810.11248571933933241No Hit
CGTTCGCGCTTTCCCCTG95380.10969111451370542No Hit
CCCCCCACTGCTAAATTTGACTGGCT93950.1080465528261965No Hit
CGCGACCTCAGATCAGACGTGGCG92990.10694251141360313No Hit
ACTGGACTTGGAGTCAGAAGG92790.10671250278597949No Hit
ATGCACCTGGGCAAGGATTCTGA91700.10545895576543075No Hit
ACCACAGGGTAGAACCACGGAC90960.10460792384322337No Hit
TTCACAGTGGCTAAGTTCTGC90830.10445841823526801No Hit
TGAGGTAGTAGGTTGTGTGGTT90730.1043434139214562No Hit
AACATTCAACGCTGTCGGTGAGA89620.10306686603814509No Hit
TCCTGTCTGAGCGTCGCT88860.10219283325317532No Hit
TAGCAGCACGTAAATATTGGCGT87870.1010542905464384No Hit
ACTTGACTTGGAGTCAGAAGGC87370.10047926897737934No Hit
CAAAGTGCTGTTCGTGCAGGTAG87130.100203258624231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)