FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8752891
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG107725912.30746504212151No Hit
GCCTGTCTGAGCGTCGCT4938085.6416559968586375No Hit
TCGTACGACTCTTAGCGG4055824.633691885343939No Hit
TTCAAGTAATCCAGGATAGGCT3627894.1447905611985805No Hit
CCCCCCACTGCTAAATTTGACTG2721803.10960115920557No Hit
TAGCAGCACGTAAATATTGGCG2503022.8596494575335165No Hit
TGAGGTAGTAGATTGTATAGTT2306152.634729485378031No Hit
TAGCTTATCAGACTGATGTTGAC2296762.624001601299502No Hit
AAGCTGCCAGTTGAAGAACTGT2108972.4094553445255973No Hit
AACATTCAACGCTGTCGGTGAG1879442.147221986427113No Hit
TAGCTTATCAGACTGATGTTGA1825132.0851739156811164No Hit
TGAGGTAGTAGGTTGTATAGTT1462211.6705451947248058No Hit
CCCCCACTGCTAAATTTGACTG1379541.5760964006063825No Hit
CATAAAGTAGAAAGCACTACT1202211.373500481155312No Hit
TATTGCACTTGTCCCGGCCTGT1162911.3286010302196154No Hit
TAGCTTATCAGACTGATGTTGACT1064961.2166951467806466No Hit
TGAGGTAGTAGTTTGTACAGTT834600.953513530558075No Hit
TTCAAGTAATTCAGGATAGGTT699270.7989017571451534No Hit
AACATTCAACGCTGTCGGTGAGT656880.7504720440366504No Hit
TATTGCACTTGTCCCGGCCTGTAA617090.705012778063842No Hit
CATTGCACTTGTCTCGGTCTGA599850.6853164285948494No Hit
TGTAAACATCCTTGACTGGAAGCT579730.6623297376832409No Hit
TATTGCACTTGTCCCGGCCTGTAT558650.6382462662907604No Hit
CAACGGAATCCCAAAAGCAGCTGT460380.5259747893581675No Hit
TATTGCACTTGTCCCGGCCTGTA454330.5190627873693389No Hit
AGCAGCATTGTACAGGGCTATGA441210.5040734541307551No Hit
CCCATAAAGTAGAAAGCACT440170.5028852752764772No Hit
CAACACCAGTCGATGGGCTGT408820.4670685376980017No Hit
TTCACAGTGGCTAAGTTCCGC392160.4480348264362026No Hit
CAACGGAATCCCAAAAGCAGCT369590.4222490603390354No Hit
TTCTCACTACTGCACTTGACTAG355570.40623149539963427No Hit
AACCGGGCGGAAACACCA349600.39941089178421163No Hit
CACCCGGCTGTGTGCACATGTGC343060.3919390747582713No Hit
TGAGGTAGTAGATTGTATAGT315330.36025811357641724No Hit
TCCTGTCTGAGCGTCGCT300490.3433037153096046No Hit
AACGGAATCCCAAAAGCAGCTG288680.3298110304355441No Hit
TGAGAACTGAATTCCATAGGCTGT288440.32953683531532607No Hit
CTCCCACTGCTTCACTTGACTAG286970.3278573902039909No Hit
TGAGAACTGAATTCCATGGGTT265180.30296275824753216No Hit
CCCATAAAGTAGAAAGCACTAC263770.30135186191625146No Hit
TGTAAACATCCCCGACTGGAAGCT258370.2951824717113466No Hit
TGTCAGTTTGTCAAATACCCCA248950.28442031324279027No Hit
TGATGTAGTAGATTGTATAGTT247370.2826151953680218No Hit
TAGCTTATCAGACTGATGTTGACA240600.27488060801853925No Hit
ACTGGACTTGGAGTCAGAAGGC231900.264941034910637No Hit
TAGCAGCACGTAAATATTGGC228070.2605653377838248No Hit
TGGCTCAGTTCAGCAGGAAC223310.25512713456616787No Hit
TGTAAACATCCCCGACTGGA215000.24563312852861988No Hit
TCGTTTCCCGGCCAATGCACCA205730.23504234201019983No Hit
CTTCTCACTACTGCACTTGACTAG201120.22977551074267916No Hit
TTCACAGTGGCTAAGTTCTGC191980.2193332465810439No Hit
TGAGGTAGTAAGTTGTATTGTT190530.21767665106306017No Hit
TGTAAACATCCTACACTCTCAGCT190090.2171739600093272No Hit
TTCAAGTAATCCAGGATAGGC167780.1916852386257295No Hit
TGAGGTAGTAGTTTGTGCTGTT167730.19162811464235074No Hit
TGATGTAGTAGGTTGTATAGTT155630.17780411066469354No Hit
TTATAAAGCAATGAGACTGATT155460.1776098891212058No Hit
TTCACAGTGGCTAAGTTCTG144670.1652825335080718No Hit
CAAAGAATTCTCCTTTTGGGCT141860.1620721656421861No Hit
TATTGCACTTGTCCCGGCCTGTT137730.15735372461510147No Hit
CCCATAAAGTAGAAAGCACTA137570.15717092786828946No Hit
AGGTCCCTGTTCGGGCGCCA136690.15616554576082348No Hit
TAGCTTATCAGACTGATGTTG133590.15262385879134105No Hit
ATCCCACCGCTGCCACCA132570.15145852953041458No Hit
TTCACAGTGGCTAAGTTCCG127650.1458375295659457No Hit
TTCAAGTAATCCAGGATAGGCTT121760.13910832432392908No Hit
CTCGTACGACTCTTAGCGG119470.13649204588518238No Hit
CAAAGTGCTGTTCGTGCAGGTAG116620.1332359788325937No Hit
ATCACATTGCCAGGGATTTCC114540.13085962112403776No Hit
TGAGGTAGTAGGTTGTATAGT112330.12833474105869708No Hit
CCCCCACTGCTAAATTTGACTGGCTT112050.12801484675177607No Hit
ACCTGTCTGAGCGTCGCT107770.12312503377455516No Hit
CACTAGATTGTGAGCTCCTGGA106720.12192543012360145No Hit
TGTCAGTTTGTCAAATACCCCAA106110.12122851752638071No Hit
TCAGTGCACTACAGAACTTTGT106070.12118281833967773No Hit
AGAGGTAGTAGGTTGCATAGTT102520.11712701551978655No Hit
AATGCACCCGGGCAAGGATTCT102370.1169556435696503No Hit
TGAGGTAGTAGATTGTATAGTTA99460.11363102773700712No Hit
AGCTCGGTCTGAGGCCCCTCAGT98370.1123857248993504No Hit
CTCCCACTGCTTCACTTGACTAGCCT95870.10952952573041297No Hit
TGTCAGTTTGTCAAATACCCC94040.10743878793875075No Hit
TAGCAGCACATCATGGTTTACA93700.10705034485177527No Hit
TCGTTTCCCGGCCAACGCACCA92930.10617063550774253No Hit
AGCAGCATTGTACAGGGCTATGT92630.10582789160747004No Hit
TCCCACTGCTTCACTTGACTAG91830.10491390787341005No Hit
TGTCAGTTTGTCAAATACC90040.10286886926845085No Hit
CAAAGAATTCTCCTTTTGGGCTT87720.10021831643967691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)