FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22314046
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG243332810.904916123234667No Hit
CAACGGAATCCCAAAAGCAGCTG18065518.096026153213092No Hit
AACATTCAACGCTGTCGGTGAGT9698624.34641929123925No Hit
CCCCCACTGCTAAATTTGACTG7808533.4993788217520034No Hit
TTCAAGTAATCCAGGATAGGCT7298253.270697747956601No Hit
AACATTCAACGCTGTCGGTGAG7112143.1872928826981894No Hit
TAGCAGCACGTAAATATTGGCG5076322.2749437730835544No Hit
AAGCTGCCAGTTGAAGAACTGT4557932.042628217222462No Hit
TGAGGTAGTAGGTTGTATAGTT3136761.405733411143815No Hit
TATTGCACTTGTCCCGGCCTGTAA2861191.2822372061077583No Hit
TAGCTTATCAGACTGATGTTGACT2576621.1547076670900471No Hit
TAGCTTATCAGACTGATGTTGAC2573501.1533094446430736No Hit
TTAATGCTAATCGTGATAGGGGTT2542291.1393227386911364No Hit
TATTGCACTTGTCCCGGCCTGTAT2449051.0975373986411967No Hit
TAGCTTATCAGACTGATGTTGA2312971.0365533888385818No Hit
TATTGCACTTGTCCCGGCCTGT2097330.9399147066381418No Hit
TTCAAGTAATTCAGGATAGGTT1676690.7514056393000176No Hit
TGAGGTAGTAGATTGTATAGTT1623910.7277523762387152No Hit
TCGTACGACTCTTAGCGG1611240.7220743382889863No Hit
GCCTGTCTGAGCGTCGCT1345670.6030596154547678No Hit
TATTGCACTTGTCCCGGCCTGTA1309900.5870293536187924No Hit
TGAGAACTGAATTCCATGGGTT1123130.5033287105350593No Hit
ACTGGACTTGGAGTCAGAAGGC1115720.5000079322234973No Hit
AGCAGCATTGTACAGGGCTATGA1102210.4939534497688138No Hit
TGTAAACATCCTTGACTGGAAGCT1098280.4921922272634913No Hit
CATAAAGTAGAAAGCACTACT1068290.4787522621401784No Hit
TGTAAACATCCCCGACTGGAAGCT1044990.4683104086098953No Hit
GGAATACCGGGTGCTGTAGGCTTT1037900.465133037728792No Hit
CATTGCACTTGTCTCGGTCTGA947920.4248086608766515No Hit
CAACGGAATCCCAAAAGCAGCTGT853840.38264687632175715No Hit
GGAATACCGGGTGCTGTAGGCTT827760.370959170739363No Hit
TGAGAACTGAATTCCATAGGCTGT724340.3246116818079518No Hit
CAACACCAGTCGATGGGCTGT721100.3231596815745562No Hit
ATCCCGGACGAGCCCCCA712910.3194893476512507No Hit
AACATTCAACGCTGTCGGTGAGTT672560.3014065669668334No Hit
CCCATAAAGTAGAAAGCACT671990.3011511224813286No Hit
TTCTCACTACTGCACTTGACTAG647580.2902118244266414No Hit
TGAGGTAGTAGTTTGTACAGTT607740.2723575993345178No Hit
CGCCTGTCTGAGCGTCGCT589450.2641609683873557No Hit
CACTAGATTGTGAGCTCCTGGA573240.25689648573817586No Hit
CTCCCACTGCTTCACTTGACTAG526140.23578870456751772No Hit
AGCTCGGTCTGAGGCCCCTCAGT518480.23235588920090958No Hit
TGATGTAGTAGGTTGTATAGTT493760.22127766519796543No Hit
TGAGGTAGTAGTTTGTGCTGTT467440.20948240404272717No Hit
CCCATAAAGTAGAAAGCACTAC466150.20890429283869005No Hit
ACCGGGTGCTGTAGGCTT463110.2075419222493312No Hit
GAATACCGGGTGCTGTAGGCTTT460850.20652910727171578No Hit
CCCCCCACTGCTAAATTTGACTGG457650.20509503296712753No Hit
TGAGGTAGTAGGTTGTGTGGTT450660.2019624769080426No Hit
CACTAGATTGTGAGCTCCTGGAA442990.19852518005923264No Hit
GGGAATACCGGGTGCTGTAGGCTTT421440.18886758591427122No Hit
AACGGAATCCCAAAAGCAGCTG404890.1814507328702289No Hit
CTTCTCACTACTGCACTTGACTAG400580.17951921404123664No Hit
CCCGCCACTGCTAAATTTGACTG391860.1756113615612337No Hit
GGGAATACCGGGTGCTGTAGGCTT382070.17122399048563403No Hit
TTAATGCTAATCGTGATAGGGGT380450.17049799036893623No Hit
TAGCAGCACGTAAATATTGGC378940.16982128655645864No Hit
CAAAGAATTCTCCTTTTGGGCT374750.16794354551388843No Hit
ACCGGGTGCTGTAGGCTTA373270.16728028614801635No Hit
CCCCCACTGCTAAATTTGACTGGCTT371220.16636158229663953No Hit
ACCGGGTGCTGTAGGCTTT367070.16450176718287665No Hit
TGTAAACATCCTACACTCTCAGCT363470.1628884335902149No Hit
CACCCGGCTGTGTGCACATGTGC357630.16027124798434134No Hit
TATTGCACTTGTCCCGGCCTGTT357080.16002476646324024No Hit
GAATACCGGGTGCTGTAGGCTT353570.158451766210395No Hit
TATTGCACTTGTCCCGGCCTGTAG351880.15769439571828436No Hit
TTCAAGTAATCCAGGATAGGC342540.15350869134176742No Hit
GACCCGCGGGCGCTCTCCAGTCCT341030.15283198752928984No Hit
CCCCCCACTGCTAAATTTGACTGGCT340890.1527692467784641No Hit
TTCACAGTGGCTAAGTTCTG338160.15154580213736227No Hit
TAGCTTATCAGACTGATGTTGACA322460.14450987508047622No Hit
ACTGGACTTGGAGTCAGAAGGCT321390.14403035648487952No Hit
TTCAAGTAATCCAGGATAGGCTT301420.13508083652780853No Hit
ACCCCCCACTGCTAAATTTGACTG295750.13253983611936623No Hit
AACATTCAACGCTGTCGGTGA292710.13117746553000742No Hit
CTCCCACTGCTTCACTTGACTAGCCT292590.13112368774358538No Hit
AACCGGGCGGAAACACCA279760.1253739460786269No Hit
CAACGGAATCCCAAAAGCAGCT274830.12316457535312064No Hit
TGAGGTAGTAGGTTGTATAGT271620.12172601956633056No Hit
TGAGAACTGAATTCCATGGGTTG264700.11862483388265849No Hit
TTATAAAGCAATGAGACTGATT257910.11558190746761032No Hit
CAAGGGAATCCCAAAAGCAGCTG255640.11456461100779303No Hit
TGATGTAGTAGATTGTATAGTT254790.11418368502063678No Hit
TGTAAACATCCCCGACTGGAAGC254350.1139864998037559No Hit
CAAAGAATTCTCCTTTTGGGCTT252530.11317087004302133No Hit
CTACGCCTGTCTGAGCGTCGCT247390.11086738819127648No Hit
CCCCCCACTGCTAAATTTGACTGT246900.1106477955633864No Hit
TCGTTTCCCGGGCGGCGCACCA245970.11023101771861545No Hit
TTCACAGTGGCTAAGTTCCG244310.10948709167311028No Hit
GAGAAAGCTCACAAGAACTG237330.1063590170962272No Hit
ACTGGACTTGGAGTCAGAAGG234730.10519383172374924No Hit
AGCAGCATTGTACAGGGCTATGT229440.10282312763897682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)