FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10223591
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7058266.903895118652537No Hit
AAGCTGCCAGTTGAAGAACTGT4021573.933617845236571No Hit
AACATTCAACGCTGTCGGTGAGT3741793.659956662976835No Hit
GCCTGTCTGAGCGTCGCT3490243.413908087676825No Hit
TAGCAGCACGTAAATATTGGCG3051842.9850959413380287No Hit
TTCAAGTAATCCAGGATAGGCT2785862.724932951640964No Hit
CGCCTGTCTGAGCGTCGCT2763612.7031695614583953No Hit
AACATTCAACGCTGTCGGTGAG2642622.584825625360013No Hit
CCCCCCACTGCTAAATTTGACTG2392942.340606152965235No Hit
TAGCTTATCAGACTGATGTTGAC2372172.320290395028518No Hit
TAGCTTATCAGACTGATGTTGACT1552151.5182043178370497No Hit
TATTGCACTTGTCCCGGCCTGTAA1473721.441489590105864No Hit
TGAGGTAGTAGGTTGTATAGTT1292531.2642622342775645No Hit
TATTGCACTTGTCCCGGCCTGTAT1224711.1979254647413027No Hit
TATTGCACTTGTCCCGGCCTGT1112451.0881206026336538No Hit
AGCAGCATTGTACAGGGCTATGA1075141.0516265762196473No Hit
TAGCTTATCAGACTGATGTTGA1049711.0267527329682886No Hit
TGTAAACATCCTTGACTGGAAGCT1001200.9793036517208092No Hit
CCCCCACTGCTAAATTTGACTG937250.9167522448814707No Hit
TATTGCACTTGTCCCGGCCTGTA770790.7539327424189798No Hit
CATAAAGTAGAAAGCACTACT765610.7488660295584986No Hit
ACTGGACTTGGAGTCAGAAGGC692160.677022388708625No Hit
TCCTGTCTGAGCGTCGCT683830.6688745666762295No Hit
TACGCCTGTCTGAGCGTCGCT675220.6604528682729972No Hit
TTAATGCTAATCGTGATAGGGGTT665540.65098457088121No Hit
TGAGGTAGTAGATTGTATAGTT660050.6456146377530165No Hit
GCGTTCGCGCTTTCCCCTG600280.5871518138783134No Hit
TGTAAACATCCCCGACTGGAAGCT547750.5357706504495339No Hit
TGTCAGTTTGTCAAATACCCCAA505240.4941903485771291No Hit
TTCAAGTAATTCAGGATAGGTT495830.48498614625722014No Hit
CAACACCAGTCGATGGGCTGT481470.47094020095287453No Hit
CATTGCACTTGTCTCGGTCTGA470380.46009274040794473No Hit
AACCGGGCGGAAACACCA428860.4194807871324273No Hit
ACGCCTGTCTGAGCGTCGCT402710.3939026903560598No Hit
TGTAAACATCCTACACTCTCAGCT400990.3922203069352051No Hit
CAACGGAATCCCAAAAGCAGCTGT382450.37408577866622406No Hit
CCCATAAAGTAGAAAGCACTAC376280.36805071720885546No Hit
CTACGCCTGTCTGAGCGTCGCT370390.36228953212232373No Hit
CCCATAAAGTAGAAAGCACT345400.33784606602513734No Hit
AACATTCAACGCTGTCGGTGAGTT321030.31400904046337536No Hit
TGAGGTAGTAGTTTGTACAGTT318840.311866935991473No Hit
CACCCGGCTGTGTGCACATGTGC309340.302574701980938No Hit
ATCCCGGACGAGCCCCCA266740.2609063684179072No Hit
CAACGGAATCCCAAAAGCAGCT261030.2553212467126277No Hit
TAGCTTATCAGACTGATGTTGACA260790.2550864955376247No Hit
CCTCGACACAAGGGTTTGT256260.25065556710944326No Hit
TGAGGTAGTAGTTTGTGCTGTT256080.250479503728191No Hit
TGTCAGTTTGTCAAATACCCCA254930.24935465434796833No Hit
ACTGGACTTGGAGTCAGAAGGCA239500.23426211005506772No Hit
TGTAAACATCCTACACTCAGCT237740.23254060143837912No Hit
TTATAAAGCAATGAGACTGATT237180.2319928486967055No Hit
TAGCAGCACGTAAATATTGGC232220.22714132441331034No Hit
ACCACAGGGTAGAACCACGGAC231940.22686744804247352No Hit
AGCAGCATTGTACAGGGCTATGT223580.2186902821132027No Hit
TCGTACGACTCTTAGCGG219070.21427891628293816No Hit
TGAGGTAGTAGGTTGTGTGGTT206270.20175885361611196No Hit
AACGGAATCCCAAAAGCAGCTG201270.196868204136883No Hit
TCGTTTCCCGGCCAATGCACCA189040.18490567551068895No Hit
CCAAGTCCTTCTGATCGAGGCCC181730.1777555459720562No Hit
TTCAAGTAATCCAGGATAGGCTT178120.17422449704805287No Hit
AGCAGCATTGTACAGGGCTATG177640.1737549946980469No Hit
ACTGGACTTGGAGTCAGAAGGCT173370.16957838004278536No Hit
TACCTGGTTGATCCTGCC172030.168267685982352No Hit
CAAGTCCTTCTGATCGAGGCCC171280.16753408856046764No Hit
TATTGCACTTGTCCCGGCCTGTAG170570.16683961633441713No Hit
TTCACAGTGGCTAAGTTCCGC169890.166174488005242No Hit
TATTGCACTTGTCCCGGCCTGTT168120.16444319808959493No Hit
CACTAGATTGTGAGCTCCTGGA163870.1602861460322503No Hit
GGAATACCGGGTGCTGTAGGCTTT160090.1565888150259532No Hit
TTCACAGTGGCTAAGTTCCG158140.15468146172905392No Hit
TAGCAGCACGTAAATATTGGCGT155960.15254913855611008No Hit
CTGCGTTCGCGCTTTCCCCTG155490.15208941750506255No Hit
CAAAGTGCTGTTCGTGCAGGTAG152620.14928218470398513No Hit
ACTGGACTTGGAGTCAGAAGG151260.14795192804563484No Hit
TGTAAACATCCCCGACTGGAAGC150580.14728679971645972No Hit
TGAGAACTGAATTCCATAGGCTGT149540.1462695446247801No Hit
ATAGGCGATAGAAATTGAAACCT144150.14099742448617125No Hit
CAAAGAATTCTCCTTTTGGGCT140310.1372414056861234No Hit
CACTAGATTGTGAGCTCCTGGAA140200.13713381139758035No Hit
CTCGACACAAGGGTTTGT139030.13598939941944077No Hit
ATCCCACCGCTGCCACCA137810.1347960809465089No Hit
CAAAGAATTCTCCTTTTGGGCTT136680.13369079416420318No Hit
TTCAAGTAATCCAGGATAGGC129490.12665804021307192No Hit
CAACACCAGTCGATGGGCTGTC128460.12565056642035072No Hit
TCGTTCGCGCTTTCCCCTG125420.12267705153697953No Hit
GGAATACCGGGTGCTGTAGGCTT124430.12170870294009217No Hit
TGAGGTAGTAGGTTGTATAGT122430.1197524431484006No Hit
AGAGGTAGTAGGTTGCATAGTT118800.11620183162648036No Hit
AACATTCAACGCTGTCGGTGAGA115600.11307181595977382No Hit
ATCACATTGCCAGGGATTTCC113430.11094927408578845No Hit
TTCACAGTGGCTAAGTTCTGC113080.11060692862224242No Hit
CAAAGTGCTGTTCGTGCAGGT111610.1091690776753491No Hit
TTCTCACTACTGCACTTGACTAG110410.10799532180033415No Hit
ATCACATTGCCAGGGATTTCCA110380.10796597790345877No Hit
CACCCGTAGAACCGACCTTGCG109720.10732041217220055No Hit
TCCCCGGCACCTCCACCA109480.10708566099719755No Hit
TATTGCACTTGTCCCGGCCTGTAAT108990.10660637734823313No Hit
TCGTTTCCCGGGCGGCGCACCA107800.10544240277217663No Hit
AGCTCGGTCTGAGGCCCCTCAGT107610.10525655809196592No Hit
AACATTCAACGCTGTCGGTGA106660.10432733469091242No Hit
AATGACACGATCACTCCCGTTG105350.10304598452735443No Hit
ACCGGGTGCTGTAGGCTT103900.10162769617837802No Hit
TAGCAGCACATCATGGTTTACA102680.10043437770544615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)