FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10396748
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110574910.635527570736542No Hit
CAACGGAATCCCAAAAGCAGCTG8509228.18450153836565No Hit
TAGCAGCACGTAAATATTGGCG3344903.217256011206581No Hit
TTCAAGTAATCCAGGATAGGCT3283153.1578624392935177No Hit
AAGCTGCCAGTTGAAGAACTGT3055562.9389574509260012No Hit
CCCCCACTGCTAAATTTGACTG2984432.8705418271174796No Hit
AACATTCAACGCTGTCGGTGAGT2780082.673989982252143No Hit
GGAATACCGGGTGCTGTAGGCTTT2258212.172034947851001No Hit
TAGCTTATCAGACTGATGTTGACT1770601.7030325251703706No Hit
TAGCTTATCAGACTGATGTTGAC1746901.6802369356264093No Hit
AACATTCAACGCTGTCGGTGAG1706531.6414074862639743No Hit
TATTGCACTTGTCCCGGCCTGTAT1566751.506961599915666No Hit
TATTGCACTTGTCCCGGCCTGTAA1537741.4790586441067919No Hit
TGAATACCGGGTGCTGTAGGCTTT1522501.4644002143747257No Hit
GGAATACCGGGTGCTGTAGGCTT1404861.3512494483852067No Hit
TAGCTTATCAGACTGATGTTGA1116731.0741147135623563No Hit
TGTAAACATCCTTGACTGGAAGCT1012710.9740641977664555No Hit
TGAATACCGGGTGCTGTAGGCTT954120.9177100377925866No Hit
CATAAAGTAGAAAGCACTACT823810.7923727688696505No Hit
TATTGCACTTGTCCCGGCCTGT797820.7673745675089941No Hit
TTCAAGTAATTCAGGATAGGTT667590.642114245723759No Hit
TATTGCACTTGTCCCGGCCTGTA621080.5973791035427617No Hit
AGCAGCATTGTACAGGGCTATGA602470.5794792756350351No Hit
TGAGGTAGTAGGTTGTATAGTT575920.5539424443104709No Hit
TGTAAACATCCCCGACTGGAAGCT570420.548652328593518No Hit
CAACGGAATCCCAAAAGCAGCTGT551520.5304735673116248No Hit
CTTCTCACTACTGCACTTGACTAG489360.47068564131784285No Hit
TCGTACGACTCTTAGCGG451820.4345781969515852No Hit
TGAGGTAGTAGATTGTATAGTT429490.4131003271407559No Hit
TTCTCACTACTGCACTTGACTAG426930.4106380187343196No Hit
CCCATAAAGTAGAAAGCACTAC423020.40687722737917664No Hit
ACTGGACTTGGAGTCAGAAGGC403660.38825602005550197No Hit
GGAATACCGGGTGCTGTAGGCTTA396370.38124421213248605No Hit
CAACGGAATCCCAAAAGCAGCT383730.3690865643757067No Hit
CATTGCACTTGTCTCGGTCTGA367140.3531296516949339No Hit
TGTAAACATCCTACACTCTCAGCT343150.330055128776806No Hit
CCCATAAAGTAGAAAGCACT332920.3202155135432734No Hit
CTCCCACTGCTTCACTTGACTAG332860.3201578031899975No Hit
CAAAGAATTCTCCTTTTGGGCT330980.31834954545402083No Hit
TGAATACCGGGTGCTGTAGGCTTA277300.26671801605655926No Hit
CCCCCACTGCTAAATTTGACTGGCTT268440.25819612055615854No Hit
TAGCTTATCAGACTGATGTTGACA249030.23952682127142064No Hit
CAAAGAATTCTCCTTTTGGGCTT230260.22147309908829185No Hit
CCCCCCACTGCTAAATTTGACTGGCT219360.2109890515765122No Hit
ACCCCCCACTGCTAAATTTGACTG216200.20794963963731736No Hit
CACTAGATTGTGAGCTCCTGGA212460.20435236094978929No Hit
AACATTCAACGCTGTCGGTGAGTT209850.20184196058228976No Hit
CCCCCCACTGCTAAATTTGACTGG207080.19917766593938796No Hit
TAGCAGCACGTAAATATTGGC206850.1989564429184972No Hit
CACTAGATTGTGAGCTCCTGGAA204160.19636909541329656No Hit
GGAATACCGGGTGCTGTAGGCT201260.1935797616716304No Hit
TGAGGTAGTAGTTTGTACAGTT197780.19023256118163104No Hit
CTACGCCTGTCTGAGCGTCGCT189830.18258593937258075No Hit
TTCAAGTAATCCAGGATAGGCTT183940.17692070635933466No Hit
TGTAAACATCCCCGACTGGAAGC183500.17649749710197843No Hit
TATTGCACTTGTCCCGGCCTGTAG176710.1699666087895946No Hit
TTAATACCGGGTGCTGTAGGCTTT171960.1653978724885897No Hit
CTCCCACTGCTTCACTTGACTAGCCT170120.16362808832146358No Hit
TATTGCACTTGTCCCGGCCTGTT166670.16030974300810216No Hit
CAACACCAGTCGATGGGCTGT164950.15865537954752776No Hit
TGATGTAGTAGGTTGTATAGTT162840.15662589879066033No Hit
TGTCAGTTTGTCAAATACCCCAA154590.14869072521523077No Hit
TATTGCACTTGTCCCGGCCTGTTT150220.14448748781830625No Hit
TGTCAGTTTGTCAAATACCCCA150090.1443624487195419No Hit
AGCTCGGTCTGAGGCCCCTCAGT145070.13953401582879568No Hit
TTCAAGTAATCCAGGATAGGC141780.13636956479083653No Hit
ACTTGACTTGGAGTCAGAAGGC136720.13150265833123972No Hit
TATTGCACTTGTCCCGGCCTGTAAT135900.13071395016980308No Hit
TGAATACCGGGTGCTGTAGGCT135180.13002142593049287No Hit
AACGGAATCCCAAAAGCAGCTG135000.12984829487066532No Hit
TCGTTTCCCGGCCAATGCACCA131000.12600093798560857No Hit
AGCAGCATTGTACAGGGCTATGT130550.12556811033603968No Hit
CACCCGGCTGTGTGCACATGTGC128600.12369252385457452No Hit
GTAATACCGGGTGCTGTAGGCTTT126530.12170151666655767No Hit
TTCACAGTGGCTAAGTTCCGC126120.12130716258583933No Hit
ACTGGACTTGGAGTCAGAAGGCA124210.11947004967322475No Hit
TACGCCTGTCTGAGCGTCGCT123100.11840240813762148No Hit
TTATAAAGCAATGAGACTGATT122950.11825813225443188No Hit
TCGTTTCCCGGGCGGCGCACCA122560.11788301495813884No Hit
TGATGTAGTAGATTGTATAGTT122330.11766179193724807No Hit
CCCATAAAGTAGAAAGCACTA120630.11602666526109894No Hit
TGAGGTAGTAGTTTGTGCTGTT118010.11350664650138678No Hit
TTTAAACATCCTTGACTGGAAGCT115050.11065960240644478No Hit
ATGCACCTGGGCAAGGATTCTGA114610.11023639314908854No Hit
ACTGGACTTGGAGTCAGAAGGCT113420.10909180447578415No Hit
ATCACATTGCCAGGGATTTCCA112080.10780293991929014No Hit
ATCACATTGCCAGGGATTTCC109310.10513864527638836No Hit
TTCACAGTGGCTAAGTTCTGC109040.10487894868664702No Hit
TATTGCACTTGTCCCGGCCTGTAAA107620.10351313699245186No Hit
CAGTGCAATAGTATTGTCAAAGC107560.10345542663917602No Hit
CGCCTGTCTGAGCGTCGCT106450.10238778510357277No Hit
TTAATACCGGGTGCTGTAGGCTT105220.10120472286141782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)