FastQCFastQC Report
Thu 15 Sep 2022
EGAF00003947080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5669389
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4751538.381026597398767No Hit
CCCCCCACTGCTAAATTTGACTG3346725.903140532427745No Hit
AACATTCAACGCTGTCGGTGAGT2554564.505882379917836No Hit
AAGCTGCCAGTTGAAGAACTGT1847563.2588344176065536No Hit
TTCAAGTAATCCAGGATAGGCT1792933.162474827534325No Hit
AACATTCAACGCTGTCGGTGAG1747723.0827307845695544No Hit
TAGCAGCACGTAAATATTGGCG1588262.801465907525485No Hit
CCCCCACTGCTAAATTTGACTG1207682.1301766380821636No Hit
TGAGGTAGTAGGTTGTATAGTT850591.5003204048972474No Hit
TAGCTTATCAGACTGATGTTGAC752641.3275504644327634No Hit
TATTGCACTTGTCCCGGCCTGT722161.273788057231564No Hit
TATTGCACTTGTCCCGGCCTGTAA714461.2602063467509461No Hit
TCGTACGACTCTTAGCGG695431.226640119420276No Hit
TAGCTTATCAGACTGATGTTGACT673221.1874648220469612No Hit
TAGCTTATCAGACTGATGTTGA672841.1867945558154502No Hit
TATTGCACTTGTCCCGGCCTGTAT597141.0532704670644404No Hit
CGCCTGTCTGAGCGTCGCT546820.9645131071443501No Hit
TATTGCACTTGTCCCGGCCTGTA439470.7751628967424885No Hit
ACCGGGTGCTGTAGGCTT433650.7648972402493461No Hit
TTAATGCTAATCGTGATAGGGGTT430020.758494433879912No Hit
CATAAAGTAGAAAGCACTACT373840.6594008631265204No Hit
AGCAGCATTGTACAGGGCTATGA365990.645554573870306No Hit
TGTAAACATCCTTGACTGGAAGCT353700.6238767528564366No Hit
ACTGGACTTGGAGTCAGAAGGC349850.6170858976161276No Hit
TGAGGTAGTAGATTGTATAGTT349610.6166625715751732No Hit
TTCAAGTAATTCAGGATAGGTT265910.46902761479235244No Hit
TGTAAACATCCCCGACTGGAAGCT261700.46160177049061196No Hit
CATTGCACTTGTCTCGGTCTGA256920.4531705268416049No Hit
CAACACCAGTCGATGGGCTGT249600.4402590825924981No Hit
CAACGGAATCCCAAAAGCAGCT240510.42422560879135307No Hit
ACCGGGTGCTGTAGGCTTT228780.40353554853971035No Hit
CAACGGAATCCCAAAAGCAGCTGT226560.3996197826608828No Hit
ACCGGGTGCTGTAGGCTTA211930.3738145327477088No Hit
CCCATAAAGTAGAAAGCACT207780.3664945199562069No Hit
AACATTCAACGCTGTCGGTGAGTT181880.3208105847032193No Hit
TGAGGTAGTAGTTTGTACAGTT153320.27043478582965463No Hit
CCCATAAAGTAGAAAGCACTAC152060.26821232411464446No Hit
CACTAGATTGTGAGCTCCTGGA141310.24925084519689866No Hit
CACCCGTAGAACCGACCTTGCG135980.2398494793707047No Hit
TACGCCTGTCTGAGCGTCGCT134580.23738007746513778No Hit
TTCTCACTACTGCACTTGACTAG133400.2352987244304457No Hit
TGAGGTAGTAGTTTGTGCTGTT133090.23475192829421299No Hit
TTCACAGTGGCTAAGTTCCG131560.23205322478312918No Hit
AACGGAATCCCAAAAGCAGCTG130820.2307479694901867No Hit
TGAGAACTGAATTCCATAGGCTGT120500.21254494972915072No Hit
CTACGCCTGTCTGAGCGTCGCT119730.21118677868108893No Hit
TTCACAGTGGCTAAGTTCTG117500.20725337421722165No Hit
TATTGCACTTGTCCCGGCCTGTT114330.20166194275961657No Hit
ATCCCGGACGAGCCCCCA114190.20141500256905992No Hit
CACCCGGCTGTGTGCACATGTGC113980.20104459228322485No Hit
ACTGGACTTGGAGTCAGAAGG111890.19735812800991429No Hit
TGTAAACATCCTACACTCTCAGCT110450.19481817176418834No Hit
ACGCCTGTCTGAGCGTCGCT104540.1843937680056881No Hit
TGAGGTAGTAGGTTGTGTGGTT104140.18368822460409756No Hit
GCGTTCGCGCTTTCCCCTG102300.18044272495678107No Hit
TCGTTTCCCGGCCAATGCACCA102060.18001939891582672No Hit
CTTCTCACTACTGCACTTGACTAG100440.17716194813938504No Hit
TGAGAACTGAATTCCATGGGTT100070.1765093204929138No Hit
CACTAGATTGTGAGCTCCTGGAA99580.17564502982596536No Hit
ACTGGACTTGGAGTCAGAAGGCT96510.17022998421875796No Hit
ACTGGACTTGGAGTCAGAAGGCA90270.1592235071539455No Hit
ACCACAGGGTAGAACCACGGAC89660.15814755346651993No Hit
CTCCCACTGCTTCACTTGACTAG89140.1572303470444522No Hit
GCCTGTCTGAGCGTCGCT88690.15643661071766288No Hit
TAGCAGCACGTAAATATTGGC88370.15587217599639044No Hit
TATTGCACTTGTCCCGGCCTGTAG86570.152697230689233No Hit
CAAAGAATTCTCCTTTTGGGCT85760.15126850530101216No Hit
TTCAAGTAATCCAGGATAGGC85390.1506158776545409No Hit
TAGCTTATCAGACTGATGTTGACA85090.150086720103348No Hit
AGCAGCATTGTACAGGGCTATG84970.14987505708287083No Hit
TTCAAGTAATCCAGGATAGGCTT84950.1498397799127913No Hit
AGCTCGGTCTGAGGCCCCTCAGT83200.1467530275308327No Hit
TGAGGTAGTAGGTTGTATAGT81020.14290781599216423No Hit
AATGACACGATCACTCCCGTTG76900.13564071895578164No Hit
TTATAAAGCAATGAGACTGATT73940.13041969778401163No Hit
TGATGTAGTAGGTTGTATAGTT71330.12581602708863338No Hit
CGCGACCTCAGATCAGACGTGGCG71000.12523395378232116No Hit
CAACGGAATCCCAAAAGCAGCTGA70770.12482826632640659No Hit
AACATTCAACGCTGTCGGTGA70680.12466951906104874No Hit
CCCCCCACTGCTAAATTTGACTGG69020.12174151394444797No Hit
AGCAGCATTGTACAGGGCTATGT68570.12094777761765861No Hit
TTCACAGTGGCTAAGTTCTGC66000.11641466126243939No Hit
TGTAAACATCCCCGACTGGAAGC65700.1158855037112465No Hit
CGCGACCTCAGATCAGACGTGG65340.115250514649815No Hit
TTAATGCTAATCGTGATAGGGGT65020.11468607992854256No Hit
CAAAGTGCTGTTCGTGCAGGT64690.11410400662223037No Hit
AGCTACATTGTCTGCTGGGTTT62860.11087614555995363No Hit
CAAAGTGCTGTTCGTGCAGGTAG62290.10987074621268712No Hit
TATTGCACTTGTCCCGGCCTG60610.10690746392600685No Hit
AGCTACATCTGGCTACTGGGTCTC60040.10590206457874032No Hit
AACATTCAACGCTGTCGGTGAGA59040.10413820607476396No Hit
CTCGTACGACTCTTAGCGG57170.10083979067232818No Hit
AATGCACCCGGGCAAGGATTCT56940.1004341032164136No Hit
ACCCCCCACTGCTAAATTTGACTG56760.10011660868569788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)