FastQCFastQC Report
Thu 15 Sep 2022
EGAF00003947081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9185685
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7764748.453087603156433No Hit
CCCCCCACTGCTAAATTTGACTG4218214.592156164728053No Hit
AACATTCAACGCTGTCGGTGAGT3003683.2699575480761642No Hit
TCGTACGACTCTTAGCGG2757893.0023781568821484No Hit
AAGCTGCCAGTTGAAGAACTGT2521012.7444986410920906No Hit
TTCAAGTAATCCAGGATAGGCT2458162.676076961054075No Hit
AACATTCAACGCTGTCGGTGAG2078862.263151849862041No Hit
TAGCTTATCAGACTGATGTTGAC1981862.1575527573610462No Hit
TAGCAGCACGTAAATATTGGCG1862612.0277311926111117No Hit
CCCCCACTGCTAAATTTGACTG1599041.7407955966267077No Hit
GCCTGTCTGAGCGTCGCT1430211.5569987431530692No Hit
TATTGCACTTGTCCCGGCCTGT1204111.3108548790863175No Hit
TAGCTTATCAGACTGATGTTGA1191091.2966806503815447No Hit
TAGCTTATCAGACTGATGTTGACT1143891.2452963497006484No Hit
TATTGCACTTGTCCCGGCCTGTAA965771.0513859336565536No Hit
TGAGGTAGTAGGTTGTATAGTT942301.0258353078730655No Hit
TGTAAACATCCTTGACTGGAAGCT854190.9299143177672651No Hit
CATAAAGTAGAAAGCACTACT851430.9269096425579585No Hit
TATTGCACTTGTCCCGGCCTGTAT838940.9133123985854076No Hit
TATTGCACTTGTCCCGGCCTGTA636110.6925014302145132No Hit
ACTGGACTTGGAGTCAGAAGGC630960.686894880458017No Hit
TGTAAACATCCCCGACTGGAAGCT580450.6319071468268289No Hit
AGCAGCATTGTACAGGGCTATGA578670.6299693490469138No Hit
ACCGGGTGCTGTAGGCTT574380.6252990386672306No Hit
TGAGGTAGTAGATTGTATAGTT569280.6197469214326422No Hit
TTCAAGTAATTCAGGATAGGTT568630.6190392986478418No Hit
CCCATAAAGTAGAAAGCACTAC500630.54501106885333No Hit
CAACGGAATCCCAAAAGCAGCT455980.4964028267897277No Hit
CATTGCACTTGTCTCGGTCTGA441440.48057384941896003No Hit
CGCCTGTCTGAGCGTCGCT407780.4439298756706767No Hit
CAACGGAATCCCAAAAGCAGCTGT397450.4326841166445399No Hit
CCCATAAAGTAGAAAGCACT389760.4243123947751311No Hit
CAACACCAGTCGATGGGCTGT386390.4206436427985501No Hit
TCCTGTCTGAGCGTCGCT318180.34638679641202585No Hit
TCGTTTCCCGGCCAATGCACCA312910.34064960860295124No Hit
ATCCCGGACGAGCCCCCA268880.2927163298110048No Hit
ACCGGGTGCTGTAGGCTTT263880.2872730776202319No Hit
AACATTCAACGCTGTCGGTGAGTT257830.2806867424693967No Hit
TGAGGTAGTAGTTTGTACAGTT244340.26600084805869134No Hit
ACCGGGTGCTGTAGGCTTA233040.2536990981075445No Hit
TGAGGTAGTAGTTTGTGCTGTT229600.24995414060029275No Hit
TTCTCACTACTGCACTTGACTAG229420.24975818352142493No Hit
TAGCTTATCAGACTGATGTTGACA221430.24105986652056977No Hit
CCCCCACTGCTAAATTTGACTGGCTT205740.22397894114592432No Hit
CAAAGAATTCTCCTTTTGGGCT204610.22274876615080969No Hit
AACGGAATCCCAAAAGCAGCTG202670.22063678430078978No Hit
TTCACAGTGGCTAAGTTCCG200370.21813288829303423No Hit
TATTGCACTTGTCCCGGCCTGTT196680.2141157681762438No Hit
CACCCGGCTGTGTGCACATGTGC193440.21058854075662295No Hit
TGTAAACATCCTACACTCTCAGCT192070.20909708965635115No Hit
CACTAGATTGTGAGCTCCTGGA191410.2083785803671691No Hit
CTCCCACTGCTTCACTTGACTAG191310.20826971532335367No Hit
TGTCAGTTTGTCAAATACCCCAA182350.1985154073974886No Hit
ACTGGACTTGGAGTCAGAAGG177440.19317013374614958No Hit
CTTCTCACTACTGCACTTGACTAG177200.19290885764099247No Hit
TAGCAGCACGTAAATATTGGC171510.1867144366478929No Hit
ACTGGACTTGGAGTCAGAAGGCT159440.17357442585936705No Hit
CACTAGATTGTGAGCTCCTGGAA151290.16470192478840717No Hit
TGTAAACATCCCCGACTGGAAGC149540.16279678652163665No Hit
CTACGCCTGTCTGAGCGTCGCT147030.16006427392186864No Hit
ACTGGACTTGGAGTCAGAAGGCA146110.1590627155187664No Hit
ACCACAGGGTAGAACCACGGAC145680.15859459583035995No Hit
TTATAAAGCAATGAGACTGATT145250.1581264761419535No Hit
TGAGGTAGTAGGTTGTGTGGTT140750.15322754917025785No Hit
CAAAGAATTCTCCTTTTGGGCTT135860.1479040485276819No Hit
TTCAAGTAATCCAGGATAGGCTT134340.14624929986168697No Hit
ATCCCACCGCTGCCACCA133810.14567231512946502No Hit
TTCACAGTGGCTAAGTTCCGC131900.14359299279258977No Hit
TTCACAGTGGCTAAGTTCTG129570.1410564372716896No Hit
CACCCGTAGAACCGACCTTGCG127580.13889002289976196No Hit
CCCATAAAGTAGAAAGCACTA125090.13617928330875703No Hit
TACGCCTGTCTGAGCGTCGCT123400.1343394640682758No Hit
TTCAAGTAATCCAGGATAGGC122730.13361006827471222No Hit
TGTCAGTTTGTCAAATACCCCA119900.13052918753473475No Hit
TTCACAGTGGCTAAGTTCTGC117970.1284280921890964No Hit
AGCTCGGTCTGAGGCCCCTCAGT117660.12809061055326848No Hit
TATTGCACTTGTCCCGGCCTGTAG117620.1280470645357423No Hit
TACCTGGTTGATCCTGCC113710.12379044132255787No Hit
AGCAGCATTGTACAGGGCTATGT113210.12324611610348057No Hit
AGCAGCATTGTACAGGGCTATG112610.12259292584058783No Hit
CCCCCCACTGCTAAATTTGACTGG112300.12225544420475991No Hit
CCCCCCACTGCTAAATTTGACTGGCT111020.12086197164392204No Hit
ACCCCCCACTGCTAAATTTGACTG109280.11896771988153307No Hit
CAAAGTGCTGTTCGTGCAGGTAG105230.114558685607007No Hit
CGCGACCTCAGATCAGACGTGGCG102050.1110967772136754No Hit
CAACGGAATCCCAAAAGCAGCTGA101520.1105197924814535No Hit
TGAGGTAGTAGGTTGTATAGT101380.11036738142011183No Hit
TGATGTAGTAGGTTGTATAGTT100410.10931139049510188No Hit
TGTAAACATCCTACACTCAGCT98560.10729738718451591No Hit
AATGACACGATCACTCCCGTTG95700.10418384693139379No Hit
TCGTTTCCCGGGCGGCGCACCA93500.1017888159674537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)