FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791345
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6371589.381911830425343No Hit
CCCCCCACTGCTAAATTTGACTG3886315.722445259370566No Hit
AAGCTGCCAGTTGAAGAACTGT3035394.469497573750119No Hit
TAGCTTATCAGACTGATGTTGAC1719672.532149375418271No Hit
TGTAAACATCCTTGACTGGAAGCT1493072.1984894008476967No Hit
CCCCCACTGCTAAATTTGACTG1426612.1006295512891775No Hit
TAGCTTATCAGACTGATGTTGACT1366782.0125321272884826No Hit
TTCAAGTAATCCAGGATAGGCT1136861.6739835776271121No Hit
TGTAAACATCCCCGACTGGAAGCT1136691.6737332590230654No Hit
AACATTCAACGCTGTCGGTGAGT1108051.6315619365530685No Hit
TATTGCACTTGTCCCGGCCTGTAA991371.4597550264343808No Hit
AACATTCAACGCTGTCGGTGAG895551.3186636814946082No Hit
TATTGCACTTGTCCCGGCCTGTAT818891.205784715693283No Hit
ACTGGACTTGGAGTCAGAAGGC794671.1701216769285023No Hit
GGAATACCGGGTGCTGTAGGCTTT720881.0614686781484375No Hit
TAGCTTATCAGACTGATGTTGA712911.0497331530057743No Hit
TATTGCACTTGTCCCGGCCTGT618670.9109682986212598No Hit
GGAATACCGGGTGCTGTAGGCTT496680.7313426132820524No Hit
TAGCAGCACGTAAATATTGGCG487130.717280597584131No Hit
AGCAGCATTGTACAGGGCTATGA476050.7009657144497886No Hit
TATTGCACTTGTCCCGGCCTGTA472380.6955617775271319No Hit
CAACGGAATCCCAAAAGCAGCTGT337790.49738306624092876No Hit
CAAAGAATTCTCCTTTTGGGCT312090.45954078315856434No Hit
CACTAGATTGTGAGCTCCTGGA303950.44755493941185437No Hit
TGTAAACATCCCCGACTGGAAGC284070.41828238736214995No Hit
CACTAGATTGTGAGCTCCTGGAA277410.4084757879330236No Hit
TGAGAACTGAATTCCATGGGTT261420.3849311145288599No Hit
TGAATACCGGGTGCTGTAGGCTTT237630.3499012345860798No Hit
CATAAAGTAGAAAGCACTACT230600.33954982407755757No Hit
CCCATAAAGTAGAAAGCACTAC226430.333409655966528No Hit
CAAAGAATTCTCCTTTTGGGCTT224780.3309800930448976No Hit
TAGCTTATCAGACTGATGTTGACA203250.2992779780735627No Hit
TTAATGCTAATCGTGATAGGGGTT200920.29584714073574525No Hit
CAACACCAGTCGATGGGCTGT200800.2956704452505358No Hit
ACTGGACTTGGAGTCAGAAGGCT189580.2791494173834491No Hit
CACCCGGCTGTGTGCACATGTGC185700.27343626336167576No Hit
ACTTGACTTGGAGTCAGAAGGC178830.26332044683343286No Hit
TGAGGTAGTAGTTTGTGCTGTT165420.24357472636127303No Hit
ACCACAGGGTAGAACCACGGAC163890.24132185892485214No Hit
TGAATACCGGGTGCTGTAGGCTT163380.24057090311271184No Hit
CCCATAAAGTAGAAAGCACT162540.23933403471624545No Hit
TTTAAACATCCTTGACTGGAAGCT159580.2349755460810782No Hit
TGTAAACATCCTACACTCTCAGCT155000.2282316683955829No Hit
CATTGCACTTGTCTCGGTCTGA154450.22742181408837275No Hit
CCCCCACTGCTAAATTTGACTGGCTT145810.21469973915328996No Hit
TGAGGTAGTAGGTTGTATAGTT142960.2105032213795647No Hit
ACCGGGTGCTGTAGGCTT141620.20853012179472546No Hit
ACTGGACTTGGAGTCAGAAGG140860.20741105038839877No Hit
TATTGCACTTGTCCCGGCCTGTAG140810.20733742726956145No Hit
TCGTACGACTCTTAGCGG134590.19817871128620326No Hit
TGTAAACATCCTTGACTGGAAGC134270.19770752332564462No Hit
TGAGAACTGAATTCCATAGGCTGT125410.18466150666767775No Hit
TATTGCACTTGTCCCGGCCTGTT117550.1730879523864566No Hit
TTTAAACATCCCCGACTGGAAGCT116780.17195415635636238No Hit
CAAAGTGCTGTTCGTGCAGGTAG114400.16844969589970762No Hit
ACTGGACTTGGAGTCAGAAGGCA107330.15803938689611557No Hit
TTCAAGTAATTCAGGATAGGTT105400.15519753450899637No Hit
ATGCACCTGGGCAAGGATTCTGA102880.1514869293195972No Hit
TCAGTGCACTACAGAACTTTGT102550.15100101673527114No Hit
CGCCTGTCTGAGCGTCGCT101030.14876287392261767No Hit
AACGGAATCCCAAAAGCAGCTG98550.14511116722828837No Hit
TATTGCACTTGTCCCGGCCTGTAAA95300.14032566450386486No Hit
GCCTGTCTGAGCGTCGCT95270.14028149063256248No Hit
CAACGGAATCCCAAAAGCAGCT94340.13891210062218898No Hit
CCCCCCACTGCTAAATTTGACTGGCT93010.13695372566111721No Hit
GGAATACCGGGTGCTGTAGGCTTA88700.13060741281734325No Hit
TATTGCACTTGTCCCGGCCTGTAAT87720.12916439968813248No Hit
ACCGGGTGCTGTAGGCTTT87090.12823674839078267No Hit
GGGAATACCGGGTGCTGTAGGCTTT87060.12819257451948032No Hit
AGCTCGGTCTGAGGCCCCTCAGT85340.12565993923147772No Hit
GGAATACCGGGTGCTGTAGGCT84280.12409912911212728No Hit
GGGAATACCGGGTGCTGTAGGCTT83690.12323037630984732No Hit
CTACGCCTGTCTGAGCGTCGCT82000.12074191489314709No Hit
TGTAAACATCCCCGACTGGA81810.12046214704156541No Hit
TTCTCACTACTGCACTTGACTAG78760.11597113679249103No Hit
CTCCCACTGCTTCACTTGACTAG76390.11248140095960373No Hit
CTGGACTTGGAGTCAGAAGGC75890.11174516977123089No Hit
AACATTCAACGCTGTCGGTGAGTT74990.11041995363215976No Hit
TTATAAAGCAATGAGACTGATT74830.11018435965188045No Hit
TCAGTGCACTACAGAACTTTGTC73820.1086971726513673No Hit
TTCAAGTAATCCAGGATAGGCTT73050.10756337662127309No Hit
GTAAACATCCTTGACTGGAAGCT72310.1064737544624813No Hit
GAATACCGGGTGCTGTAGGCTTT69840.10283677239191942No Hit
AAAAGCTGGGTTGAGAGGGCGA68950.10152628087661573No Hit
TGAGGTAGTAGGTTGTGTGGTT68920.10148210700531338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)