FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261782
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6611008.00190564214839No Hit
CCCCCCACTGCTAAATTTGACTG6125637.414417373878904No Hit
AACATTCAACGCTGTCGGTGAGT3512454.251443574763894No Hit
TTCAAGTAATCCAGGATAGGCT2948703.5690847325673807No Hit
AAGCTGCCAGTTGAAGAACTGT2465282.9839567299161365No Hit
CCCCCACTGCTAAATTTGACTG2322172.8107374413897634No Hit
TAGCAGCACGTAAATATTGGCG2233002.7028067310418016No Hit
AACATTCAACGCTGTCGGTGAG2054852.48717528494458No Hit
TGAGGTAGTAGGTTGTATAGTT1160761.4049753430918415No Hit
CGCCTGTCTGAGCGTCGCT896791.085468001939533No Hit
TATTGCACTTGTCCCGGCCTGT886971.073581946364598No Hit
TATTGCACTTGTCCCGGCCTGTAA854711.034534680290523No Hit
CATAAAGTAGAAAGCACTACT817860.9899317120688975No Hit
TATTGCACTTGTCCCGGCCTGTAT765360.9263860992701091No Hit
TAGCTTATCAGACTGATGTTGA748350.9057973207233015No Hit
TAGCTTATCAGACTGATGTTGAC744390.9010041659293357No Hit
TAGCTTATCAGACTGATGTTGACT718340.8694734380548894No Hit
TGAGGTAGTAGATTGTATAGTT551170.6671321029772995No Hit
ACTGGACTTGGAGTCAGAAGGC495500.599749545558089No Hit
TATTGCACTTGTCCCGGCCTGTA447510.5416628034968727No Hit
TCGTACGACTCTTAGCGG415930.5034386044076206No Hit
TGTAAACATCCTTGACTGGAAGCT405810.4911894310452636No Hit
AGCAGCATTGTACAGGGCTATGA392400.47495806594751594No Hit
CCCATAAAGTAGAAAGCACTAC364590.4412970470535292No Hit
CAACGGAATCCCAAAAGCAGCTGT353890.4283458459688237No Hit
CCCATAAAGTAGAAAGCACT353860.4283095341900815No Hit
TTCAAGTAATTCAGGATAGGTT353130.42742594757402214No Hit
CAACACCAGTCGATGGGCTGT340710.41239287117476586No Hit
TGTAAACATCCCCGACTGGAAGCT335140.4056509842549707No Hit
CATTGCACTTGTCTCGGTCTGA313780.3797969977905493No Hit
ACCGGGTGCTGTAGGCTT301630.36509072739997256No Hit
GCCTGTCTGAGCGTCGCT291010.3522363577252462No Hit
TCCACCACGTTCCCGTGG290170.35121962792046557No Hit
ATCCCGGACGAGCCCCCA271600.3287426368790656No Hit
TGAGGTAGTAGTTTGTACAGTT263840.3193499901110923No Hit
CTCCCACTGCTTCACTTGACTAG248980.3013635557074733No Hit
AACATTCAACGCTGTCGGTGAGTT239110.28941698050130105No Hit
TTCTCACTACTGCACTTGACTAG235760.28536216520842594No Hit
CTACGCCTGTCTGAGCGTCGCT233230.2822998718678367No Hit
GGAATACCGGGTGCTGTAGGCTTT218390.26433764531671256No Hit
ATCCCACCGCTGCCACCA216860.26248574460086216No Hit
ACGCCTGTCTGAGCGTCGCT214470.25959290622773634No Hit
TACGCCTGTCTGAGCGTCGCT206940.25047864976345297No Hit
TGAGGTAGTAGTTTGTGCTGTT188620.22830425687823766No Hit
GGAATACCGGGTGCTGTAGGCTT184100.22283328221441817No Hit
AACGGAATCCCAAAAGCAGCTG180260.21818537453542103No Hit
ACCGGGTGCTGTAGGCTTA179370.2171081250994035No Hit
ACCGGGTGCTGTAGGCTTT170040.2058151619105902No Hit
TGAGGTAGTAGGTTGTGTGGTT169060.20462897713834616No Hit
CCCCCACTGCTAAATTTGACTGGCTT167790.20309177850492788No Hit
TTCACAGTGGCTAAGTTCCG167260.20245027041381627No Hit
CTTCTCACTACTGCACTTGACTAG164110.19863753364588896No Hit
TGTAAACATCCTACACTCTCAGCT163020.19731820568492367No Hit
TTCAAGTAATCCAGGATAGGC160980.19484900473045647No Hit
CCCCCCACTGCTAAATTTGACTGG148450.17968278514247893No Hit
TTCACAGTGGCTAAGTTCCGC142700.1727230275502307No Hit
TGTCAGTTTGTCAAATACCCCAA141480.17124634854804932No Hit
ACTGGACTTGGAGTCAGAAGGCT140470.1700238519970631No Hit
TAGCAGCACGTAAATATTGGC134590.16290674336359878No Hit
GCGTTCGCGCTTTCCCCTG134000.1621926117150029No Hit
TATTGCACTTGTCCCGGCCTGTT131330.1589608634069502No Hit
ACTGGACTTGGAGTCAGAAGG125400.15178323514224895No Hit
ACCACAGGGTAGAACCACGGAC125380.15175902728975418No Hit
CCCCCCACTGCTAAATTTGACTGGCT125250.15160167624853815No Hit
TTCACAGTGGCTAAGTTCTG124560.15076650533746835No Hit
CACCCGGCTGTGTGCACATGTGC122370.14811574548929032No Hit
CAAAGTGCTGTTCGTGCAGGT120370.14569496023981268No Hit
TGAGGTAGTAGGTTGTATAGT119890.14511397177993804No Hit
TTCACAGTGGCTAAGTTCTGC118650.1436130849252619No Hit
ACCCCCCACTGCTAAATTTGACTG117330.14201536666060663No Hit
CACTAGATTGTGAGCTCCTGGA110950.13429306171477293No Hit
CAAAGAATTCTCCTTTTGGGCT110350.13356682613992962No Hit
TTCAAGTAATCCAGGATAGGCTT110210.1333973711724662No Hit
AATGACACGATCACTCCCGTTG110070.13322791620500274No Hit
TAGCAGCACATCATGGTTTACA109360.1323685374414382No Hit
AACATTCAACGCTGTCGGTGA109280.13227170603145907No Hit
ACTGGACTTGGAGTCAGAAGGCA108030.13075871525053553No Hit
TTATAAAGCAATGAGACTGATT108010.13073450739804077No Hit
CAACGGAATCCCAAAAGCAGCT105980.12827741036982096No Hit
TGTAAACATCCCCGACTGGAAGC105720.12796270828738884No Hit
AGCTCGGTCTGAGGCCCCTCAGT105340.1275027590899881No Hit
GTCAGGATGGCCGAGCGGTCTAAGG105100.12721226486005077No Hit
TGTAAACATCCTACACTCAGCT104290.12623184683401234No Hit
TCGTTTCCCGGCCAATGCACCA101690.12308482600969137No Hit
AGAATTACTCTGAGACCT96820.11719021392721327No Hit
AACCGGGCGGAAACACCA93610.11330485360180165No Hit
TCCCCGGCACCTCCACCA93120.1127117612156796No Hit
AGAGGTAGTAGGTTGCATAGTT89960.10888692052150493No Hit
AGCAGCATTGTACAGGGCTATG89480.10830593206163029No Hit
TATTGCACTTGTCCCGGCCTGTAG88840.10753128078179744No Hit
CCCATAAAGTAGAAAGCACTA83440.10099516060820778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)